公开(公告)号：US10590383B2

公开(公告)日：2020-03-17

申请号：US15678432

申请日：2017-08-16

Applicant: FUJIFILM Cellular Dynamics, Inc.

Inventor： Christopher W. McMahon ,  Lauren E. Little ,  Wen Bo Wang ,  Nathaniel A. Elliott

IPC: C12N5/00 ,  C12N5/0793 ,  A61K38/18 ,  C12N5/0797 ,  C07C215/28 ,  C07C229/36 ,  G01N33/50

Abstract: Methods are provided, in some aspects, for differentiating pluripotent cells into midbrain dopaminergic (DA) neurons using a mono-SMAD inhibition or inhibition of SMAD signaling with only one SMAD inhibitor. In some embodiments, mono-SMAD inhibition utilizes a single inhibitor of bone morphogenic protein (BMP) for differentiating pluripotent cells into midbrain DA neurons.