公开(公告)号：US10947502B2

公开(公告)日：2021-03-16

申请号：US15769386

申请日：2016-10-20

Applicant: FUJIFILM Cellular Dynamics, Inc.

Inventor： Maksym A. Vodyanyk ,  Xin Zhang ,  Andrew J. Brandl ,  Deepika Rajesh ,  Bradley Swanson ,  Christie Munn ,  Sarah A. Burton ,  Wen Bo Wang

IPC: C12N5/0783 ,  C12N5/0784 ,  C12N5/0789 ,  C12N5/0781 ,  C12N15/85 ,  C12N15/86

Abstract: Provided herein are methods for the efficient in vitro differentiation of somatic cell-derived pluripotent stem cells to hematopoietic precursor cells, and the further differentiation of the hematopoietic precursor cells into immune cells of various myeloid or lymphoid lineages, particularly T cells, NK cells, and dendritic cells. The pluripotent cells may be maintained and differentiated under defined conditions; thus, the use of mouse feeder cells or serum is not required in certain embodiments for the differentiation of the hematopoietic precursor cells.

公开(公告)号：US12129486B2

公开(公告)日：2024-10-29

申请号：US17063204

申请日：2020-10-05

Applicant: FUJIFILM Cellular Dynamics, Inc.

Inventor： Maksym A. Vodyanyk ,  Xin Zhang ,  Andrew J. Brandl ,  Deepika Rajesh ,  Bradley Swanson ,  Christie Munn ,  Sarah A. Burton ,  Wen Bo Wang

IPC: C12N5/0783 ,  C12N5/0781 ,  C12N5/0784 ,  C12N5/0789 ,  C12N15/85 ,  C12N15/86

CPC classification number: C12N5/0636 ,  C12N5/0635 ,  C12N5/0637 ,  C12N5/0638 ,  C12N5/0639 ,  C12N5/0646 ,  C12N5/0647 ,  C12N15/85 ,  C12N15/86 ,  C12N2500/38 ,  C12N2500/90 ,  C12N2501/115 ,  C12N2501/125 ,  C12N2501/145 ,  C12N2501/155 ,  C12N2501/165 ,  C12N2501/22 ,  C12N2501/2303 ,  C12N2501/2306 ,  C12N2501/2307 ,  C12N2501/25 ,  C12N2501/26 ,  C12N2501/60 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/605 ,  C12N2501/606 ,  C12N2501/608 ,  C12N2501/727 ,  C12N2501/998 ,  C12N2501/999 ,  C12N2506/025 ,  C12N2506/03 ,  C12N2506/09 ,  C12N2506/11 ,  C12N2506/45 ,  C12N2510/00 ,  C12N2533/50 ,  C12N2840/20

Abstract: Provided herein are methods for the efficient in vitro differentiation of somatic cell-derived pluripotent stem cells to hematopoietic precursor cells, and the further differentiation of the hematopoietic precursor cells into immune cells of various myeloid or lymphoid lineages, particularly T cells, NK cells, and dendritic cells. The pluripotent cells may be maintained and differentiated under defined conditions; thus, the use of mouse feeder cells or serum is not required in certain embodiments for the differentiation of the hematopoietic precursor cells.

公开(公告)号：US12163155B2

公开(公告)日：2024-12-10

申请号：US16606120

申请日：2018-04-18

Applicant: FUJIFILM Cellular Dynamics, Inc.

Inventor： Maksym A. Vodyanyk ,  Xin Zhang ,  Andrew J. Brandl ,  Deepika Rajesh ,  Bradley Swanson ,  Christie Munn ,  Sarah Burton ,  Wen Bo Wang ,  Ethan McLEOD

IPC: C12N5/0783 ,  A61K35/17 ,  A61K35/545 ,  C07K14/725

Abstract: Provided herein are methods for the production of antigen-specific effector T cells and NK cells from pluripotent stem cells which express a chimeric antigen receptor (CAR). Further provided herein are methods for the adoptive cell therapy by administering the effector T cells and/or NK cells provided herein.

公开(公告)号：US10828335B2

公开(公告)日：2020-11-10

申请号：US15614755

申请日：2017-06-06

Applicant: FUJIFILM Cellular Dynamics, Inc.

Inventor： Matt George ,  Carrie Chavez ,  Chris McMahon ,  Wen Bo Wang ,  Lucas Chase ,  Brad Swanson

IPC: A61K35/30 ,  B65D25/20 ,  G01N33/50 ,  C12N9/02 ,  C12Q1/68 ,  A61J1/00 ,  C12N5/0793 ,  C12Q1/6876

Abstract: Methods are provided for efficient production of midbrain dopaminergic (DA) neurons. In some aspects, methods involve differentiation and selection of DA neurons for a transgenic pluripotent cell population (e.g., cells comprising a selectable marker gene). Cell populations produced by the instant methods and methods of their use are likewise provided.

公开(公告)号：US10590383B2

公开(公告)日：2020-03-17

申请号：US15678432

申请日：2017-08-16

Applicant: FUJIFILM Cellular Dynamics, Inc.

Inventor： Christopher W. McMahon ,  Lauren E. Little ,  Wen Bo Wang ,  Nathaniel A. Elliott

IPC: C12N5/00 ,  C12N5/0793 ,  A61K38/18 ,  C12N5/0797 ,  C07C215/28 ,  C07C229/36 ,  G01N33/50

Abstract: Methods are provided, in some aspects, for differentiating pluripotent cells into midbrain dopaminergic (DA) neurons using a mono-SMAD inhibition or inhibition of SMAD signaling with only one SMAD inhibitor. In some embodiments, mono-SMAD inhibition utilizes a single inhibitor of bone morphogenic protein (BMP) for differentiating pluripotent cells into midbrain DA neurons.