公开(公告)号：US10947502B2

公开(公告)日：2021-03-16

申请号：US15769386

申请日：2016-10-20

Applicant: FUJIFILM Cellular Dynamics, Inc.

Inventor： Maksym A. Vodyanyk ,  Xin Zhang ,  Andrew J. Brandl ,  Deepika Rajesh ,  Bradley Swanson ,  Christie Munn ,  Sarah A. Burton ,  Wen Bo Wang

IPC: C12N5/0783 ,  C12N5/0784 ,  C12N5/0789 ,  C12N5/0781 ,  C12N15/85 ,  C12N15/86

Abstract: Provided herein are methods for the efficient in vitro differentiation of somatic cell-derived pluripotent stem cells to hematopoietic precursor cells, and the further differentiation of the hematopoietic precursor cells into immune cells of various myeloid or lymphoid lineages, particularly T cells, NK cells, and dendritic cells. The pluripotent cells may be maintained and differentiated under defined conditions; thus, the use of mouse feeder cells or serum is not required in certain embodiments for the differentiation of the hematopoietic precursor cells.

公开(公告)号：US12129486B2

公开(公告)日：2024-10-29

申请号：US17063204

申请日：2020-10-05

Applicant: FUJIFILM Cellular Dynamics, Inc.

Inventor： Maksym A. Vodyanyk ,  Xin Zhang ,  Andrew J. Brandl ,  Deepika Rajesh ,  Bradley Swanson ,  Christie Munn ,  Sarah A. Burton ,  Wen Bo Wang

IPC: C12N5/0783 ,  C12N5/0781 ,  C12N5/0784 ,  C12N5/0789 ,  C12N15/85 ,  C12N15/86

CPC classification number: C12N5/0636 ,  C12N5/0635 ,  C12N5/0637 ,  C12N5/0638 ,  C12N5/0639 ,  C12N5/0646 ,  C12N5/0647 ,  C12N15/85 ,  C12N15/86 ,  C12N2500/38 ,  C12N2500/90 ,  C12N2501/115 ,  C12N2501/125 ,  C12N2501/145 ,  C12N2501/155 ,  C12N2501/165 ,  C12N2501/22 ,  C12N2501/2303 ,  C12N2501/2306 ,  C12N2501/2307 ,  C12N2501/25 ,  C12N2501/26 ,  C12N2501/60 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/605 ,  C12N2501/606 ,  C12N2501/608 ,  C12N2501/727 ,  C12N2501/998 ,  C12N2501/999 ,  C12N2506/025 ,  C12N2506/03 ,  C12N2506/09 ,  C12N2506/11 ,  C12N2506/45 ,  C12N2510/00 ,  C12N2533/50 ,  C12N2840/20

Abstract: Provided herein are methods for the efficient in vitro differentiation of somatic cell-derived pluripotent stem cells to hematopoietic precursor cells, and the further differentiation of the hematopoietic precursor cells into immune cells of various myeloid or lymphoid lineages, particularly T cells, NK cells, and dendritic cells. The pluripotent cells may be maintained and differentiated under defined conditions; thus, the use of mouse feeder cells or serum is not required in certain embodiments for the differentiation of the hematopoietic precursor cells.