公开(公告)号：US10947502B2

公开(公告)日：2021-03-16

申请号：US15769386

申请日：2016-10-20

Applicant: FUJIFILM Cellular Dynamics, Inc.

Inventor： Maksym A. Vodyanyk ,  Xin Zhang ,  Andrew J. Brandl ,  Deepika Rajesh ,  Bradley Swanson ,  Christie Munn ,  Sarah A. Burton ,  Wen Bo Wang

IPC: C12N5/0783 ,  C12N5/0784 ,  C12N5/0789 ,  C12N5/0781 ,  C12N15/85 ,  C12N15/86

Abstract: Provided herein are methods for the efficient in vitro differentiation of somatic cell-derived pluripotent stem cells to hematopoietic precursor cells, and the further differentiation of the hematopoietic precursor cells into immune cells of various myeloid or lymphoid lineages, particularly T cells, NK cells, and dendritic cells. The pluripotent cells may be maintained and differentiated under defined conditions; thus, the use of mouse feeder cells or serum is not required in certain embodiments for the differentiation of the hematopoietic precursor cells.

公开(公告)号：US12129486B2

公开(公告)日：2024-10-29

申请号：US17063204

申请日：2020-10-05

Applicant: FUJIFILM Cellular Dynamics, Inc.

Inventor： Maksym A. Vodyanyk ,  Xin Zhang ,  Andrew J. Brandl ,  Deepika Rajesh ,  Bradley Swanson ,  Christie Munn ,  Sarah A. Burton ,  Wen Bo Wang

IPC: C12N5/0783 ,  C12N5/0781 ,  C12N5/0784 ,  C12N5/0789 ,  C12N15/85 ,  C12N15/86

CPC classification number: C12N5/0636 ,  C12N5/0635 ,  C12N5/0637 ,  C12N5/0638 ,  C12N5/0639 ,  C12N5/0646 ,  C12N5/0647 ,  C12N15/85 ,  C12N15/86 ,  C12N2500/38 ,  C12N2500/90 ,  C12N2501/115 ,  C12N2501/125 ,  C12N2501/145 ,  C12N2501/155 ,  C12N2501/165 ,  C12N2501/22 ,  C12N2501/2303 ,  C12N2501/2306 ,  C12N2501/2307 ,  C12N2501/25 ,  C12N2501/26 ,  C12N2501/60 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/605 ,  C12N2501/606 ,  C12N2501/608 ,  C12N2501/727 ,  C12N2501/998 ,  C12N2501/999 ,  C12N2506/025 ,  C12N2506/03 ,  C12N2506/09 ,  C12N2506/11 ,  C12N2506/45 ,  C12N2510/00 ,  C12N2533/50 ,  C12N2840/20

Abstract: Provided herein are methods for the efficient in vitro differentiation of somatic cell-derived pluripotent stem cells to hematopoietic precursor cells, and the further differentiation of the hematopoietic precursor cells into immune cells of various myeloid or lymphoid lineages, particularly T cells, NK cells, and dendritic cells. The pluripotent cells may be maintained and differentiated under defined conditions; thus, the use of mouse feeder cells or serum is not required in certain embodiments for the differentiation of the hematopoietic precursor cells.

公开(公告)号：US12163155B2

公开(公告)日：2024-12-10

申请号：US16606120

申请日：2018-04-18

Applicant: FUJIFILM Cellular Dynamics, Inc.

Inventor： Maksym A. Vodyanyk ,  Xin Zhang ,  Andrew J. Brandl ,  Deepika Rajesh ,  Bradley Swanson ,  Christie Munn ,  Sarah Burton ,  Wen Bo Wang ,  Ethan McLEOD

IPC: C12N5/0783 ,  A61K35/17 ,  A61K35/545 ,  C07K14/725

Abstract: Provided herein are methods for the production of antigen-specific effector T cells and NK cells from pluripotent stem cells which express a chimeric antigen receptor (CAR). Further provided herein are methods for the adoptive cell therapy by administering the effector T cells and/or NK cells provided herein.

公开(公告)号：US10961505B2

公开(公告)日：2021-03-30

申请号：US15725931

申请日：2017-10-05

Applicant: FUJIFILM Cellular Dynamics, Inc.

Inventor： Deepika Rajesh ,  Anne Strouse ,  Sarah Burton ,  Christie Munn ,  Bradley Swanson

IPC: C12N5/078 ,  C12N5/0783 ,  C12N5/071 ,  C12N5/079

Abstract: Provided herein are methods for the efficient in vitro maintenance, expansion, culture, and/or differentiation of pluripotent cells with disruption of the MeCP2 gene into various erythroid, myeloid, lymphoid, or endoderm lineages, particularly mature erythrocytes. The pluripotent cells may be maintained and differentiated under defined conditions; thus, the use of mouse feeder cells or serum is not required in certain embodiments for the differentiation of the precursor cells.