公开(公告)号：US11988667B2

公开(公告)日：2024-05-21

申请号：US17105638

申请日：2020-11-27

Applicant: FUJIFILM Corporation

Inventor： Hiyoku Nakata ,  Kousuke Watanabe ,  Akihiro Asakura ,  Keiko Makita ,  Yoshinori Kanazawa ,  Kazuhei Kaneko

IPC: C09K11/06 ,  C09B23/06 ,  G01N21/64 ,  G01N33/58

CPC classification number: G01N33/582 ,  C09B23/06 ,  C09K11/06 ,  G01N21/6428 ,  C09K2211/1029 ,  G01N2021/6439

Abstract: Provided are any one of the following fluorescent compounds and a fluorescent labeled biological substance having this fluorescent compound.






X represents CR5 or N, and R1 to R7, Q1, Q2, L1, and L2 each represent a specific group.
a ring β1 and a ring β2 are a 5- to 8-membered ring,
At least one of R1 to R7, L1, L2, Q1, or Q2 has a specific hydrophilic group.




However, in a case where the ring β1 and the ring β2 are a 6-membered ring, and L1 and L2 are an arylene group, R5 is not an aryl group substituted with a linear alkyl group having 18 or more carbon atoms. In addition to the regulation of the ring β1, the ring β2, L1, and L2, in a case where R5 has a substituent having a dipyrromethene boron complex structure, the dipyrromethene boron complex structure has a structure in which a dipyrromethene skeleton is coordinately bonded to a boron atom in the tridentate or tetradentate coordination.

公开(公告)号：US11893728B2

公开(公告)日：2024-02-06

申请号：US16928974

申请日：2020-07-14

Applicant: FUJIFILM Corporation

Inventor： Sohichiro Nakamura ,  Ryusuke Osaki ,  Sho Onozawa ,  Akihiro Asakura

IPC: G06T7/00 ,  G06T7/62 ,  C12Q1/02 ,  G01N15/06 ,  G01N21/45 ,  G02B21/16 ,  G03H1/04

CPC classification number: G06T7/0012 ,  C12Q1/02 ,  G01N15/06 ,  G01N21/453 ,  G06T7/62 ,  G01N2015/0693 ,  G01N2201/0633 ,  G01N2201/06113 ,  G02B21/16 ,  G03H1/0443 ,  G06T2207/10056 ,  G06T2207/10064 ,  G06T2207/30024

Abstract: Provided is a determination method capable of non-destructively and simply determining a state of a sphere that is an aggregate of a plurality of cells. A phase difference image of a sphere that is an aggregate of a plurality of cells is generated from a hologram obtained by imaging the sphere, and a state of the sphere is determined on the basis of the phase difference image and a shape index value corresponding to a shape of the sphere.