公开(公告)号：US20180023111A1

公开(公告)日：2018-01-25

申请号：US15724816

申请日：2017-10-04

申请人： CellTool GmbH

发明人： Raimund Schütze ,  Karin Schütze

IPC分类号： C12Q1/04 ,  G01N21/45 ,  C12Q1/02 ,  G01N21/65 ,  G03H1/04 ,  G01N15/14 ,  G01J3/44

CPC分类号： C12Q1/04 ,  C12Q1/025 ,  G01J3/44 ,  G01N15/1468 ,  G01N15/1484 ,  G01N21/453 ,  G01N21/65 ,  G01N2015/149 ,  G03H1/0443

摘要： In order to quantitatively characterize biological objects, for example individual cells, a stimulus is applied to a biological object (8) in a contactless fashion. A measurement and a further measurement are performed on the biological object (8) in order to ascertain a response of the biological object (8) to the stimulus, wherein the measurement and the further measurement comprise detecting Raman scattering on and/or in the biological object (8) and/or capturing data using digital holographic microinterferometry (DHMI). The biological object (8) is characterized according to a result of the measurement and is sorted if needed. The stimulus can be applied by means of a laser beam that creates optical tweezers or an optical trap, by means of ultrasonic waves or an electric or magnetic radio frequency field.

公开(公告)号：US20170299371A1

公开(公告)日：2017-10-19

申请号：US15513012

申请日：2015-09-18

申请人： NANOLIVE SA

发明人： Yann COTTE ,  Pierre-Alain COTTE ,  Sebastien EQUIS ,  Andreas KERN

IPC分类号： G01B9/02 ,  G03H1/26 ,  G03H1/08 ,  G02B21/36 ,  G02B21/14 ,  G01N21/45 ,  G02B26/08 ,  G03H1/00

CPC分类号： G01B9/02047 ,  G01B9/02068 ,  G01N21/453 ,  G02B21/14 ,  G02B21/367 ,  G02B26/0816 ,  G02B26/108 ,  G03H1/0443 ,  G03H1/0866 ,  G03H1/265 ,  G03H1/268 ,  G03H2001/0038 ,  G03H2001/005 ,  G03H2001/0456

摘要： Microscope (2) comprising a coherent light source (4) producing a coherent light beam (7), a light beam guide system (6) comprising a beam splitter (14) configured to split the coherent light beam (7) into a reference beam (7a) and a sample illumination beam (7b), a sample holder (18) configured to hold a sample (1) to be observed, a sample illumination device (28) configured to direct the sample illumination beam (7b) through the sample and into a microscope objective (37), a beam reuniter (16) configured to reunite the reference beam and sample illumination beam after passage of the sample illumination beam through the sample to be observed, and a light sensing system (8) configured to capture at least phase and intensity values of the coherent light beam downstream of the beam reuniter.

公开(公告)号：US20170191920A1

公开(公告)日：2017-07-06

申请号：US15376274

申请日：2016-12-12

申请人： New York University

发明人： Fook Chiong Cheong ,  Ke Xiao ,  David Pine ,  David G. Grier

IPC分类号： G01N15/08 ,  G02B21/36 ,  G01N21/45 ,  G01N15/14

CPC分类号： G01N15/088 ,  G01N15/08 ,  G01N15/1463 ,  G01N21/4133 ,  G01N21/453 ,  G01N21/47 ,  G01N2015/0846 ,  G02B21/367

摘要： A method for analyzing porosity of a particle and a medium disposed in the porosity of the particle. A video-holographic microscope is provided to analyze interference patterns produced by providing a laser source to output a collimated beam, scattering the collimated beam off a particle and interacting with an unscattered beam to generate the interference pattern for analyzation to determine the refractive index of the particle and a medium disposed in the porosity of the particle to measure porosity and the medium.

公开(公告)号：US20170131682A1

公开(公告)日：2017-05-11

申请号：US15359108

申请日：2016-11-22

申请人： PURDUE RESEARCH FOUNDATION

发明人： David D. Nolte

IPC分类号： G03H1/04 ,  G01N21/27 ,  G01N21/47 ,  G02B21/00

CPC分类号： G03H1/0443 ,  G01N21/453 ,  G01N21/4795 ,  G01N2021/479 ,  G03H2001/045

摘要： Motility contrast imaging (MCI) is a depth-resolved holographic technique to extract cellular and subcellular motion inside tissue. The holographic basis of the measurement technique makes it highly susceptible to mechanical motion. The motility contrast application, in particular, preferably includes increased mechanical stability because the signal is based on time-varying changes caused by cellular motion, which should not be confused with mechanical motion of the system. Apparatus for motility contrast imaging that provides increased mechanical stability are disclosed. It is based on common-path configurations, in which the signal and reference beams share optical elements in their paths to the detector. The two beams share mechanical motions in common, and hence those motions do not contribute to the signal.

公开(公告)号：US20170023472A1

公开(公告)日：2017-01-26

申请号：US15062092

申请日：2016-03-05

申请人： ECOLE POLYTECHNIQUE FEDERALE DE LAUSANNE (EPFL)

发明人： Nicolas PAVILLON ,  Christian DEPEURSINGE

IPC分类号： G01N21/45 ,  G01N21/47 ,  G01J9/00 ,  G03H1/04 ,  G02B21/36 ,  G02B21/08 ,  G02B21/02 ,  G02B21/00 ,  G01B9/02 ,  G03H1/00

CPC分类号： G01N21/453 ,  A61B5/0066 ,  A61B5/0073 ,  G01B9/02041 ,  G01B9/02083 ,  G01J9/00 ,  G01N21/45 ,  G01N21/4795 ,  G01N2021/458 ,  G01N2201/12 ,  G02B21/0092 ,  G02B21/02 ,  G02B21/088 ,  G02B21/365 ,  G03H1/0005 ,  G03H1/0443 ,  G03H1/0866 ,  G03H2001/005 ,  G03H2001/0456

摘要： The disclosed invention describes a new apparatus performing a new data acquisition for quantitative refractive index tomography. It is based on a linear scanning of the specimen, opposed to the classical approaches based on rotations of either the sample or the illumination beam, which are based on the illumination with plane waves, which orientation is successively modified in order to acquire angular information. On the contrary, the inventive apparatus and method rely on a specially shaped illumination, which provides straightforwardly an angular distribution in the illumination of the specimen. The specimen can thus be linearly scanned in the object plane in order to acquire the data set enabling tomographic reconstruction, where the different positions directly possess the information on various angles for the incoming wave vectors.

摘要翻译： 所公开的发明描述了一种执行定量折射层析成像的新数据采集的新装置。 它基于样本的线性扫描，与基于采用平面波照明的样本或照明光束的旋转的经典方法相反，该方向被连续修改以获取角度信息。 相反，本发明的装置和方法依赖于特殊形状的照明，其在样本的照明中提供直接的角度分布。 因此，样本可以在对象平面中线性扫描，以便获取能够进行断层重建的数据集，其中不同的位置直接拥有关于入射波矢量的各种角度的信息。

公开(公告)号：US20150292858A1

公开(公告)日：2015-10-15

申请号：US14442910

申请日：2013-11-15

申请人： CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE-CNRS

发明人： Michael Atlan ,  Benjamin Samson

IPC分类号： G01B9/021 ,  G01N21/45 ,  G03H1/00

CPC分类号： G01B9/021 ,  G01N21/453 ,  G03H1/0005 ,  G03H1/0443 ,  G03H1/0866 ,  G03H2001/0033 ,  G03H2001/0456 ,  G03H2001/0463 ,  G03H2210/62 ,  G03H2227/03

摘要： The invention relates to a digital holography method for detecting the vibration amplitude of an object (15) having a vibration frequency ω, comprising: generating object illumination waves (Wt) and reference waves (WLO); acquiring interferograms between the reference wave (WLO) and a signal wave (W≯) by means of a bandwidth a s detector (19), the reference wave comprising two components ELO, ELO1 of frequencies ω1, ω2 that are respectively staggered in relation to the laser frequency ωL by a quantity δ1=γ1ω, and δ2=qω+γ2ωδ, where q is an integer and −0.5≦γ1, γ2≦0.5; and calculating the vibration amplitude of the object from the optical beats spectrum deduced from the complex amplitude of an interferogram.

摘要翻译： 本发明涉及一种用于检测具有振动频率ω的物体（15）的振幅的数字全息方法，包括：产生物体照射波（Wt）和参考波（WLO）; 通过作为检测器（19）的带宽，在参考波（WLO）和信号波（W≯）之间获取干涉图，该参考波包括频率ω1，ω2的两个分量EL0，ELO1，它们分别相对于 激光频率ωL乘以δ1=γ1ω，δ2=qω+γ2ωδ，其中q为整数，-0.5＆nlE;γ1，γ2＆nlE; 0.5; 并从干涉图的复振幅推导出的光拍频谱中计算物体的振幅。

公开(公告)号：US20150260650A1

公开(公告)日：2015-09-17

申请号：US14339836

申请日：2014-07-24

申请人： SOLYMAN ASHRAFI ,  ROGER LINQUIST ,  NIMA ASHRAFI

发明人： SOLYMAN ASHRAFI ,  ROGER LINQUIST ,  NIMA ASHRAFI

IPC分类号： G01N21/59 ,  G01N33/49

CPC分类号： G01N21/59 ,  G01N21/17 ,  G01N21/21 ,  G01N21/453 ,  G01N24/00 ,  G01N33/483 ,  G01N33/50

摘要： Signal generation circuitry generates a first signal having at least one orbital angular momentum applied thereto and applies the first signal to the sample. A detector for receives the first signal after it passes through the sample and determines the concentration of the material within the sample based on a detected value of orbital angular momentum with the first signal received from the sample.

摘要翻译： 信号产生电路产生具有施加到其上的至少一个轨道角动量的第一信号，并将第一信号施加到样本。 一种检测器，用于在其通过样品之后接收第一信号，并且基于从样品接收到的第一信号的轨道角动量的检测值确定样品内的材料的浓度。

公开(公告)号：US20130280752A1

公开(公告)日：2013-10-24

申请号：US13976197

申请日：2012-01-05

申请人： Aydogan Ozcan ,  Serhan O. Isikman ,  Waheb Bishara

发明人： Aydogan Ozcan ,  Serhan O. Isikman ,  Waheb Bishara

IPC分类号： G01B9/02

CPC分类号： G01B9/02047 ,  G01B9/02041 ,  G01B9/02091 ,  G01N21/453 ,  G01N21/4795 ,  G02B21/0008 ,  G02B21/06 ,  G02B21/367 ,  G03H1/0005 ,  G03H1/02 ,  G03H1/0443 ,  G03H1/0866 ,  G03H1/265 ,  G03H2001/005 ,  G03H2001/0212 ,  G03H2001/0447 ,  G03H2001/046 ,  G03H2210/30 ,  G03H2210/62 ,  G03H2222/24 ,  G03H2222/34 ,  G03H2223/16 ,  G03H2227/02 ,  G03H2227/03 ,  G03H2240/56

摘要： A system for three dimensional imaging of an object contained within a sample includes an image sensor, a sample holder configured to hold the sample, the sample holder disposed adjacent to the image sensor, and an illumination source comprising partially coherent light. The illumination source is configured to illuminate the sample through at least one of an aperture, fiber-optic cable, or optical waveguide interposed between the illumination source and the sample holder, wherein the illumination source is configured to illuminate the sample through a plurality of different angles.

摘要翻译： 包含在样品中的物体的三维成像系统包括图像传感器，被配置为保持样本的样本保持器，与图像传感器相邻设置的样本保持器，以及包括部分相干光的照明源。 照明源被配置为通过插入在照明源和样本保持器之间的孔径，光纤电缆或光波导中的至少一个来照亮样本，其中照明源被配置为通过多个不同的 角度。

公开(公告)号：US20120182561A1

公开(公告)日：2012-07-19

申请号：US13327581

申请日：2011-12-15

申请人： Takahiro Masumura

发明人： Takahiro Masumura

IPC分类号： G01B11/24 ,  G02F1/11

CPC分类号： A61B5/0095 ,  G01N21/1702 ,  G01N21/1717 ,  G01N21/453 ,  G01N21/4795 ,  G01N21/6456 ,  G01N29/2418 ,  G01N2021/1729 ,  G01N2021/1787 ,  G01N2021/4186 ,  G03H1/0443 ,  G03H1/2294 ,  G03H2001/0463 ,  G03H2001/0467

摘要： A method for irradiating a medium includes irradiating the medium with an electromagnetic wave which is scattered in the medium and modulated in frequency at a position in the medium; obtaining information corresponding to an interference pattern generated by interference between the modulated electromagnetic wave and a reference wave; and generating a phase conjugate wave, based on the obtained information, which irradiates the medium.

摘要翻译： 用于照射介质的方法包括用介质中散射的电磁波照射介质并频率调制在介质中的位置; 获得与由调制电磁波和参考波之间的干扰产生的干扰图形相对应的信息; 并且基于获得的照射介质的信息产生相位共轭波。

公开(公告)号：US20100231895A1

公开(公告)日：2010-09-16

申请号：US12405063

申请日：2009-03-16

申请人： Christopher J. Mann ,  Philip R. Bingham ,  Shaun S. Gleason

发明人： Christopher J. Mann ,  Philip R. Bingham ,  Shaun S. Gleason

IPC分类号： G01N21/00

CPC分类号： G01N21/45 ,  G01B9/02007 ,  G01B9/02012 ,  G01B9/02024 ,  G01B9/02027 ,  G01B9/02069 ,  G01B9/021 ,  G01B11/0675 ,  G01N21/453 ,  G01N21/6458 ,  G01N2021/1787

摘要： An optical system performs imaging in a transmissive and reflective mode. The system includes an optical interferometer that generates interference phenomena between optical waves to measure multiple distances, thicknesses, and indices of refraction of a sample. Measurements are made through a galvanometer that scans a pre-programmed angular arc. An excitation-emission device allows an electromagnetic excitation and emission to pass through an objective in optical communication with the sample. An electromagnetic detector receives the output of the optical interferometer and the excitation-emission device to render a magnified three dimensional image of the sample.

摘要翻译： 光学系统以透射和反射模式执行成像。 该系统包括光学干涉仪，其产生光波之间的干涉现象，以测量样品的多个距离，厚度和折射率。 通过扫描预编程角弧的电流计进行测量。 激发发射装置允许电磁激发和发射通过与样品光学通信的物镜。 电磁检测器接收光干涉仪和激发发射装置的输出，以呈现样品的放大三维图像。