公开(公告)号：US11523592B2

公开(公告)日：2022-12-13

申请号：US17225127

申请日：2021-04-08

Applicant: FUJIFILM CORPORATION

Inventor： Yutaka Maeno ,  Kazutaka Chibana ,  Hidehiro Mochizuki ,  Tadanori Yamada

IPC: A01K61/54 ,  A01K61/57 ,  C07K14/325 ,  C07K14/78

Abstract: There are provided a pearl culture material containing at least one selected from the group consisting of a pearl nucleus and a mantle, and the at least one selected from the group consisting of a pearl nucleus and a mantle contains a protein having 10 EU/g or less of an endotoxin amount, a nucleus insertion method, and a pearl culture material composition.

公开(公告)号：US20200024565A1

公开(公告)日：2020-01-23

申请号：US16570327

申请日：2019-09-13

Applicant: FUJIFILM Corporation

Inventor： Toshiki TAKEI ,  Tadanori Yamada ,  Kenichiro Hata

IPC: C12M1/26 ,  C12N5/078

Abstract: An object of the present invention is to provide a method for efficiently separating megakaryocytes and platelets produced from the megakaryocytes, and a platelet separation kit for efficiently separating megakaryocytes and platelets produced from the megakaryocytes. According to the present invention, a method for separating megakaryocytes and platelets, including an incorporation step of incorporating magnetic fine particles into at least megakaryocytes; a culture step of culturing the megakaryocytes in a culture solution to produce platelets before and/or after the incorporation step; a magnetic field application step of applying a magnetic field to the culture solution after the incorporation step and the culture step; and a recovery step of recovering the culture solution after the magnetic field application step, is provided.

公开(公告)号：US20200048597A1

公开(公告)日：2020-02-13

申请号：US16659242

申请日：2019-10-21

Applicant: FUJIFILM Corporation

Inventor： Toshiki TAKEI ,  Tadanori Yamada ,  Ryuta Takegami ,  Kuniyuki Kaminaga ,  Akihito Fukunaga ,  Kazuhide Kanemura

IPC: C12M1/00 ,  B01D39/18 ,  C12N5/078

Abstract: An object of the present invention is to provide a separation substrate having a high megakaryocyte blocking rate and a high platelet permeation rate, and a cell separation filter and a method for producing a platelet which use the same. The separation substrate of the present invention is a separation substrate including non-woven fabric for separating a platelet from a cell suspension containing a megakaryocyte and the platelet, in which an average pore diameter of the separation substrate is 2.0 μm to 15.0 μm, and a thickness of the separation substrate is 10 μm to 500 μm.

公开(公告)号：US11512275B2

公开(公告)日：2022-11-29

申请号：US16659242

申请日：2019-10-21

Applicant: FUJIFILM Corporation

Inventor： Toshiki Takei ,  Tadanori Yamada ,  Ryuta Takegami ,  Kuniyuki Kaminaga ,  Akihito Fukunaga ,  Kazuhide Kanemura

IPC: B01D39/18 ,  C12M1/00 ,  C12N5/078 ,  C12N5/00 ,  C12M3/06

Abstract: An object of the present invention is to provide a separation substrate having a high megakaryocyte blocking rate and a high platelet permeation rate, and a cell separation filter and a method for producing a platelet which use the same. The separation substrate of the present invention is a separation substrate including non-woven fabric for separating a platelet from a cell suspension containing a megakaryocyte and the platelet, in which an average pore diameter of the separation substrate is 2.0 μm to 15.0 μm, and a thickness of the separation substrate is 10 μm to 500 μm.