公开(公告)号：US20250019420A1

公开(公告)日：2025-01-16

申请号：US18899965

申请日：2024-09-27

Applicant: FUJIFILM Corporation

Inventor： Takashi KUROSAWA ,  Atsushi INADA ,  Shinichi NAKAI

IPC: C07K16/00

Abstract: An object of the present invention is to provide a method that makes it possible to suppress membrane clogging in a production method for a product by cell culture, and a product that is produced by the method. According to the present invention, there is provided a production method for a product, which includes culturing cells, where the production method for a product is perfusion culture in which a cell density at a time of production of the product is 80×106 cells/mL or more and 300×106 cells/mL or less, where a period of the perfusion culture is 13 days or more and 500 days or less, and in a period of at least one day or more in the period of the perfusion culture, X [mol/L/day], which is an alkali addition rate per day, is controlled in a range of 0≤X

公开(公告)号：US20230348836A1

公开(公告)日：2023-11-02

申请号：US18347733

申请日：2023-07-06

Applicant: FUJIFILM Corporation

Inventor： Takashi KUROSAWA ,  Makoto KOIKE ,  Shinichi NAKAI

IPC: C12M1/42 ,  C12N5/071 ,  C12N5/075

CPC classification number: C12M35/04 ,  C12N5/0687 ,  C12N5/0609 ,  C12N2527/00

Abstract: An object of the present invention is to provide a method for selecting cells, an apparatus for selecting cells, and an apparatus system for selecting cells, which enables evaluation of a damage resistance of the cells in a suspension culture system. According to the present invention, provided is a method for selecting cells including introducing cells into a micro flow passage and allowing the cells to pass through the micro flow passage, evaluating a damage resistance of the cells to damage received by the cells due to passing through the micro flow passage, and selecting the cells based on the evaluation of the damage resistance.