METHOD FOR SELECTING CELLS, APPARATUS, AND APPARATUS SYSTEM

    公开(公告)号:US20230348836A1

    公开(公告)日:2023-11-02

    申请号:US18347733

    申请日:2023-07-06

    CPC classification number: C12M35/04 C12N5/0687 C12N5/0609 C12N2527/00

    Abstract: An object of the present invention is to provide a method for selecting cells, an apparatus for selecting cells, and an apparatus system for selecting cells, which enables evaluation of a damage resistance of the cells in a suspension culture system. According to the present invention, provided is a method for selecting cells including introducing cells into a micro flow passage and allowing the cells to pass through the micro flow passage, evaluating a damage resistance of the cells to damage received by the cells due to passing through the micro flow passage, and selecting the cells based on the evaluation of the damage resistance.

    METHOD FOR PRODUCING PRODUCT
    4.
    发明申请

    公开(公告)号:US20200216792A1

    公开(公告)日:2020-07-09

    申请号:US16807740

    申请日:2020-03-03

    Abstract: A method for producing a product related to the specified technology includes adjusting the concentration of cells in a culture vessel to a value of from 3×107 cells/ml to 3×108 cells/ml; in a case in which the average diameter of single cells in the culture vessel is designated as A, adjusting the number proportion of cells having a single cell diameter of 1.4×A or greater in the culture vessel to 5% or less, and adjusting the number proportion of cells having a single cell diameter in the range of A±A/7 to 50% or more.

    PRODUCTION METHOD OF PRODUCT AND PRODUCT

    公开(公告)号:US20250019420A1

    公开(公告)日:2025-01-16

    申请号:US18899965

    申请日:2024-09-27

    Abstract: An object of the present invention is to provide a method that makes it possible to suppress membrane clogging in a production method for a product by cell culture, and a product that is produced by the method. According to the present invention, there is provided a production method for a product, which includes culturing cells, where the production method for a product is perfusion culture in which a cell density at a time of production of the product is 80×106 cells/mL or more and 300×106 cells/mL or less, where a period of the perfusion culture is 13 days or more and 500 days or less, and in a period of at least one day or more in the period of the perfusion culture, X [mol/L/day], which is an alkali addition rate per day, is controlled in a range of 0≤X

    CELL CULTURE DEVICE, METHOD FOR CULTURING CELLS, AND METHOD FOR PRODUCING PRODUCT

    公开(公告)号:US20210355430A1

    公开(公告)日:2021-11-18

    申请号:US17387091

    申请日:2021-07-28

    Abstract: In the cell culture device, a volume of a space defined by a diaphragm of the diaphragm pump and a plane at an outer edge of the diaphragm is 1 cm3 or more and 20 cm3 or less, in a case where an angle formed by a straight line connecting a foot of a perpendicular line from the apex to the plane to each point in the diaphragm and by the perpendicular line is denoted by an angle A, a thickness H of the diaphragm is within a range of 0.5 mm or more and 1.5 mm or less where the angle A is from 0° to 75°, and in a case where a thickness of the diaphragm at the apex is denoted by HT, the thickness H satisfies a relationship of 1≤HT/H≤1.75 at any point where the angle A is from 0° to 75°.

    PRODUCTION METHOD FOR PRODUCT AND PRODUCT

    公开(公告)号:US20250019419A1

    公开(公告)日:2025-01-16

    申请号:US18898929

    申请日:2024-09-27

    Abstract: An object of the present invention is to provide a method in which the amount of mannose 5 in a product can be reduced in the production method for the product by cell culture, and a product that is produced by the method. According to the present invention, there is provided a production method for a product, including culturing cells in a culture container, in which the culturing is a perfusion culture method, Expressions (1) to (5) are satisfied in a production period of a product after reaching a target viable cell density, a variation of VCD during the production period is ±20% or less with respect to an average viable cell density during the production period, and a variation of VCV during the production period is ±20% or less with respect to an average value of the VCV during the production period. 0≤Gln-C≤2.5  Expression (1) 0.5≤Gluc-C≤8.0  Expression (2) 1.0≤NH4-C≤8.0  Expression (3) 50≤VCD≤300  Expression (4) 0.6×105≤VCV≤4.0×105  Expression (5) The meanings of the symbols in the expressions are as defined in the present specification.

    CELL CULTURE DEVICE, CELL CULTURE METHOD, AND PRODUCTION METHOD OF PRODUCT

    公开(公告)号:US20220403308A1

    公开(公告)日:2022-12-22

    申请号:US17892655

    申请日:2022-08-22

    Abstract: A cell culture device (1) includes: a culture vessel (10 ) that accommodates a cell suspension containing cells and a culture medium; and a stirring device (30) that is provided at a bottom part of the culture vessel (10) to stir the cell suspension accommodated in the culture vessel (10). The stirring device (30) has a shaft part (32), and a rotating part (33) rotatable with the shaft part (32) as a rotation axis. The rotating part (33) has a hole part (36) into which the shaft part (32) is inserted, and a gap (37) of 100 μm or more is secured between an interior wall demarcating the hole part (36) and the shaft part (32). A closing part (38) that closes an end part of the gap (37) in an axial direction of the rotation axis is provided.

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