Abstract:
The present disclosure provides a membrane separation method of a cell suspension which can appropriately separate cells from debris, and a cell culture device. That is, membrane separation processing of the cell suspension is performed using a filtration membrane which includes an inlet-side opening formed on one surface and an outlet-side opening, which is formed on the other surface and communicates with the inlet-side opening, and in which the inlet-side opening and the outlet-side opening are disposed at positions deviated in a direction parallel to the surfaces of the membrane.
Abstract:
Provided are a white polyester film including a polyester and white particles, in which, at an equivalent of a thickness of 250 μm, a machine stretching direction tear strength FMD is 2.5 to 6.0 N, a transverse stretching direction tear strength FTD is 2.0 to 5.0 N, a ratio of the machine stretching direction tear strength FMD to the transverse stretching direction tear strength FTD is 1.05 to 4.00, and a concentration of terminal carboxyl groups is 5 to 25 equivalents/ton, a method for manufacturing the same, a solar cell back sheet and a solar cell module in which the same white polyester film is used.
Abstract:
An object of the present invention is to provide a method for selecting cells, an apparatus for selecting cells, and an apparatus system for selecting cells, which enables evaluation of a damage resistance of the cells in a suspension culture system. According to the present invention, provided is a method for selecting cells including introducing cells into a micro flow passage and allowing the cells to pass through the micro flow passage, evaluating a damage resistance of the cells to damage received by the cells due to passing through the micro flow passage, and selecting the cells based on the evaluation of the damage resistance.
Abstract:
A method for producing a product related to the specified technology includes adjusting the concentration of cells in a culture vessel to a value of from 3×107 cells/ml to 3×108 cells/ml; in a case in which the average diameter of single cells in the culture vessel is designated as A, adjusting the number proportion of cells having a single cell diameter of 1.4×A or greater in the culture vessel to 5% or less, and adjusting the number proportion of cells having a single cell diameter in the range of A±A/7 to 50% or more.
Abstract:
An object of the present invention is to provide a method that makes it possible to suppress membrane clogging in a production method for a product by cell culture, and a product that is produced by the method. According to the present invention, there is provided a production method for a product, which includes culturing cells, where the production method for a product is perfusion culture in which a cell density at a time of production of the product is 80×106 cells/mL or more and 300×106 cells/mL or less, where a period of the perfusion culture is 13 days or more and 500 days or less, and in a period of at least one day or more in the period of the perfusion culture, X [mol/L/day], which is an alkali addition rate per day, is controlled in a range of 0≤X
Abstract:
In the cell culture device, a volume of a space defined by a diaphragm of the diaphragm pump and a plane at an outer edge of the diaphragm is 1 cm3 or more and 20 cm3 or less, in a case where an angle formed by a straight line connecting a foot of a perpendicular line from the apex to the plane to each point in the diaphragm and by the perpendicular line is denoted by an angle A, a thickness H of the diaphragm is within a range of 0.5 mm or more and 1.5 mm or less where the angle A is from 0° to 75°, and in a case where a thickness of the diaphragm at the apex is denoted by HT, the thickness H satisfies a relationship of 1≤HT/H≤1.75 at any point where the angle A is from 0° to 75°.
Abstract:
The invention provides a polyester film including: a polyester substrate; and a layered portion that is disposed on at least one surface of the polyester substrate and has an elastic modulus of from 5 MPa to 800 MPa, a ratio of a peak intensity at 988 cm−1 that is observed by subjecting a surface of the polyester substrate to infrared spectroscopy to a peak intensity at 1410 cm−1 that is observed by subjecting a surface of the polyester substrate to infrared spectroscopy satisfying the following Inequality (I): 0.010≦(peak intensity at 988 cm−1)/(peak intensity at 1410 cm−1)≦0.040 (I).
Abstract:
An object of the present invention is to provide a method in which the amount of mannose 5 in a product can be reduced in the production method for the product by cell culture, and a product that is produced by the method. According to the present invention, there is provided a production method for a product, including culturing cells in a culture container, in which the culturing is a perfusion culture method, Expressions (1) to (5) are satisfied in a production period of a product after reaching a target viable cell density, a variation of VCD during the production period is ±20% or less with respect to an average viable cell density during the production period, and a variation of VCV during the production period is ±20% or less with respect to an average value of the VCV during the production period. 0≤Gln-C≤2.5 Expression (1) 0.5≤Gluc-C≤8.0 Expression (2) 1.0≤NH4-C≤8.0 Expression (3) 50≤VCD≤300 Expression (4) 0.6×105≤VCV≤4.0×105 Expression (5) The meanings of the symbols in the expressions are as defined in the present specification.
Abstract:
Provided is a cell culture apparatus including a culture vessel that stores a cell suspension containing cells; a first filter part that has a first filter membrane that performs membrane separation treatment on the cell suspension extracted from the culture vessel; a first circulation flow path that allows components blocked by the first filter membrane to return to the culture vessel; a second filter part that has a second filter membrane that performs membrane separation treatment on components of the cell suspension permeated through the first filter membrane; a second circulation flow path that allows components permeated through the second filter membrane to return to the culture vessel; and a recovery flow path that recovers components blocked by the second filter membrane. In the cell culture apparatus, an average hole diameter of the first filter membrane is 20 µm or smaller, and 0
Abstract:
A cell culture device (1) includes: a culture vessel (10 ) that accommodates a cell suspension containing cells and a culture medium; and a stirring device (30) that is provided at a bottom part of the culture vessel (10) to stir the cell suspension accommodated in the culture vessel (10). The stirring device (30) has a shaft part (32), and a rotating part (33) rotatable with the shaft part (32) as a rotation axis. The rotating part (33) has a hole part (36) into which the shaft part (32) is inserted, and a gap (37) of 100 μm or more is secured between an interior wall demarcating the hole part (36) and the shaft part (32). A closing part (38) that closes an end part of the gap (37) in an axial direction of the rotation axis is provided.