公开(公告)号：US11613734B2

公开(公告)日：2023-03-28

申请号：US17619128

申请日：2021-06-11

申请人： FULLSTEM CO., LTD.

发明人： Shunmei Chiba

IPC分类号： C12N5/0775

摘要： A method of efficiently recovering a large amount of exosomes from mesenchymal stem cells is provided. The method includes: a three dimensional culture step of three dimensionally culturing mesenchymal stem cells in a medium containing sugar by using a nonwoven fabric as a scaffold; a post-plateau culture step of further culturing for a certain period of time after the amount of the sugar consumed by the mesenchymal stem cells reaches a plateau; and an exosome recovery step of recovering exosomes from the mesenchymal stem cells. The mesenchymal stem cells are adipose-derived mesenchymal stem cells.

公开(公告)号：US12049641B2

公开(公告)日：2024-07-30

申请号：US16328584

申请日：2017-12-25

申请人： FULLSTEM CO., LTD.

发明人： Shunmei Chiba

IPC分类号： C12N5/0735 ,  C12N5/074 ,  C12N5/0789

CPC分类号： C12N5/0606 ,  C12N5/0647 ,  C12N5/0696 ,  C12N2509/10 ,  C12N2527/00

摘要： Provided is a method for efficiently harvesting cells cultured at a large scale from a three-dimensional porous scaffold. This method includes: a culturing step in which cells are cultured on a three-dimensional porous scaffold sealed in a culture vessel; an enzymatic treatment step in which an enzyme solution containing trypsin-EDTA, caseinase, and/or collagenase is added to the culture vessel to conduct an enzymatic treatment on the cultured cells in the culture vessel; and a vibration treatment step in which a vibration treatment is conducted, i.e., a base that makes a circular motion within a horizontal plane and a bottom surface of the culture vessel are made to come into surface contact with one another so as to impart a vertical-direction vibration to the cultured cells that have undergone the enzymatic treatment.

公开(公告)号：US11661575B2

公开(公告)日：2023-05-30

申请号：US16632765

申请日：2019-02-14

申请人： FULLSTEM CO., LTD.

发明人： Shunmei Chiba ,  Young-Jin Lee ,  Takaki Ushifusa

IPC分类号： C12M1/12 ,  C12M3/06 ,  C12M1/00 ,  C12N5/0775

CPC分类号： C12M25/14 ,  C12M27/16 ,  C12M27/22 ,  C12N5/0663 ,  C12N5/0667 ,  C12N5/0668

摘要： Provided is a cell culture device that has a simple structure and ensures sufficient nutrient supply to cells and oxygenation of the cells to thereby enable mass cell culture. The cell culture device comprises: a cell culture container which is an approximately cylindrical body provided with a flat bottom part at the lower end; a dish-shaped body having an approximately disc shape which is provided with a plurality of magnetic attraction members, said magnetic attraction members being positioned in the circumferential part at equal intervals, and horizontally disposed in a non-contact state within a hollow space inside the cell culture container; and a cyclic body which is provided with a plurality of magnetic attraction members and positioned outside the cell culture container so that the cell culture container is located within the cycle thereof. The cyclic body and the dish-shaped body move vertically in magnetic conjunction to thereby agitate a medium and supply nutrients to cells.