公开(公告)号：US5395856A

公开(公告)日：1995-03-07

申请号：US111686

申请日：1993-08-25

Applicant: Ferdinand C. Haase

Inventor： Ferdinand C. Haase

IPC: B01D15/08 ,  B01J20/32 ,  C08J5/20

CPC classification number: B01D15/3823 ,  B01J20/261 ,  B01J20/264 ,  B01J20/28004 ,  B01J20/286 ,  B01J20/321 ,  B01J20/3217 ,  B01J20/3219 ,  B01J20/3242 ,  B01J20/3248 ,  B01J20/3251 ,  B01J20/3274 ,  B01J20/3425 ,  B01J20/345 ,  B01J2220/54 ,  B01J2220/58 ,  Y10S530/816

Abstract: Novel, improved ligand-containing media, a method of preparation and use in the production of peptides, proteins, and the like, by chromatographic separation, and more specifically media having permanently attached via a covalent bond to an inert solid substrate an avidin polypeptide ligand in the dissociated renatured form which reversibly binds to certain molecules such as proteins, peptide, nucleotides, oligonucleotides, and the like and to other molecules which bind to avidin via biotinylation or by way of their secondary/tertiary micromolecular structures.

Abstract translation: 新颖的，改进的配体介质，通过色谱分离制备和用于制备肽，蛋白质等的方法，更具体地说，通过共价键与惰性固体底物永久连接的亲和素多肽配体 以与蛋白质，肽，核苷酸，寡核苷酸等某些分子可逆结合的解离的复性形式以及通过生物素化或通过其二级/三级小分子结构与抗生物素蛋白结合的其它分子。

公开(公告)号：US5276062A

公开(公告)日：1994-01-04

申请号：US702433

申请日：1991-05-16

Applicant: Ferdinand C. Haase

Inventor： Ferdinand C. Haase

IPC: B01J20/281 ,  B01D15/08 ,  B01J20/26 ,  B01J20/32 ,  C07K1/00 ,  C07K1/16 ,  C07K1/22 ,  C07K17/08 ,  C12N9/00 ,  C12P21/00 ,  C12R1/19 ,  G01N30/88 ,  C08J5/20

CPC classification number: B01J20/286 ,  B01D15/3823 ,  B01J20/321 ,  B01J20/3219 ,  B01J20/3274 ,  Y10S530/816

Abstract: Novel, improved ligand-containing media, a method of preparation and use in the production of peptides, proteins, and the like, by chromatographic separation, and more specifically media having permanently attached via a covalent bond to an inert solid substrate an avidin polypeptide ligand in the dissociated renatured form which reversibly binds to certain molecules such as proteins, peptide, nucleotides, oligonucleotides, and the like and to other molecules which bind to avidin via biotinylation or by way of their secondary/tertiary micromolecular structures.

Abstract translation: 新颖的，改进的配体介质，通过色谱分离制备和用于制备肽，蛋白质等的方法，更具体地说，通过共价键与惰性固体底物永久连接的亲和素多肽配体 以与蛋白质，肽，核苷酸，寡核苷酸等某些分子可逆结合的解离的复性形式以及通过生物素化或通过其二级/三级小分子结构与抗生物素蛋白结合的其它分子。