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    Process for manufacturing morpholino-nucleotides, and use thereof for the analysis of and labelling of nucleic acid sequences
    1.
    发明授权
    Process for manufacturing morpholino-nucleotides, and use thereof for the analysis of and labelling of nucleic acid sequences 失效
    制备吗啉代核苷酸的方法及其用于核酸序列分析和标记的用途

    公开(公告)号：US07081526B2

    公开(公告)日：2006-07-25

    申请号：US10731811

    申请日：2003-12-09

    申请人： Florence Marciacq ,  Sylvie Sauvaigo ,  Jean-François Mouret ,  Jean-Paul Issartel ,  Didier Molko

    发明人： Florence Marciacq ,  Sylvie Sauvaigo ,  Jean-François Mouret ,  Jean-Paul Issartel ,  Didier Molko

    IPC分类号： C07H19/00 ,  C07H21/00

    CPC分类号： C07H19/10 ,  C07H19/00 ,  C07H19/20 ,  C12P19/34

    摘要： The invention relates to the use of morpholino-nucleosides of formula: in which R1 represents a nucleic base and R2 represents a group corresponding to one of the following formulae: —(CH2)n—NH2 —(CH2)n—SH —(CH2)n—COOH —(CH2)n—OH —(CH2)n—NH—R3 —(CH2)n—SR3 —(CH2)n—CO—R3 —(CH2)n—OR3 in which n is an interger ranging from 1 to 12 and R3 is a group derived from a label, from a protein, from an enzyme, from a fatty acid or from a peptide, as chain terminators in a process of DNA or RNA sequencing by the Sanger method, or for the labelling of DNA or RNA fragments.

    摘要翻译： 本发明涉及下式的吗啉代核苷的用途：其中R 1表示核酸，R 2表示对应于下式之一的基团： ？in-line-formula description =“In-line Formulas”end =“lead”？> - （CH 2） n 2 - （CH 2） n -SH <？in-line-formula description =“In-line Formulas”end =“tail”？> <？in-line -formulae description =“In-line Formulas”end =“lead”？ - （CH 2） ）线性公式描述=“在线公式”end =“tail”？> <？in-line-formula description =“In-line Formulas”end = - （CH 2）n - -NH-R 3 - （CH 2） “in-line-formula description =”In-line Formulas“end =”tail“？> <？in-line-formula description =”In - （CH 2）n - （CH 2）n - （CH 2） - （CH 2） ＆lt; / SUB＆gt;＆lt; n＆lt; 3＆lt; 3＆lt; 3＆lt;＆lt;＆lt; in-line-formula description = 其中n为1至12的整数，R 3为来自标记的基团，来自蛋白质，酶，来自 脂肪酸或肽，作为通过Sanger方法进行DNA或RNA测序的方法中的链终止子，或用于标记DNA或RNA片段。

    Process for manufacturing morpholino-nucleotides, and use thereof for the analysis of and labelling of nucleic acid sequences
    3.
    发明授权
    Process for manufacturing morpholino-nucleotides, and use thereof for the analysis of and labelling of nucleic acid sequences 失效
    制备吗啉代核苷酸的方法及其用于核酸序列分析和标记的用途

    公开(公告)号：US07314928B2

    公开(公告)日：2008-01-01

    申请号：US11411505

    申请日：2006-04-26

    申请人： Florence Marciacq ,  Sylvie Sauvaigo ,  Jean-François Mouret ,  Jean-Paul Issartel ,  Didier Molko

    发明人： Florence Marciacq ,  Sylvie Sauvaigo ,  Jean-François Mouret ,  Jean-Paul Issartel ,  Didier Molko

    IPC分类号： C07H21/00 ,  C07H21/04

    CPC分类号： C07H19/10 ,  C07H19/00 ,  C07H19/20 ,  C12P19/34

    摘要： The invention relates to the use of morpholino-nucleosides of formula: in which R1 represents a nucleic base and R2 represents a group corresponding to one of the following formulae: —(CH2)n—NH2 —(CH2)n—SH —(CH2)n—COOH —(CH2)n—OH —(CH2)n—NH—R3 —(CH2)n—SR3 —(CH2)n—CO—R3 —(CH2)n—OR3 in which n is an interger ranging from 1 to 12 and R3 is a group derived from a label, from a protein, from an enzyme, from a fatty acid or from a peptide, as chain terminators in a process of DNA or RNA sequencing by the Sanger method, or for the labelling of DNA or RNA fragments.

    摘要翻译： 本发明涉及下式的吗啉代核苷的用途：其中R 1表示核酸，R 2表示对应于下式之一的基团： ？in-line-formula description =“In-line Formulas”end =“lead”？> - （CH 2） n 2 - （CH 2） n -SH <？in-line-formula description =“In-line Formulas”end =“tail”？> <？in-line -formulae description =“In-line Formulas”end =“lead”？ - （CH 2） ）线性公式描述=“在线公式”end =“tail”？> <？in-line-formula description =“In-line Formulas”end = - （CH 2）n - -NH-R 3 - （CH 2） “in-line-formula description =”In-line Formulas“end =”tail“？> <？in-line-formula description =”In - （CH 2）n - （CH 2）n - （CH 2） - （CH 2） ＆lt; / SUB＆gt;＆lt; 3＆lt; 3＆lt; 3＆lt; =“在线公式”end =“tail”？>其中n是1至12的整数，R 3是从蛋白质衍生自标签的基团，来自酶 ，从脂肪酸或肽中作为链终止子，通过Sanger方法进行DNA或RNA测序，或用于标记DNA或RNA片段。

    Process for manufacturing morpholino-nucleotides, and use thereof for the analysis of and labelling of nucleic acid sequences
    5.
    发明申请
    Process for manufacturing morpholino-nucleotides, and use thereof for the analysis of and labelling of nucleic acid sequences 失效
    制备吗啉代核苷酸的方法及其用于核酸序列分析和标记的用途

    公开(公告)号：US20060183204A1

    公开(公告)日：2006-08-17

    申请号：US11411505

    申请日：2006-04-26

    申请人： Florence Marciacq ,  Sylvie Sauvaigo ,  Jean-Francois Mouret ,  Jean-Paul Issartel ,  Didier Molko

    发明人： Florence Marciacq ,  Sylvie Sauvaigo ,  Jean-Francois Mouret ,  Jean-Paul Issartel ,  Didier Molko

    IPC分类号： C12P19/34 ,  C07F9/6533

    CPC分类号： C07H19/10 ,  C07H19/00 ,  C07H19/20 ,  C12P19/34

    摘要： The invention relates to the use of morpholino-nucleosides of formula: in which R1 represents a nucleic base and R2 represents a group corresponding to one of the following formulae: —(CH2)n—NH2 —(CH2)n—SH —(CH2)n—COOH —(CH2)n—OH —(CH2)n—NH—R3 —(CH2)n—SR3 —(CH2)n—CO—R3 —(CH2)n—OR3 in which n is an interger ranging from 1 to 12 and R3 is a group derived from a label, from a protein, from an enzyme, from a fatty acid or from a peptide, as chain terminators in a process of DNA or RNA sequencing by the Sanger method, or for the labelling of DNA or RNA fragments.

    摘要翻译： 本发明涉及下式的吗啉代核苷的用途：其中R 1表示核酸，R 2表示对应于下式之一的基团： ？in-line-formula description =“In-line Formulas”end =“lead”？> - （CH 2） n 2 - （CH 2） n -SH <？in-line-formula description =“In-line Formulas”end =“tail”？> <？in-line -formulae description =“In-line Formulas”end =“lead”？ - （CH 2） ）线性公式描述=“在线公式”end =“tail”？> <？in-line-formula description =“In-line Formulas”end = - （CH 2）n - -NH-R 3 - （CH 2） “in-line-formula description =”In-line Formulas“end =”tail“？> <？in-line-formula description =”In - （CH 2）n - （CH 2）n - （CH 2） - （CH 2） ＆lt; / SUB＆gt;＆lt; 3＆lt; 3＆lt; 3＆lt; on =“In-line Formulas”end =“tail”？>其中n为1至12的整数，R 3为衍生自标签的组，蛋白质为 酶，从脂肪酸或肽，作为链终止子，通过Sanger方法进行DNA或RNA测序，或用于标记DNA或RNA片段。

    Method for the quantitative assessment of global and specific dna repair capacities of at least one biological medium, and the applications thereof
    8.
    发明申请
    Method for the quantitative assessment of global and specific dna repair capacities of at least one biological medium, and the applications thereof 审中-公开
    用于定量评估至少一种生物培养基的全局和特异性dna修复能力的方法及其应用

    公开(公告)号：US20060147929A1

    公开(公告)日：2006-07-06

    申请号：US10539769

    申请日：2003-12-19

    申请人： Sylvie Sauvaigo

    发明人： Sylvie Sauvaigo

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/68

    摘要： The invention relates to a method for the quantitative assessment of the global and specific DNA repair capacities of a biological medium, consisting in assessing the excision/resynthesis capacities of said medium, and to the applications thereof. The inventive method comprises the following steps: (a) a range of plasmids, each comprising distinct DNA lesions, is prepared; (b) the lesions present on each plasmid from the range of plasmids are characterised; (c) different plasmids from the range and at least one supercoiled control plasmid are deposited on a single solid support, using a pre-established configuration A1 in order to form a functionalised support which is divided into different zones A1 to Ax, x corresponding to an integer which is equal to the number a of biological media to be tested simultaneously, each of zones A1 to Ax comprising said range of plasmids; (d) the functionalised support obtained in step (c) is incubated with different repair solutions; (e) said functionalised support is washed at least once; (f) the signal produced by the marker incorporated into the DNA during the repair reaction in step (d) is measured directly or indirectly; (g) the signal corresponding to each plasmid deposit is recorded and quantified; and (h) the ratio of the signals from plasmids comprising lesions in relation to the control plasmid deposited with same is determined.

    摘要翻译： 本发明涉及一种用于定量评估生物培养基的全面和特异性DNA修复能力的方法，其包括评估所述培养基的切除/再合成能力及其应用。 本发明方法包括以下步骤：（a）制备各自包含不同DNA损伤的质粒范围; （b）表征来自质粒范围的每个质粒上存在的损伤; （c）使用预先确定的构型A 1将与所述范围不同的质粒和至少一个超螺旋对照质粒沉积在单个固体支持物上，以便形成分为不同的功能化载体 区域A 1到A x x，x对应于等于要同时测试的生物介质的数量a的整数，每个区域A 1 < / SUB>包含所述质粒范围的A （d）将步骤（c）中获得的官能化载体与不同的修复溶液一起温育; （e）将所述官能化载体洗涤至少一次; （f）在步骤（d）的修复反应期间由掺入DNA的标记产生的信号直接或间接测量; （g）记录并定量对应于每个质粒沉积物的信号; 和（h）确定包含损伤的质粒相关于与其沉积的对照质粒相关的信号的比例。

    System for detecting nucleic acid hybridization, preparation method and application thereof
    10.
    发明授权
    System for detecting nucleic acid hybridization, preparation method and application thereof 失效
    核酸杂交检测系统及其制备方法及应用

    公开(公告)号：US06210932B1

    公开(公告)日：2001-04-03

    申请号：US09402764

    申请日：1999-12-03

    申请人： Robert Teoule ,  Sylvie Sauvaigo ,  Hervé Bazin

    发明人： Robert Teoule ,  Sylvie Sauvaigo ,  Hervé Bazin

    IPC分类号： C12P1934

    CPC分类号： C12Q1/6816 ,  Y10T436/143333 ,  C12Q2565/519 ,  C12Q2563/107 ,  C12Q2525/186

    摘要： The invention concerns nucleic acid detection using a probe consisting of: a binding domain A, consisting of a single strand polynucleotide; and intermediate domain B, consisting of at least a stop synthon; and a display domain C, consisting of a double strand polynucleotide, wherein is incorporated at least a marked nucleotide constituting detection means. The use of said probes amplifies the hybridisation signal with nucleic acid target sequences.

    摘要翻译： 本发明涉及使用由以下组成的探针的核酸检测：由单链多核苷酸组成的结合结构域A; 和由至少一个停止合成子组成的中间结构域B; 和由双链多核苷酸组成的显示结构域C，其中至少掺入构成检测手段的标记的核苷酸。 所述探针的使用用核酸靶序列扩增杂交信号。