公开(公告)号：US09464119B2

公开(公告)日：2016-10-11

申请号：US13203442

申请日：2010-03-04

申请人： Florian Sonntag ,  Juergen Kleinschmidt ,  Markus Hoerer ,  Kerstin Lux

发明人： Florian Sonntag ,  Juergen Kleinschmidt ,  Markus Hoerer ,  Kerstin Lux

IPC分类号： C12N7/00 ,  A61K39/23 ,  C07K14/005 ,  A61K39/00

CPC分类号： C07K14/005 ,  A61K39/0005 ,  A61K2039/5256 ,  A61K2039/5258 ,  C12N7/00 ,  C12N2750/14122 ,  C12N2750/14123 ,  C12N2750/14143

摘要： The present invention relates to nucleic acids encoding the novel parvoviral protein “assembly activating protein” (AAP), the encoded polypeptides, methods of producing the polypeptides, antibodies specific for AAP, the use of the nucleic acids for the preparation of the polypeptides, the use of the nucleic acids or the polypeptides for the preparation of the parvoviral particle and methods of producing parvoviral particles essentially consisting of VP3. Furthermore, the invention relates to parvoviral particles essentially consisting of VP3 and/or obtainable by the above method as well as expression cassettes comprising (i) a heterologous promoter and (ii) VP3 coding sequence and/or fragment Z. The invention further relates to a medicament, particularly a vaccine, comprising the parvoviral particles or expression cassettes and their use.

摘要翻译： 本发明涉及编码新型细小病毒蛋白“装配激活蛋白”（AAP）的核酸，编码多肽，产生多肽的方法，对AAP特异的抗体，使用该核酸来制备多肽， 使用核酸或多肽来制备细小病毒颗粒以及生产基本上由VP3组成的细小病毒颗粒的方法。 此外，本发明涉及基本由VP3组成和/或可通过上述方法获得的细小病毒颗粒以及包含（i）异源启动子和（ii）VP3编码序列和/或片段Z的表达盒。本发明还涉及 包括细小病毒颗粒或表达盒的药物，特别是疫苗及其用途。

公开(公告)号：US20120135022A1

公开(公告)日：2012-05-31

申请号：US13203442

申请日：2010-03-04

申请人： Florian Sonntag ,  Juergen Kleinschmidt ,  Markus Hoerer ,  Kerstin Lux

发明人： Florian Sonntag ,  Juergen Kleinschmidt ,  Markus Hoerer ,  Kerstin Lux

IPC分类号： A61K35/76 ,  C07K14/015 ,  C07K16/08 ,  C12N7/00 ,  A61K39/23 ,  A61P37/02 ,  A61P35/00 ,  A61P37/08 ,  A61P3/00 ,  A61P29/00 ,  A61P25/00 ,  A61P27/02 ,  A61P25/30 ,  A61P1/00 ,  A61P19/02 ,  C12N15/35

CPC分类号： C07K14/005 ,  A61K39/0005 ,  A61K2039/5256 ,  A61K2039/5258 ,  C12N7/00 ,  C12N2750/14122 ,  C12N2750/14123 ,  C12N2750/14143

摘要： The present invention relates to nucleic acids encoding the novel parvoviral protein “assembly activating protein” (AAP), the encoded polypeptides, methods of producing the polypeptides, antibodies specific for AAP, the use of the nucleic acids for the preparation of the polypeptides, the use of the nucleic acids or the polypeptides for the preparation of the parvoviral particle and methods of producing parvoviral particles essentially consisting of VP3 by providing in addition to the coding sequence of the parvoviral structural protein VP3 a sequence fragment Z/a nucleic acid encoding AAP in the cell and expressing VP3 and fragment Z under control of a rep-independent promoter. Furthermore, the present invention relates to parvoviral particles essentially consisting of VP3 and/or obtainable by the above method as well as expression cassettes comprising (i) a heterologous promoter and (ii) VP3 coding sequence and/or fragment Z. The present invention further relates to a medicament, particularly a vaccine, comprising the parvoviral particles or expression cassettes and their use.

摘要翻译： 本发明涉及编码新型细小病毒蛋白“装配激活蛋白”（AAP）的核酸，编码多肽，产生多肽的方法，对AAP特异的抗体，使用该核酸来制备多肽， 使用核酸或多肽进行细小病毒颗粒的制备以及生产由VP3组成的细小病毒颗粒的方法，除了通过提供细小病毒结构蛋白VP3的编码序列之外还提供编码AAP的序列片段Z / a核酸 细胞并在代表性独立启动子的控制下表达VP3和片段Z。 此外，本发明涉及基本由VP3组成和/或可通过上述方法获得的细小病毒颗粒以及包含（i）异源启动子和（ii）VP3编码序列和/或片段Z的表达盒。本发明进一步 涉及包含细小病毒颗粒或表达盒的药物，特别是疫苗及其用途。