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公开(公告)号：US11697825B2
公开(公告)日：2023-07-11
申请号：US15535389
申请日：2015-12-11
发明人： James McLaughlin , Robert Kotin
IPC分类号： C12N15/864 , C12N15/35 , C12N7/00 , C07K14/015 , C12N15/09 , C12N15/861 , C07K14/005 , C12N15/86 , C12N7/02
CPC分类号： C12N15/861 , C07K14/005 , C12N7/00 , C12N7/02 , C12N15/86 , C12N15/864 , C12N2710/14144 , C12N2750/14122 , C12N2750/14143 , C12N2750/14151
摘要： The present invention is directed to viral vectors and methods of their production and use.
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2.发明授权公开(公告)号：US11357867B2
公开(公告)日：2022-06-14
申请号：US17495949
申请日：2021-10-07
IPC分类号： A61K48/00 , C12N15/63 , C12N15/11 , C12N15/35 , C07K14/005 , C12N15/86 , C12N7/00 , C07K14/705 , C12N9/64
摘要： Sequences of novel adeno-associated virus capsids and vectors and host cells containing these sequences are provided. Also described are methods of using such host cells and vectors in production of rAAV particles. AAV-mediated delivery of therapeutic and immunogenic genes using the vectors of the invention is also provided.
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3.发明授权Viral vectors with modified transduction profiles and methods of making and using the same 有权具有修饰的转导概况的病毒载体及其制备和使用方法公开(公告)号：US09409953B2
公开(公告)日：2016-08-09
申请号：US13984840
申请日：2012-02-10
申请人： Aravind Asokan , Nagesh Pulicherla
发明人： Aravind Asokan , Nagesh Pulicherla
IPC分类号： C07K14/005 , C12N15/86 , C12N7/00 , C07K14/015 , C07K14/01 , A61K39/23 , C12N15/35
CPC分类号： C07K14/005 , C12N7/00 , C12N15/86 , C12N2750/14121 , C12N2750/14122 , C12N2750/14132 , C12N2750/14133 , C12N2750/14143 , C12N2750/14145 , C12N2750/14152
摘要： The present invention provides AAV capsid proteins, virus capsids comprising said capsid proteins and virus vectors comprising said capsid proteins, wherein the AAV capsid proteins have one or more mutations, wherein the mutation(s) result in a phenotype of decreased liver transduction and/or reduced glycan binding affinity as compared to a control. The invention also provides methods of administering the virus vectors and virus capsids of the invention to a cell or to a subject.
摘要翻译： 本发明提供AAV衣壳蛋白，包含所述衣壳蛋白的病毒衣壳和包含所述衣壳蛋白的病毒载体，其中所述AAV衣壳蛋白具有一个或多个突变，其中所述突变导致肝转导减少的表型和/或 与对照相比，降低的聚糖结合亲和力。 本发明还提供将本发明的病毒载体和病毒衣壳施用于细胞或受试者的方法。
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公开(公告)号：US06913922B1
公开(公告)日：2005-07-05
申请号：US09573740
申请日：2000-05-18
申请人： Abraham Bout , Menzo Havenga , Ronald Vogels
发明人： Abraham Bout , Menzo Havenga , Ronald Vogels
IPC分类号： A61K48/00 , C12N5/02 , C12N5/10 , C12N7/00 , C12N15/00 , C12N15/09 , C12N15/35 , C12N15/63 , C12N15/70 , C12N15/74 , C12N15/861
CPC分类号： C07K14/005 , C12N7/00 , C12N15/86 , C12N2710/10321 , C12N2710/10322 , C12N2710/10343 , C12N2710/10345 , C12N2710/10352 , C12N2810/6018 , C12N2830/00
摘要： Adenovirus serotypes differ in their natural tropism. The adenovirus serotypes 2, 4, 5, and 7 all have a natural affiliation towards lung epithelia and other respiratory tissues. In contrast, serotypes 40 and 41 have a natural affiliation towards the gastrointestinal tract. The serotypes described, differ in at least capsid proteins (penton-base, hexon), proteins responsible for cell binding (fiber protein), and proteins involved in adenovirus replication. This difference in tropism and capsid protein among serotypes has led to the many research efforts aimed at redirecting the adenovirus tropism by modification of the capsid proteins.
摘要翻译： 腺病毒血清型的自然向性不同。 腺病毒血清型2,4,5和7都具有与肺上皮细胞和其他呼吸组织的天然亲和关系。 相比之下，血清型40和41与胃肠道具有天然的联系。 所描述的血清型至少在衣壳蛋白（penton-base，hexon），负责细胞结合的蛋白质（纤维蛋白）以及涉及腺病毒复制的蛋白质中不同。 血清型中对向性和衣壳蛋白的这种差异导致了许多研究工作，旨在通过修饰衣壳蛋白来重新定向腺病毒向性。
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公开(公告)号：US5932476A
公开(公告)日：1999-08-03
申请号：US480020
申请日：1995-06-07
IPC分类号： A61K39/12 , A61K38/00 , A61K39/00 , A61K39/23 , A61K39/395 , A61K39/42 , A61K48/00 , A61P31/12 , C07H21/00 , C07K1/00 , C07K2/00 , C07K4/00 , C07K5/00 , C07K7/00 , C07K14/00 , C07K14/01 , C07K14/015 , C07K16/00 , C07K16/08 , C07K17/00 , C07K19/00 , C12N1/21 , C12N5/10 , C12N7/00 , C12N7/01 , C12N15/09 , C12N15/11 , C12N15/34 , C12N15/35 , C12N15/63 , C12N15/86 , C12P21/02 , C12P21/08 , C12Q1/68 , C12Q1/70 , G01N33/53 , G01N33/532 , G01N33/561 , G01N33/569 , G01N33/574 , G01N33/577 , C07H21/04 , C12N15/85
CPC分类号： C07K14/005 , C12N15/86 , G01N33/56983 , G01N33/574 , A61K38/00 , A61K39/00 , A61K48/00 , C12N2750/10022 , C12N2750/10043 , C12N2830/00 , C12N2830/008 , C12N2830/60 , Y10S424/816 , Y10S435/81
摘要： Recombinant genetic information (DNA or RNA), comprising a Chicken Anemia Virus (CAV)-specific nucleotide sequence and the use thereof for diagnostics, vaccination or protein production are described. Recombinant CAV protein and the use thereof for diagnostics, vaccination or production of CAV-specific antibodies, and use of CAV-specific antibodies thus obtained, are also described. The cloned complete CAV DNA genome disclosed is representative of CAV in the field, worldwide. By means of PCR with primers derived from the cloned CAV genome, CAV-specific sequences corresponding with or complementary to the nucleotide sequence described can be detected. CAV harbors a specific promoter/enhancer element which regulates the CAV transcriptional activity. Based upon this, a sensitive CAV-specific PCR and a method for validating negative PCR results and for estimating the number of CAV DNA copies present in the analyzed sample have been developed.
摘要翻译： 描述了包含鸡贫血病毒（CAV）特异性核苷酸序列的重组遗传信息（DNA或RNA）及其用于诊断，疫苗接种或蛋白质生产的用途。 还描述了重组CAV蛋白及其用于诊断，接种或产生CAV特异性抗体的用途，以及使用由此获得的CAV特异性抗体。 公开的克隆的完整的CAV DNA基因组在世界范围内代表了该领域的CAV。 通过使用来自克隆的CAV基因组的引物进行PCR，可以检测与所述核苷酸序列对应或互补的CAV特异性序列。 CAV具有调节CAV转录活性的特异性启动子/增强子元件。 基于此，开发了敏感的CAV特异性PCR和用于验证阴性PCR结果和估计分析样品中存在的CAV DNA拷贝数的方法。
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公开(公告)号：US5885585A
公开(公告)日：1999-03-23
申请号：US647655
申请日：1996-05-15
IPC分类号： C12N15/09 , A61K39/00 , A61K39/23 , A61P31/12 , C07K14/015 , C12N7/00 , C12N7/04 , C12N15/35 , C12R1/92 , A61K39/12 , C07H21/04
CPC分类号： C07K14/005 , A61K39/00 , C12N2750/14322
摘要： The invention is directed to attenuated canine parvovirus (CPV) strains which may be used as a veterinary vaccine against CPV disease. The invention is further directed to a virus stock generated from a genomic DNA clone of the attenuated CPV strain which is used as a veterinary vaccine and is able to confer protective immunity to dogs against challenge with virulent CPV. Methods are given for the production of an attenuated CPV virus from a cloned CPV genome which may be used as a veterinary vaccine.
摘要翻译： 本发明涉及可用作针对CPV疾病的兽用疫苗的减毒犬细小病毒（CPV）菌株。 本发明进一步涉及从减毒CPV毒株的基因组DNA克隆产生的病毒原液，其被用作兽医疫苗并且能够赋予狗免受针对强毒性CPV攻击的保护性免疫。 给出了可用作兽医疫苗的克隆CPV基因组产生减毒CPV病毒的方法。
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7.发明授权D-amino acid oxidase of F. solani and methods for its recombinant production 失效F. solani的D-氨基酸氧化酶及其重组生产方法
公开(公告)号：US5773272A
公开(公告)日：1998-06-30
申请号：US536277
申请日：1995-09-29
申请人： Takao Isogai , Hiroki Ono , Hitoshi Kojo
发明人： Takao Isogai , Hiroki Ono , Hitoshi Kojo
CPC分类号： C12N9/0024 , C12P35/00
摘要： D-amino acid oxidase (DAO) originated from Fusarium solani M-0718, a DNA compound encoding the oxidase, expression vectors containing said DNA compound are provided. Esherichia coli transformants capable of producing recombinant DAO in high level are also provided.
摘要翻译： 提供源自茄病菌M-0718的D-氨基酸氧化酶（DAO），其是编码氧化酶的DNA化合物，含有所述DNA化合物的表达载体。 还提供了能够生产高水平重组DAO的大肠杆菌转化体。
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公开(公告)号：US5495071A
公开(公告)日：1996-02-27
申请号：US72281
申请日：1993-06-04
IPC分类号： A01H1/00 , A01H5/00 , C07K14/325 , C07K14/37 , C12N1/21 , C12N5/10 , C12N15/09 , C12N15/32 , C12N15/35 , C12N15/78 , C12N15/82 , C12P21/02 , C12R1/07 , A01H4/00
CPC分类号： C12N15/78 , C07K14/325 , C12N15/8286
摘要： A method for producing genetically transformed plants exhibiting toxicity to Coleopteran insects is disclosed. In another aspect, the present invention embraces chimeric plant genes, genetically transformed cells and differentiated plants which exhibit toxicity to Coleopteran insects. In yet another aspect, the present invention embraces bacterial cells and plant transformation vectors comprising a chimeric plant gene encoding a Coleopteran toxin protein of Bacillus thuringiensis.
摘要翻译： 公开了一种生产对鞘翅目昆虫具有毒性的遗传转化植物的方法。 另一方面，本发明包括嵌合植物基因，遗传转化细胞和分化植物，其对鞘翅目昆虫具有毒性。 另一方面，本发明包括细菌细胞和植物转化载体，其包含编码苏云金芽孢杆菌的鞘翅目毒素蛋白质的嵌合植物基因。
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公开(公告)号：US4971793A
公开(公告)日：1990-11-20
申请号：US191684
申请日：1988-05-09
申请人： Harry A. Wood , Colin R. Parrish
发明人： Harry A. Wood , Colin R. Parrish
IPC分类号： A61K39/00 , C07K14/015 , C12N15/35 , C12N15/866
CPC分类号： C12N15/86 , C07K14/005 , A61K39/00 , C12N2710/14143 , C12N2750/14322 , Y10S424/818
摘要： A recombinant subunit vaccine for protecting dogs against infection caused by canine parvovirus comprising VP-2 protein produced during replication of a recombinant baculovirus in insect tissue culture cells or insects which are a permissive host for the replication of selected baculoviruses.
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公开(公告)号：US09228174B2
公开(公告)日：2016-01-05
申请号：US13583920
申请日：2011-03-11
CPC分类号： C12N7/00 , C07K14/005 , C12N15/86 , C12N2710/14143 , C12N2750/14143 , C12N2750/14151 , C12N2750/14152 , C12N2800/105 , C12N2800/50 , C12N2800/90 , C12N2820/00
摘要： Nucleic acids encoding Parvoviral Rep proteins with a mutated nuclear localization signal (NLS) are provided. Also provided is a nucleic acid comprising a nucleotide sequence encoding a Parvoviral Rep protein with a mutated zinc finger domain and a nucleic acid comprising a nucleotide sequence encoding a Parvoviral Rep protein comprising an amino acid mutation at position 43, 57, 79, 97, 120, 179, 305, 484, 493 or 571 with reference to SEQ ID NO: 2. Nucleic acid constructs and cells, such as insect cells, comprising the nucleic acids are provided as well as a method for producing a recombinant Parvoviral virion using the nucleic acids.
摘要翻译： 提供了编码具有突变核定位信号（NLS）的细小病毒Rep蛋白的核酸。 还提供了包含编码具有突变的锌指结构域的细小病毒Rep蛋白的核苷酸序列的核酸和包含编码包含第43,57,79,97,120的氨基酸突变的细小病毒Rep蛋白的核苷酸序列的核酸 ，179,305,448,493或571，参考SEQ ID NO：2.提供了核酸构建体和包含核酸的细胞，例如昆虫细胞，以及使用核酸产生重组细小病毒病毒体的方法 酸。
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