公开(公告)号：US10669574B2

公开(公告)日：2020-06-02

申请号：US15324218

申请日：2015-07-10

申请人： Fluoresentric, Inc.

发明人： Brian Caplin ,  Bryson Green

IPC分类号： C12Q1/6853 ,  C12Q1/6811 ,  C12Q1/689 ,  C12Q1/686

摘要： Methods and reagents suitable for conducing polymerase chain reaction are described. In particular, a nucleic acid amplification design strategy and thermal cycling profile to enable efficient amplification of multiple nucleic acid targets along with improved sensitivity is disclosed. The present disclosure also describes methods and devices for increasing the melting temperature (Tm) of a primer.

公开(公告)号：US20150099659A1

公开(公告)日：2015-04-09

申请号：US14510939

申请日：2014-10-09

申请人： FLUORESENTRIC, INC

发明人： Brian Caplin

IPC分类号： C12Q1/68

CPC分类号： C12Q1/686 ,  C12Q1/6818 ,  C12Q2525/161 ,  C12Q2525/301 ,  C12Q2527/107 ,  C12Q2537/119 ,  C12Q2565/101 ,  C12Q2565/1015

摘要： Methods and reagents suitable for conducing polymerase chain reaction are described. In particular, the disclosure provides probes and primers that are suitable in dynamic flux amplification procedures. In aspects, the disclosure provides long oligonucleotide probes and primers, as well as triplex forming probes and primers, which function within the narrow Tm ranges used with dynamic flux amplification. However, embodiments are also provided wherein the probes and primers taught herein can be utilized in standard PCR.

摘要翻译： 描述适用于引发聚合酶链反应的方法和试剂。 特别地，本公开提供了适用于动态通量扩增程序的探针和引物。 在一些方面，本公开提供长的寡核苷酸探针和引物，以及三重形成探针和引物，其在用于动态通量扩增的窄Tm范围内起作用。 然而，还提供了其中本文教导的探针和引物可用于标准PCR的实施方案。

公开(公告)号：US10370707B2

公开(公告)日：2019-08-06

申请号：US14510939

申请日：2014-10-09

申请人： Fluoresentric, Inc

发明人： Brian Caplin

IPC分类号： C12Q1/68 ,  C07H21/00 ,  C12Q1/686 ,  C12Q1/6818

摘要： Methods and reagents suitable for conducing polymerase chain reaction are described. In particular, the disclosure provides probes and primers that are suitable in dynamic flux amplification procedures. In aspects, the disclosure provides long oligonucleotide probes and primers, as well as triplex forming probes and primers, which function within the narrow Tm ranges used with dynamic flux amplification. However, embodiments are also provided wherein the probes and primers taught herein can be utilized in standard PCR.