公开(公告)号：US20170226576A1

公开(公告)日：2017-08-10

申请号：US15493341

申请日：2017-04-21

申请人： Fluoresentric, Inc.

发明人： Brian Erich Caplin

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6858 ,  C12Q1/6816 ,  C12Q1/6825 ,  C12Q1/6827 ,  C12Q1/6844 ,  C12Q1/6846 ,  C12Q1/6848 ,  C12Q1/6876 ,  C12Q1/6886 ,  C12Q1/689 ,  C12Q1/703 ,  C12Q2600/106 ,  C12Q2600/136 ,  C12Q2600/156 ,  C12Q2527/107 ,  C12Q2527/101

摘要： Provided herein are dynamic flux nucleic acid sequence amplification methods. The dynamic flux nucleic acid sequence amplification methods described herein are capable of amplifying nucleic acid sequences within a narrow temperature range. In some aspects, the disclosure provides for real-time dynamic flux nucleic acid sequence amplification methods.

公开(公告)号：US20170198342A1

公开(公告)日：2017-07-13

申请号：US15324218

申请日：2015-07-10

申请人： Fluoresentric, Inc.

发明人： Brian CAPLIN ,  Brian HICKE ,  Bryson GREEN

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6853 ,  C12Q1/6811 ,  C12Q1/686 ,  C12Q1/689 ,  C12Q2600/158 ,  C12Q2527/107

摘要： Methods and reagents suitable for conducing polymerase chain reaction are described. In particular, a nucleic acid amplification design strategy and thermal cycling profile to enable efficient amplification of multiple nucleic acid targets along with improved sensitivity is disclosed. The present disclosure also describes methods and devices for increasing the melting temperature (Tm) of a primer.

公开(公告)号：US09353408B2

公开(公告)日：2016-05-31

申请号：US14825364

申请日：2015-08-13

申请人： Fluoresentric, Inc

发明人： Brian Erich Caplin

IPC分类号： C12Q1/68 ,  C12Q1/70

CPC分类号： C12Q1/6827 ,  C12Q1/6825 ,  C12Q1/6844 ,  C12Q1/6846 ,  C12Q1/6876 ,  C12Q1/6886 ,  C12Q1/689 ,  C12Q1/703 ,  C12Q2600/106 ,  C12Q2600/136 ,  C12Q2600/156 ,  C12Q2527/107 ,  C12Q2527/101

摘要： Provided herein are dynamic flux nucleic acid sequence amplification methods. The dynamic flux nucleic acid sequence amplification methods described herein are capable of amplifying nucleic acid sequences within a narrow temperature range. In some aspects, the disclosure provides for real-time dynamic flux nucleic acid sequence amplification methods.

摘要翻译： 本文提供动态通量核酸序列扩增方法。 本文所述的动态通量核酸序列扩增方法能够在窄的温度范围内扩增核酸序列。 在一些方面，本公开提供了实时动态通量核酸序列扩增方法。

公开(公告)号：US10669574B2

公开(公告)日：2020-06-02

申请号：US15324218

申请日：2015-07-10

申请人： Fluoresentric, Inc.

发明人： Brian Caplin ,  Bryson Green

IPC分类号： C12Q1/6853 ,  C12Q1/6811 ,  C12Q1/689 ,  C12Q1/686

摘要： Methods and reagents suitable for conducing polymerase chain reaction are described. In particular, a nucleic acid amplification design strategy and thermal cycling profile to enable efficient amplification of multiple nucleic acid targets along with improved sensitivity is disclosed. The present disclosure also describes methods and devices for increasing the melting temperature (Tm) of a primer.

公开(公告)号：US20180264476A1

公开(公告)日：2018-09-20

申请号：US15760360

申请日：2016-09-16

申请人： Fluoresentric, Inc.

发明人： Brian CAPLIN ,  Brian HICKE ,  Bryson GREEN

IPC分类号： B01L7/00 ,  C12Q1/686

CPC分类号： B01L7/52 ,  B01L7/525 ,  B01L7/54 ,  B01L2300/025 ,  B01L2300/0663 ,  B01L2300/0854 ,  B01L2300/1805 ,  B01L2300/1822 ,  B01L2400/0445 ,  C12Q1/68 ,  C12Q1/686

摘要： Embodiments of the current disclosure are directed towards apparatus, methods and systems configured for dynamic flux amplification of samples in reaction vessels. In some embodiments, an apparatus comprising a reaction vessel and a heat source is disclosed. The reaction vessel may include a first wall and an opposing second wall positioned so as to define a sample chamber therebetween, a width of the sample chamber being less than about 2 mm. The heat source may be configured to vary a first temperature of the first wall and a second temperature of the second wall such that a temperature difference between the first temperature and the second temperature induces thermal cycling in a solution contained within the sample chamber of the reaction vessel.

公开(公告)号：US09670531B2

公开(公告)日：2017-06-06

申请号：US15138517

申请日：2016-04-26

申请人： Fluoresentric, Inc.

发明人： Brian Erich Caplin

IPC分类号： C12Q1/68 ,  C12Q1/70

CPC分类号： C12Q1/6827 ,  C12Q1/6825 ,  C12Q1/6844 ,  C12Q1/6846 ,  C12Q1/6876 ,  C12Q1/6886 ,  C12Q1/689 ,  C12Q1/703 ,  C12Q2600/106 ,  C12Q2600/136 ,  C12Q2600/156 ,  C12Q2527/107 ,  C12Q2527/101

摘要： Provided herein are dynamic flux nucleic acid sequence amplification methods. The dynamic flux nucleic acid sequence amplification methods described herein are capable of amplifying nucleic acid sequences within a narrow temperature range. In some aspects, the disclosure provides for real-time dynamic flux nucleic acid sequence amplification methods.

公开(公告)号：US20150099659A1

公开(公告)日：2015-04-09

申请号：US14510939

申请日：2014-10-09

申请人： FLUORESENTRIC, INC

发明人： Brian Caplin

IPC分类号： C12Q1/68

CPC分类号： C12Q1/686 ,  C12Q1/6818 ,  C12Q2525/161 ,  C12Q2525/301 ,  C12Q2527/107 ,  C12Q2537/119 ,  C12Q2565/101 ,  C12Q2565/1015

摘要： Methods and reagents suitable for conducing polymerase chain reaction are described. In particular, the disclosure provides probes and primers that are suitable in dynamic flux amplification procedures. In aspects, the disclosure provides long oligonucleotide probes and primers, as well as triplex forming probes and primers, which function within the narrow Tm ranges used with dynamic flux amplification. However, embodiments are also provided wherein the probes and primers taught herein can be utilized in standard PCR.

摘要翻译： 描述适用于引发聚合酶链反应的方法和试剂。 特别地，本公开提供了适用于动态通量扩增程序的探针和引物。 在一些方面，本公开提供长的寡核苷酸探针和引物，以及三重形成探针和引物，其在用于动态通量扩增的窄Tm范围内起作用。 然而，还提供了其中本文教导的探针和引物可用于标准PCR的实施方案。

公开(公告)号：US10370707B2

公开(公告)日：2019-08-06

申请号：US14510939

申请日：2014-10-09

申请人： Fluoresentric, Inc

发明人： Brian Caplin

IPC分类号： C12Q1/68 ,  C07H21/00 ,  C12Q1/686 ,  C12Q1/6818

摘要： Methods and reagents suitable for conducing polymerase chain reaction are described. In particular, the disclosure provides probes and primers that are suitable in dynamic flux amplification procedures. In aspects, the disclosure provides long oligonucleotide probes and primers, as well as triplex forming probes and primers, which function within the narrow Tm ranges used with dynamic flux amplification. However, embodiments are also provided wherein the probes and primers taught herein can be utilized in standard PCR.

公开(公告)号：US10337056B2

公开(公告)日：2019-07-02

申请号：US15493341

申请日：2017-04-21

申请人： Fluoresentric, Inc.

发明人： Brian Erich Caplin

IPC分类号： C12Q1/68 ,  C12Q1/6858 ,  C12Q1/6827 ,  C12Q1/6886 ,  C12Q1/689 ,  C12Q1/70 ,  C12Q1/6844 ,  C12Q1/6825 ,  C12Q1/6876 ,  C12Q1/6816 ,  C12Q1/6848

摘要： Provided herein are dynamic flux nucleic acid sequence amplification methods. The dynamic flux nucleic acid sequence amplification methods described herein are capable of amplifying nucleic acid sequences within a narrow temperature range. In some aspects, the disclosure provides for real-time dynamic flux nucleic acid sequence amplification methods.

公开(公告)号：US20190168219A1

公开(公告)日：2019-06-06

申请号：US16031365

申请日：2018-07-10

申请人： Fluoresentric, Inc.

发明人： Brian CAPLIN ,  Brian HICKE ,  Brandon Doyle LOWE ,  Jamie PURCELL ,  Mark POWELSON ,  Bryson GREEN ,  Cameron ORR ,  Jennifer CAMPBELL ,  Katelyn KELLY ,  David OWEN ,  Michael MCMAHON

IPC分类号： B01L3/00 ,  C12M1/12 ,  C12M3/06 ,  C12M1/00 ,  C12M3/00 ,  C12M1/34

摘要： An apparatus includes a sample holder to hold or receive a sample during use. The apparatus also includes a first chamber operably coupled to the sample holder and configured to receive the sample holder. The first chamber includes at least one extraction reagent for extracting nucleic acid from the sample during use to generate a first treated sample. The first chamber and the sample holder collectively define a common longitudinal axis. The apparatus also includes a second chamber operably coupled to the first chamber and disposed along the longitudinal axis. The second chamber includes at least one oligomer for amplifying the nucleic acid to generate a second treated sample.