公开(公告)号：US07659081B2

公开(公告)日：2010-02-09

申请号：US12236418

申请日：2008-09-23

申请人： Frank Cuttitta ,  Ted H. Elsasser ,  Alfredo Martinez ,  Rubén Pio

发明人： Frank Cuttitta ,  Ted H. Elsasser ,  Alfredo Martinez ,  Rubén Pio

IPC分类号： G01N33/53

CPC分类号： C07K16/18 ,  A61K38/1709 ,  A61K2039/505 ,  G01N33/74 ,  G01N2500/00

摘要： The present invention provides methods for the isolation, identification, and purification of adrenomedullin (AM)-binding proteins. Also, provided are methods for utilizing the purified AM-binding proteins, or functional portions thereof, to diagnose, treat, and monitor AM-related diseases, for example, diseases or disorders associated with abnormally elevated AM levels. In addition, the present invention provides a newly identified complex between AM and a specific AM-binding protein 1 (AMBP-1); which has been isolated and identified herein as factor H (fH). The invention also provides AM/AMBP complexes, particularly AM/FH complexes, and antibodies specifically reactive with this complexes. Further provided are methods for identifying and purifying complexes of AM and an AM binding protein using anti-AM/fH antibodies, and methods for treating conditions such as cancer or diabetes utilizing compositions comprising these antibodies. The present invention additionally provides methods for identifying antagonists agents that inhibit the function of AM, factor H, or the AM/factor H complex. The invention also provides methods for treating conditions such as cancer or diabetes using these antagonist agents.

摘要翻译： 本发明提供了分离，鉴定和纯化肾上腺髓质素（AM）结合蛋白的方法。 此外，提供了利用纯化的AM结合蛋白或其功能部分来诊断，治疗和监测AM相关疾病的方法，例如与异常升高的AM水平相关的疾病或病症。 此外，本发明提供了AM和特异性AM结合蛋白1（AMBP-1）之间新鉴定的复合物; 其在本文中被分离和鉴定为因子H（fH）。 本发明还提供AM / AMBP复合物，特别是AM / FH复合物和与这些复合物特异性反应的抗体。 进一步提供了使用抗AM / fH抗体鉴定和纯化AM和AM结合蛋白的复合物的方法，以及使用包含这些抗体的组合物治疗诸如癌症或糖尿病的病症的方法。 本发明另外提供用于鉴定抑制AM，因子H或AM /因子H复合物的功能的拮抗剂的方法。 本发明还提供了使用这些拮抗剂治疗诸如癌症或糖尿病的病症的方法。

公开(公告)号：US20100098703A1

公开(公告)日：2010-04-22

申请号：US12638747

申请日：2009-12-15

申请人： Frank Cuttitta ,  Ted H. Elsasser ,  Alfredo Martinez ,  Rubén Pío

发明人： Frank Cuttitta ,  Ted H. Elsasser ,  Alfredo Martinez ,  Rubén Pío

IPC分类号： A61K39/395 ,  C07K14/00 ,  A61K38/16 ,  C07K16/00 ,  A61P43/00 ,  G01N33/543 ,  G01N33/53

CPC分类号： C07K16/18 ,  A61K38/1709 ,  A61K2039/505 ,  G01N33/74 ,  G01N2500/00

摘要： The present invention provides methods for the isolation, identification, and purification of adrenomedullin (AM)-binding proteins. Also, provided are methods for utilizing the purified AM-binding proteins, or functional portions thereof, to diagnose, treat, and monitor AM-related diseases, for example, diseases or disorders associated with abnormally elevated AM levels. In addition, the present invention provides a newly identified complex between AM and a specific AM-binding protein 1 (AMBP-1); which has been isolated and identified herein as factor H (fH). The invention also provides AM/AMBP complexes, particularly AM/FH complexes, and antibodies specifically reactive with this complexes. Further provided are methods for identifying and purifying complexes of AM and an AM binding protein using anti-AM/fH antibodies, and methods for treating conditions such as cancer or diabetes utilizing compositions comprising these antibodies. The present invention additionally provides methods for identifying antagonists agents that inhibit the function of AM, factor H, or the AM/factor H complex. The invention also provides methods for treating conditions such as cancer or diabetes using these antagonist agents.

摘要翻译： 本发明提供了分离，鉴定和纯化肾上腺髓质素（AM）结合蛋白的方法。 此外，提供了利用纯化的AM结合蛋白或其功能部分来诊断，治疗和监测AM相关疾病的方法，例如与异常升高的AM水平相关的疾病或病症。 此外，本发明提供了AM和特异性AM结合蛋白1（AMBP-1）之间新鉴定的复合物; 其在本文中被分离和鉴定为因子H（fH）。 本发明还提供AM / AMBP复合物，特别是AM / FH复合物和与该复合物特异性反应的抗体。 进一步提供了使用抗AM / fH抗体鉴定和纯化AM和AM结合蛋白的复合物的方法，以及使用包含这些抗体的组合物治疗诸如癌症或糖尿病的病症的方法。 本发明另外提供用于鉴定抑制AM，因子H或AM /因子H复合物的功能的拮抗剂的方法。 本发明还提供了使用这些拮抗剂治疗诸如癌症或糖尿病的病症的方法。

公开(公告)号：US07993857B2

公开(公告)日：2011-08-09

申请号：US12638747

申请日：2009-12-15

申请人： Frank Cuttitta ,  Ted H. Elsasser ,  Alfredo Martinez ,  Rubén Pío

发明人： Frank Cuttitta ,  Ted H. Elsasser ,  Alfredo Martinez ,  Rubén Pío

IPC分类号： G01N1/00 ,  G01N33/53

CPC分类号： C07K16/18 ,  A61K38/1709 ,  A61K2039/505 ,  G01N33/74 ,  G01N2500/00

摘要： The present invention provides methods for the isolation, identification, and purification of adrenomedullin (AM)-binding proteins. Also, provided are methods for utilizing the purified AM-binding proteins, or functional portions thereof, to diagnose, treat, and monitor AM-related diseases, for example, diseases or disorders associated with abnormally elevated AM levels. In addition, the present invention provides a newly identified complex between AM and a specific AM-binding protein 1 (AMBP-1); which has been isolated and identified herein as factor H (fH). The invention also provides AM/AMBP complexes, particularly AM/FH complexes, and antibodies specifically reactive with this complexes. Further provided are methods for identifying and purifying complexes of AM and an AM binding protein using anti-AM/fH antibodies, and methods for treating conditions such as cancer or diabetes utilizing compositions comprising these antibodies. The present invention additionally provides methods for identifying antagonists agents that inhibit the function of AM, factor H, or the AM/factor H complex. The invention also provides methods for treating conditions such as cancer or diabetes using these antagonist agents.

摘要翻译： 本发明提供了分离，鉴定和纯化肾上腺髓质素（AM）结合蛋白的方法。 此外，提供了利用纯化的AM结合蛋白或其功能部分来诊断，治疗和监测AM相关疾病的方法，例如与异常升高的AM水平相关的疾病或病症。 此外，本发明提供了AM和特异性AM结合蛋白1（AMBP-1）之间新鉴定的复合物; 其在本文中被分离和鉴定为因子H（fH）。 本发明还提供AM / AMBP复合物，特别是AM / FH复合物和与该复合物特异性反应的抗体。 进一步提供了使用抗AM / fH抗体鉴定和纯化AM和AM结合蛋白的复合物的方法，以及使用包含这些抗体的组合物治疗诸如癌症或糖尿病的病症的方法。 本发明另外提供用于鉴定抑制AM，因子H或AM /因子H复合物的功能的拮抗剂的方法。 本发明还提供了使用这些拮抗剂治疗诸如癌症或糖尿病的病症的方法。