公开(公告)号：US09662653B2

公开(公告)日：2017-05-30

申请号：US14846931

申请日：2015-09-07

申请人： Frank Leo Spangler

发明人： Frank Leo Spangler

IPC分类号： C12M1/34 ,  B01L7/00

CPC分类号： B01L7/5255 ,  B01L2300/0809 ,  B01L2300/1805 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2300/1883

摘要： In one embodiment, a thermal array comprises a first heating element and a second heating element, each surrounded by insulators which cover five sides of each heating element, held in position by a cooling block. Two thermal arrays are placed opposite each other creating a conduction channel wherein a sample vessel is transported between the heating elements and cooling block as the reaction requires. Due to the low energy consumption of each array, the thermal array system may function using standard D-cell batteries.

公开(公告)号：US09399219B2

公开(公告)日：2016-07-26

申请号：US12697184

申请日：2010-01-29

申请人： Frank Leo Spangler

发明人： Frank Leo Spangler

IPC分类号： C12M1/34 ,  B01L7/00

CPC分类号： B01L7/5255 ,  B01L2300/0809 ,  B01L2300/1805 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2300/1883

摘要： Traditional thermocyclers are heating devices that change sample temperatures by adding heat energy into a sample block that is usually a large metal block and then extracting that heat energy out of the block in a process called ramping the temperature. Presented herein is a technology that eliminates the large mass sample block and ramping temperatures in a sample block and thus in a sample vessel. This design called a Thermal Array requires a fraction of the energy used to process a sample. In addition, the array allows a much smaller thermal cycler to be designed leading to portability of a device. This technology is designed to allow optimal polymerase chain reactions to be executed.

摘要翻译： 传统的热循环仪是通过将热能加入到通常是大金属块的样品块中，然后在称为升高温度的过程中将热能从块中提取出来来改变样品温度的加热装置。 这里提出的是消除样品块中大样本块和斜坡温度以及因此在样品容器中的技术。 这种称为热阵列的设计需要用于处理样品的能量的一小部分。 此外，该阵列允许设计更小的热循环仪，从而导致器件的便携性。 该技术旨在实现最佳的聚合酶链反应。

公开(公告)号：US20150375230A1

公开(公告)日：2015-12-31

申请号：US14846931

申请日：2015-09-07

申请人： Frank Leo Spangler

发明人： Frank Leo Spangler

IPC分类号： B01L7/00

CPC分类号： B01L7/5255 ,  B01L2300/0809 ,  B01L2300/1805 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2300/1883

摘要： In one embodiment, a thermal array comprises a first heating element and a second heating element, each surrounded by insulators which cover five sides of each heating element, held in position by a cooling block. Two thermal arrays are placed opposite each other creating a conduction channel wherein a sample vessel is transported between the heating elements and cooling block as the reaction requires. Due to the low energy consumption of each array, the thermal array system may function using standard D-cell batteries.

摘要翻译： 在一个实施例中，热阵列包括第一加热元件和第二加热元件，每个加热元件由绝缘体围绕，该绝缘体覆盖每个加热元件的五边，通过冷却块保持在适当位置。 两个热阵列彼此相对放置，产生导电通道，其中样品容器在反应需要时在加热元件和冷却块之间传输。 由于每个阵列的能量消耗低，所以热阵列系统可以使用标准的D型电池来实现。

公开(公告)号：US20100210006A1

公开(公告)日：2010-08-19

申请号：US12697184

申请日：2010-01-29

申请人： Frank Leo Spangler

发明人： Frank Leo Spangler

IPC分类号： C12M1/38 ,  C12M1/00

CPC分类号： B01L7/5255 ,  B01L2300/0809 ,  B01L2300/1805 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2300/1883

摘要： Traditional thermocyclers are heating devices that change sample temperatures by adding heat energy into a sample block that is usually a large metal block and then extracting that heat energy out of the block in a process called ramping the temperature. Presented herein is a technology that eliminates the large mass sample block and ramping temperatures in a sample block and thus in a sample vessel. This design called a Thermal Array requires a fraction of the energy used to process a sample. In addition, the array allows a much smaller thermal cycler to be designed leading to portability of a device. This technology is designed to allow optimal polymerase chain reactions to be executed.

摘要翻译： 传统的热循环仪是通过将热能加入到通常是大金属块的样品块中，然后在称为升高温度的过程中将热能从块中提取出来来改变样品温度的加热装置。 这里提出的是消除样品块中大样本块和斜坡温度以及因此在样品容器中的技术。 这种称为热阵列的设计需要用于处理样品的能量的一小部分。 此外，该阵列允许设计更小的热循环仪，从而导致器件的便携性。 该技术旨在实现最佳的聚合酶链反应。

公开(公告)号：US20220162585A1

公开(公告)日：2022-05-26

申请号：US17456215

申请日：2021-11-23

申请人： Frank Leo Spangler

发明人： Frank Leo Spangler

IPC分类号： C12N15/10 ,  C12Q1/6806 ,  B01L3/00 ,  B01L7/00

摘要： A method of nucleic acid extraction utilizes small magnetizable beads (e.g., iron, cobalt, nickel, etc.) that react to the presence of a magnetic field by participating in a paramagnetic flux. The magnetizable beads clump together in a mass inside of a reaction vessel. The reaction vessel can be different shapes and sizes, but it is typically a cylindrical test tube. A magnet is positioned proximal to the reaction vessel with the beads inside. By rotating the magnet around the tube (or spinning the tube), the beads will follow the magnet around the tube due to the magnetic flux, creating a grinding or macerating effect while mixing the contents. When biological samples are also placed into the reaction vessel with the magnetizable beads, they become homogenized in a solution in the reaction vessel as the magnet moves around the vessel.

公开(公告)号：US20220161264A1

公开(公告)日：2022-05-26

申请号：US17456219

申请日：2021-11-23

申请人： Frank Leo Spangler

发明人： Frank Leo Spangler

IPC分类号： B01L7/00

摘要： A thermal array has a first heating element, a second, transparent heating element, a first insulator, a second insulator, and a cooling block. The transparent heating element is a layer of glass with one or more heating wires cast into, or coupled onto, the glass, or a thermo-resistive coating sputtered onto one side of the glass. The transparent heating element may further have a temperature sensor to monitor the temperature and thereby ensure that the layer of glass is held at a predetermined temperature. The thermal array includes bandpass light filters to be able to detect the exact fluorescence being produced.