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公开(公告)号：US5686301A
公开(公告)日：1997-11-11
申请号：US300628
申请日：1994-09-02
摘要： A cell culture vessel which permits gentle but vigorous intermixing and suspending of the cell culture during rotation. The culture vessel features a cell mixing and suspending device combined with a gas exchange membrane such that ample gas exchange occurs without the need to feed oxygen to the cells. The cell mixing and suspending device comprises a baffle system having at least one, and preferably more than one, mixing elements integral with the gas exchange membrane. Movement of the culture vessel, such as for example by rolling, shaking or swirling, moves the mixing elements such that the cells which are to be cultivated are gently intermixed and stably suspended within the cell culture chamber. Pressure variations within the cell culture vessel are accommodated by pressure equalizing device. The pressure equalizing device preferably comprises pressure sheathing made of gas permeable material positioned to form at least a portion of the outer wall of the supply chamber. The pressure sheathing compresses to accommodate increased pressure within the supply chamber. Moreover, the gas permeable material permits additional gas exchange to occur through the supply chamber. Mixing of the nutrient medium is accomplished during movement of the cell culture vessel by way of the extending pressure finger.
摘要翻译： 一种细胞培养容器，其允许在旋转期间温和但有力地混合和悬浮细胞培养物。 培养容器具有与气体交换膜结合的细胞混合和悬浮装置，使得充分的气体交换发生而不需要向细胞供氧。 电池混合和悬浮装置包括具有与气体交换膜成一体的至少一个，优选多于一个的混合元件的挡板系统。 培养容器的移动，例如通过滚动，摇动或旋转，移动混合元件，使待培养的细胞温和地混合并稳定地悬浮在细胞培养室内。 细胞培养容器内的压力变化由压力均衡装置容纳。 压力平衡装置优选地包括由气体可渗透材料制成的压力护套，其被定位成形成供应室的外壁的至少一部分。 压力护套压缩以适应供应室内的增加的压力。 此外，透气材料允许通过供应室发生额外的气体交换。 营养培养基的混合在细胞培养容器通过延伸压力指的运动期间完成。
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公开(公告)号：US5576211A
公开(公告)日：1996-11-19
申请号：US423575
申请日：1995-04-18
IPC分类号： C12M1/02 , C12M1/04 , C12M1/10 , C12M1/12 , C12M1/24 , C12M3/00 , C12M3/04 , C12M3/06 , C12M1/00
CPC分类号： C12M29/04 , C12M23/24 , C12M23/34 , C12M27/02 , Y10S435/818
摘要： Culture vessels for cell cultures having at least one cell culture chamber containing a cell culture mixture that is separated by a dialysis membrane from a nutrient medium in a nutrient supply chamber. Nutrients are transported through the dialysis membrane into the cell culture chamber and metabolic products are transported out of the cell culture chamber into the nutrient supply chamber. A gas exchange membrane that at least partly delimits the cell culture chamber and that is impermeable to liquids and to microorganisms that could contaminate the cell cultures is provided for feeding the gases required for cell culturing to the culture chamber and for discharging the gases generated during cell culturing. The cell culture chamber can advantageously be configured as a stand-alone module supporting both the dialysis membrane and the gas exchange membrane. The nutrient supply chamber can be configured as a cup-shaped module having an open end releasably interconnectable with the cell culture chamber module at the location of the dialysis membrane, via snap elements.
摘要翻译： 用于细胞培养物的培养容器具有至少一个细胞培养室，该细胞培养室含有通过透析膜从营养供应室中的营养培养基分离的细胞培养物混合物。 营养物质通过透析膜转运到细胞培养室中，代谢产物从细胞培养室输送到营养供应室中。 提供至少部分地限定细胞培养室并且对液体和可能污染细胞培养物的微生物不可渗透的气体交换膜，用于将细胞培养所需的气体供给培养室并排出细胞中产生的气体 培养 细胞培养室可以有利地被配置为支持透析膜和气体交换膜两者的独立模块。 营养供应室可以被配置为具有开口端的杯形模块，所述杯状模块通过卡扣元件在透析膜的位置处可释放地与细胞培养室模块互连。
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公开(公告)号：US5449617A
公开(公告)日：1995-09-12
申请号：US115099
申请日：1993-09-02
IPC分类号： C12M1/02 , C12M1/04 , C12M1/10 , C12M1/12 , C12M1/24 , C12M3/00 , C12M3/04 , C12M3/06 , C12M1/06
CPC分类号： C12M29/04 , C12M23/24 , C12M23/34 , C12M27/02 , Y10S435/818
摘要： Culture vessels for cell cultures having at least one cell culture chamber containing a cell culture mixture that is separated by a dialysis membrane from a nutrient medium in a nutrient supply chamber. Nutrients are transported through the dialysis membrane into the cell culture chamber and metabolic products are transported out of the cell culture chamber into the nutrient supply chamber. To provide a culture vessel for generating cell cultures with a high cell density that is economical to manufacture and easy to handle and to reduce the danger of infections, a gas exchange membrane, that at least partly delimits the cell culture chamber and that is impermeable to liquids and to microorganisms that could contaminate the cell cultures is provided for feeding the gases required for cell culturing to the culture chamber and for discharging the gases generated during cell culturing.
摘要翻译： 用于细胞培养物的培养容器具有至少一个细胞培养室，该细胞培养室含有通过透析膜从营养供应室中的营养培养基分离的细胞培养物混合物。 营养物质通过透析膜转运到细胞培养室中，代谢产物从细胞培养室输送到营养供应室中。 提供用于产生具有高细胞密度的细胞培养物的培养容器，其制造经济且易于处理并降低感染的危险，气体交换膜至少部分地限定细胞培养室，并且是不可渗透的 液体和可能污染细胞培养物的微生物被提供用于将细胞培养所需的气体送入培养室并排出细胞培养过程中产生的气体。
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公开(公告)号：USD370263S
公开(公告)日：1996-05-28
申请号：US29637
申请日：1994-10-12
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5.发明授权公开(公告)号：US06406689B1
公开(公告)日：2002-06-18
申请号：US09261816
申请日：1999-03-03
IPC分类号： A01N6300
CPC分类号： A61K39/0011 , A61K2039/5152 , A61K2039/55505 , A61K2039/55522 , Y10S514/963 , Y10S514/965
摘要： Compositions and methods are provided which can be utilized in active immunization as a prophylactic treatment or a therapeutic treatment for tumors. The compositions are employed as injectable tumor vaccines or as preparations for intratumoral administration and are capable of stimulating immune responses to specific tumor antigens. The tumor vaccines are composed of an antigenic cellular material including a plurality of inactivated tumor cells or tumor cell portions, a depot material, and an immunostimulant adsorbed to the depot material. The depot material with absorbed immunostimulant is mixed with the tumor cells or tumor cell portions to form the vaccine compositions. The preparations for intratumoral administration include the depot material adsorbed immunostimulant without the antigenic cellular material. The immunostimulant adsorbed to the depot material permits release of biologically active quantities of the immunostimulant over a period of time rather than all at once.
摘要翻译： 提供可用于主动免疫的组合物和方法作为肿瘤的预防治疗或治疗性治疗。 该组合物用作可注射肿瘤疫苗或用于肿瘤内给药的制剂，并且能够刺激对特定肿瘤抗原的免疫应答。 肿瘤疫苗由抗原性细胞材料组成，包括多个灭活的肿瘤细胞或肿瘤细胞部分，贮库材料和吸附于贮库材料的免疫刺激剂。 将具有吸收的免疫刺激剂的储库材料与肿瘤细胞或肿瘤细胞部分混合以形成疫苗组合物。 肿瘤内给药的制剂包括没有抗原性细胞材料的药物吸收的免疫刺激剂。 吸附到贮库材料的免疫刺激剂允许在一段时间内而不是同时全部释放生物活性量的免疫刺激剂。
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