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公开(公告)号：US5686301A
公开(公告)日：1997-11-11
申请号：US300628
申请日：1994-09-02
摘要： A cell culture vessel which permits gentle but vigorous intermixing and suspending of the cell culture during rotation. The culture vessel features a cell mixing and suspending device combined with a gas exchange membrane such that ample gas exchange occurs without the need to feed oxygen to the cells. The cell mixing and suspending device comprises a baffle system having at least one, and preferably more than one, mixing elements integral with the gas exchange membrane. Movement of the culture vessel, such as for example by rolling, shaking or swirling, moves the mixing elements such that the cells which are to be cultivated are gently intermixed and stably suspended within the cell culture chamber. Pressure variations within the cell culture vessel are accommodated by pressure equalizing device. The pressure equalizing device preferably comprises pressure sheathing made of gas permeable material positioned to form at least a portion of the outer wall of the supply chamber. The pressure sheathing compresses to accommodate increased pressure within the supply chamber. Moreover, the gas permeable material permits additional gas exchange to occur through the supply chamber. Mixing of the nutrient medium is accomplished during movement of the cell culture vessel by way of the extending pressure finger.
摘要翻译： 一种细胞培养容器，其允许在旋转期间温和但有力地混合和悬浮细胞培养物。 培养容器具有与气体交换膜结合的细胞混合和悬浮装置，使得充分的气体交换发生而不需要向细胞供氧。 电池混合和悬浮装置包括具有与气体交换膜成一体的至少一个，优选多于一个的混合元件的挡板系统。 培养容器的移动，例如通过滚动，摇动或旋转，移动混合元件，使待培养的细胞温和地混合并稳定地悬浮在细胞培养室内。 细胞培养容器内的压力变化由压力均衡装置容纳。 压力平衡装置优选地包括由气体可渗透材料制成的压力护套，其被定位成形成供应室的外壁的至少一部分。 压力护套压缩以适应供应室内的增加的压力。 此外，透气材料允许通过供应室发生额外的气体交换。 营养培养基的混合在细胞培养容器通过延伸压力指的运动期间完成。
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公开(公告)号：US5725123A
公开(公告)日：1998-03-10
申请号：US805653
申请日：1997-02-27
申请人： Hans Otto-Nagels
发明人： Hans Otto-Nagels
CPC分类号： B65D50/045 , C12M23/10 , C12M23/38
摘要： The present invention concerns a system for closing cylindrical containers, such as Petri dishes, for example, with a cover cap whose cover apron presses against the outer surface of the lateral wall of the container. In particular, the present invention provides a cylindrical container and cover system having a simple and convenient, yet secure, closing and opening mechanism which consists of as few individual parts as possible. The cover cap of the present invention has individual elevations on the inner surface of the cover apron. The elevations press against the outer surface of the container walls to hold it in a non-positive manner. The elevations are made as knobs or elongated struts, and are arranged on opposite circular sections of the cover apron in such a way that at least two opposite sections of at least 130.degree. of the periphery of the cover apron have no elevations. In accord with the invention, a simple mechanism for removing the cover cap from the container is also provided. Gripping surfaces are provided on the cover apron at positions approximately 90.degree. to the elevations which permit the cover cap to be loosened from the container and lifted off with a simple and convenient maneuver.
摘要翻译： 本发明涉及一种用于封闭圆柱形容器的系统，例如培养皿，例如具有盖帽，其盖板压靠在容器的侧壁的外表面上。 特别地，本发明提供了一种圆柱形容器和盖系统，其具有简单且方便，牢固的关闭和打开机构，其由尽可能少的单个部件组成。 本发明的盖帽在盖板的内表面上具有单独的高度。 高度压靠容器壁的外表面以使其以非正的方式保持。 凸起形成为旋钮或细长支柱，并且布置在盖皮圈的相对的圆形部分上，使得盖皮圈周边的至少130°的至少两个相对部分没有高度。 根据本发明，还提供了用于从容器移除盖帽的简单机构。 夹板表面设置在盖板上，与允许盖帽从容器松开的高度大约90度的位置处，并且以简单和方便的操纵提起。
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公开(公告)号：US5576211A
公开(公告)日：1996-11-19
申请号：US423575
申请日：1995-04-18
IPC分类号： C12M1/02 , C12M1/04 , C12M1/10 , C12M1/12 , C12M1/24 , C12M3/00 , C12M3/04 , C12M3/06 , C12M1/00
CPC分类号： C12M29/04 , C12M23/24 , C12M23/34 , C12M27/02 , Y10S435/818
摘要： Culture vessels for cell cultures having at least one cell culture chamber containing a cell culture mixture that is separated by a dialysis membrane from a nutrient medium in a nutrient supply chamber. Nutrients are transported through the dialysis membrane into the cell culture chamber and metabolic products are transported out of the cell culture chamber into the nutrient supply chamber. A gas exchange membrane that at least partly delimits the cell culture chamber and that is impermeable to liquids and to microorganisms that could contaminate the cell cultures is provided for feeding the gases required for cell culturing to the culture chamber and for discharging the gases generated during cell culturing. The cell culture chamber can advantageously be configured as a stand-alone module supporting both the dialysis membrane and the gas exchange membrane. The nutrient supply chamber can be configured as a cup-shaped module having an open end releasably interconnectable with the cell culture chamber module at the location of the dialysis membrane, via snap elements.
摘要翻译： 用于细胞培养物的培养容器具有至少一个细胞培养室，该细胞培养室含有通过透析膜从营养供应室中的营养培养基分离的细胞培养物混合物。 营养物质通过透析膜转运到细胞培养室中，代谢产物从细胞培养室输送到营养供应室中。 提供至少部分地限定细胞培养室并且对液体和可能污染细胞培养物的微生物不可渗透的气体交换膜，用于将细胞培养所需的气体供给培养室并排出细胞中产生的气体 培养 细胞培养室可以有利地被配置为支持透析膜和气体交换膜两者的独立模块。 营养供应室可以被配置为具有开口端的杯形模块，所述杯状模块通过卡扣元件在透析膜的位置处可释放地与细胞培养室模块互连。
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公开(公告)号：US5763275A
公开(公告)日：1998-06-09
申请号：US676311
申请日：1996-09-09
摘要： The method relates to the co-culturing of two cell cultures in two cell culture chambers such that the cellular products produced by the co-cultured cells are exchanged between the two cell culture chambers. The apparatus for culturing the cells contains two cell culture chambers separated by a microfiltration membrane which is permeable to medium, gases, and cellular products, and is impermeable to cells. A culture medium supply chamber is adjacent to and separated from the first cell culture chamber by a dialysis membrane which is permeable to medium and gases. The exterior portion of the second cell culture contains a gas-exchange membrane which allows gas exchange between the apparatus and the environment. The apparatus can be in a modular form where the first and second cell culture chambers form one module which is detachable from the culture medium supply chamber which forms the second module. The method and apparatus allows the exchange of media, gases, and cellular products between the two cell cultures while preventing direct contact of the two cell cultures.
摘要翻译： PCT No.PCT / EP95 / 04190 Sec。 371日期1996年9月9日 102（e）1996年9月9日PCT PCT 1995年10月25日PCT公布。 WO96 / 16161 PCT出版物 日期1996年5月30日该方法涉及在两个细胞培养室中共培养两种细胞培养物，使得由共培养细胞产生的细胞产物在两个细胞培养室之间交换。 用于培养细胞的装置包含两个细胞培养室，该细胞培养室由可渗透培养基，气体和细胞产物的微滤膜分离，并且对细胞是不可渗透的。 培养基供应室通过可渗透中等和气体的透析膜与第一细胞培养室相邻并分离。 第二细胞培养物的外部部分包含允许装置和环境之间的气体交换的气体交换膜。 该装置可以是模块化形式，其中第一和第二细胞培养室形成一个可从形成第二模块的培养基供应室拆卸的模块。 该方法和装置允许在两种细胞培养物之间交换介质，气体和细胞产物，同时防止两种细胞培养物的直接接触。
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公开(公告)号：US5702945A
公开(公告)日：1997-12-30
申请号：US595351
申请日：1996-02-01
CPC分类号： C12N5/0068 , A61F2/062 , A61L27/60 , C12M23/24 , C12M23/48 , C12M25/02 , C12M29/04 , C12N2533/40
摘要： The purpose of the present invention is to attain optimal gas exchange, nutrient supply and disposal of metabolic waste products without contamination during high-density cell cultivation of adherent cells. The culture vessel of the present invention provides methods and apparatus for the cultivation of cells on a three-dimensional carrier in a closed cell culture chamber, the supply with oxygen taking place across a gas-permeable gas exchange membrane bordering the cell culture chamber and the supply with nutrients taking place across a dialysis membrane bordering the cell culture chamber, across which nutrients are transported and across which metabolic products are removed, without the danger of contaminating the cells during contact with the tools necessary for manually separating the cells being cultivated from the nutrient medium. The cell culture chamber of the present invention features a vessel that is impermeable to fluids on all sides except for the boundary formed by the dialysis membrane. This makes it possible to move the cell culture intensively within a fluid nutrient supply medium. It is neither necessary to open the cell culture chamber during cultivation to introduce new nutrients, nor is it necessary for the design to include components which reach into the cell culture chamber, and form an additional source of contamination. The gas exchange membrane is detachable to allow removal of the cultivated cells in a uniform layer on the carrier, and the carrier is bio-degradable to eliminate the need to remove the cells from the carrier prior to use, such as in the transplantation of skin for example.
摘要翻译： 本发明的目的是在贴壁细胞的高密度细胞培养过程中达到最佳的气体交换，代谢废物的营养供应和处置而不受污染。 本发明的培养容器提供了在闭孔细胞培养室中在三维载体上培养细胞的方法和装置，在与细胞培养室接壤的透气性气体交换膜和 提供与细胞培养室相邻的透析膜上的营养物质，跨越其间运输营养物质并除去代谢产物，而不会在与手工分离培养的细胞所需的工具接触期间污染细胞的危险 营养培养基。 本发明的细胞培养室具有除透析膜形成的边界以外的所有侧面的流体不可渗透的血管。 这使得可以在流体营养供应介质内集中移动细胞培养物。 在培养期间不需要打开细胞培养室以引入新的营养物，也不需要将设计包括进入细胞培养室的组分，并形成另外的污染源。 气体交换膜是可拆卸的，以允许移除载体上均匀层中的培养细胞，并且载体是生物可降解的，以消除在使用前从载体中除去细胞的需要，例如在皮肤移植中 例如。
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公开(公告)号：US5900374A
公开(公告)日：1999-05-04
申请号：US959488
申请日：1997-10-28
申请人： Hans Otto-Nagels
发明人： Hans Otto-Nagels
CPC分类号： C12M33/00
摘要： The invention concerns a cell culture harvesting device consisting of a scraper head with a blade and a guide strip, the scraper head and the guide strip being connected with one another only by magnetic attraction. The magnetic attraction is achieved by the fact that one of the ends of the scraper head and the guide strip which are turned toward one another have a magnet and the other has either a magnet or a material which can be magnetized by the magnet of the respective counterpart. In this way, the scraper head and the guide strip can be moved synchronously and in parallel at a distance from one another. This has the advantage that the scraper head can be placed into the cell culture vessel before a cell culture is started and can be sterilized together with it thereby eliminating the risk of contamination due to a cell culture harvesting device later being placed into the cell culture vessel. Furthermore, the scraper head can have a net-like collection container arranged on it which collects the cells lifted off the growth surface of the cell culture vessel in a manner that avoids damage to the cells.
摘要翻译： 本发明涉及一种细胞培养收获装置，包括具有刀片和导带的刮刀头，刮刀头和导带仅通过磁吸引力相互连接。 磁吸引力是通过以下事实实现的：刮刀头和引导条之间的一个转向彼此具有磁体，另一个具有磁体或能够由相应的磁体磁化的材料 对应。 以这种方式，刮刀头和引导条可以彼此间隔一段距离地同步地并且平行移动。 这样做的优点在于，可以在开始细胞培养之前将刮刀头放入细胞培养容器中，并与其一起灭菌，从而消除随后将细胞培养物收集装置置于细胞培养容器中的污染风险 。 此外，刮刀头可以具有布置在其上的网状收集容器，其以避免细胞损伤的方式收集从细胞培养容器的生长表面起升的细胞。
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7.发明授权Process for collecting and preparing stored blood, apparatus suitable for said process and use of the apparatus 失效用于收集和制备储存的血液的方法，适用于所述方法和装置使用的装置
公开(公告)号：US5888408A
公开(公告)日：1999-03-30
申请号：US676312
申请日：1996-09-09
申请人： Hans-Otto Nagels
发明人： Hans-Otto Nagels
CPC分类号： A61J1/10 , A61J1/12 , A61J1/16 , Y10T436/25375
摘要： The invention is directed to methods and apparatus for collecting and preparing stored blood that minimizes handling and manipulation of blood bags to thereby reduce the contamination risk. In particular, the invention is directed to methods and apparatus wherein at least one sterilely packed blood bag is provided in a packaging apparatus that supports the blood bag during filling and also serves as the transport container for the blood bag. The packaging apparatus comprises a sterile package for at least one blood bag and has a completely enclosed interior space to hold the blood bag and a closure element used to open up the interior space. The interior space has a volume of at least 500 ml or can be expanded to at least 500 ml by applying an internal pressure directed outwards from the interior space and the interior space can be closed by the closure element. Preferably, the packaging apparatus also serves as a support during centrifugation of the collected blood.
摘要翻译： PCT No.PCT / EP95 / 04675第 371日期1996年9月9日 102（e）1996年9月9日PCT 1995年11月28日PCT PCT。 公开号WO96 / 17577 日期1996年6月13日本发明涉及收集和制备储存血液的方法和装置，其使血袋的处理和操作最小化，从而降低污染风险。 特别地，本发明涉及一种方法和装置，其中在包装装置中提供至少一个无菌包装的血袋，所述包装装置在填充期间支撑血袋，并且还用作血袋的运输容器。 包装设备包括用于至少一个血袋的无菌包装，并且具有完全封闭的内部空间以保持血袋和用于打开内部空间的封闭元件。 内部空间具有至少500ml的体积，或者可以通过施加从内部空间向外指向的内部压力而膨胀至至少500ml，并且内部空间可以由封闭元件封闭。 优选地，包装装置还在收集的血液离心期间用作支持物。
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公开(公告)号：USD370263S
公开(公告)日：1996-05-28
申请号：US29637
申请日：1994-10-12
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公开(公告)号：US5449617A
公开(公告)日：1995-09-12
申请号：US115099
申请日：1993-09-02
IPC分类号： C12M1/02 , C12M1/04 , C12M1/10 , C12M1/12 , C12M1/24 , C12M3/00 , C12M3/04 , C12M3/06 , C12M1/06
CPC分类号： C12M29/04 , C12M23/24 , C12M23/34 , C12M27/02 , Y10S435/818
摘要： Culture vessels for cell cultures having at least one cell culture chamber containing a cell culture mixture that is separated by a dialysis membrane from a nutrient medium in a nutrient supply chamber. Nutrients are transported through the dialysis membrane into the cell culture chamber and metabolic products are transported out of the cell culture chamber into the nutrient supply chamber. To provide a culture vessel for generating cell cultures with a high cell density that is economical to manufacture and easy to handle and to reduce the danger of infections, a gas exchange membrane, that at least partly delimits the cell culture chamber and that is impermeable to liquids and to microorganisms that could contaminate the cell cultures is provided for feeding the gases required for cell culturing to the culture chamber and for discharging the gases generated during cell culturing.
摘要翻译： 用于细胞培养物的培养容器具有至少一个细胞培养室，该细胞培养室含有通过透析膜从营养供应室中的营养培养基分离的细胞培养物混合物。 营养物质通过透析膜转运到细胞培养室中，代谢产物从细胞培养室输送到营养供应室中。 提供用于产生具有高细胞密度的细胞培养物的培养容器，其制造经济且易于处理并降低感染的危险，气体交换膜至少部分地限定细胞培养室，并且是不可渗透的 液体和可能污染细胞培养物的微生物被提供用于将细胞培养所需的气体送入培养室并排出细胞培养过程中产生的气体。
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