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公开(公告)号：US20140072988A1
公开(公告)日：2014-03-13
申请号：US14110035
申请日：2012-04-04
IPC分类号： G01N33/82
CPC分类号： G01N33/82
摘要： Method and test kit for quantitative determination of Vitamin D metabolites in blood, wherein a predetermined amount of blood is pre-analytically immobilised on a solid sorption material. Thereby, hemolysis of the blood has no effect on the analysis of vitamin D metabolites. For quantitative analysis the dried blood spot on the sorption material is dissolved with an aqueous solvent buffer containing detergent, pH 7.0 to 10.0, and the vitamin D metabolites are eluted with a protic organic solution having a permittivity of less than 35. The eluate is analysed for vitamin D metabolites using conventional methods.
摘要翻译： 用于定量测定血液中维生素D代谢物的方法和测试试剂盒，其中将预定量的血液预分析固定在固体吸附材料上。 因此，血液溶血对维生素D代谢物的分析没有影响。 为了进行定量分析，将吸附材料上的干血斑用含有洗涤剂的水溶剂缓冲液（pH 7.0至10.0）溶解，并且维生素D代谢物用介电常数小于35的质子有机溶液洗脱。分析洗脱液 用维生素D代谢物使用常规方法。
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公开(公告)号：US20100068725A1
公开(公告)日：2010-03-18
申请号：US12517754
申请日：2008-01-30
CPC分类号： G01N33/82
摘要： A method for quantitating vitamin D metabolites directly in blood plasma or serum, without the need for prior purification of the vitamin D metabolites, comprising a digestion of the serum proteins with a serine protease such as proteinase K and sequence of steps for inhibiting the proteinase K activity in the competitive binding analysis. The advantages of this method are its high accuracy over the whole range of physiologically relevant values and that it can be easily adapted for a fully automated analysis of serum and plasma samples.
摘要翻译： 一种在血浆或血清中直接定量维生素D代谢物的方法，不需要先前纯化维生素D代谢物，包括用丝氨酸蛋白酶如蛋白酶K消化血清蛋白和抑制蛋白酶K的步骤顺序 竞争性结合分析中的活动。 该方法的优点是在生理相关值的整个范围内具有高精度，并且可以容易地适应于血清和血浆样品的全自动分析。
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公开(公告)号：US07964363B2
公开(公告)日：2011-06-21
申请号：US12517754
申请日：2008-01-30
IPC分类号： G01N33/53
CPC分类号： G01N33/82
摘要： A method for quantitating vitamin D metabolites directly in blood plasma or serum, without the need for prior purification of the vitamin D metabolites, comprising a digestion of the serum proteins with a serine protease such as proteinase K and sequence of steps for inhibiting the proteinase K activity in the competitive binding analysis. The advantages of this method are its high accuracy over the whole range of physiologically relevant values and that it can be easily adapted for a fully automated analysis of serum and plasma samples.
摘要翻译： 一种在血浆或血清中直接定量维生素D代谢物的方法，不需要先前纯化维生素D代谢物，包括用丝氨酸蛋白酶如蛋白酶K消化血清蛋白和抑制蛋白酶K的步骤顺序 竞争性结合分析中的活动。 该方法的优点是在生理相关值的整个范围内具有高精度，并且可以容易地适应于血清和血浆样品的全自动分析。
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公开(公告)号：US09140711B2
公开(公告)日：2015-09-22
申请号：US14110035
申请日：2012-04-04
IPC分类号： G01N33/82
CPC分类号： G01N33/82
摘要： Method and test kit for quantitative determination of Vitamin D metabolites in blood, wherein a predetermined amount of blood is pre-analytically immobilized on a solid sorption material. Thereby, hemolysis of the blood has no effect on the analysis of vitamin D metabolites. For quantitative analysis the dried blood spot on the sorption material is dissolved with an aqueous solvent buffer containing detergent, pH 7.0 to 10.0, and the vitamin D metabolites are eluted with a protic organic solution having a permittivity of less than 35. The eluate is analyzed for vitamin D metabolites using conventional methods.
摘要翻译： 用于定量测定血液中维生素D代谢物的方法和测试试剂盒，其中将预定量的血液预分析固定在固体吸附材料上。 因此，血液溶血对维生素D代谢物的分析没有影响。 为了进行定量分析，将吸附材料上的干血斑用含有洗涤剂的水溶剂缓冲液（pH 7.0至10.0）溶解，并且维生素D代谢物用介电常数小于35的质子有机溶液洗脱。分析洗脱液 用维生素D代谢物使用常规方法。
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