公开(公告)号：US07303906B2

公开(公告)日：2007-12-04

申请号：US10655914

申请日：2003-09-05

申请人： Frederick R. Blattner ,  Gyorgy Posfai ,  Christopher D. Herring ,  Guy Plunkett, III ,  Jeremy Glasner ,  Trevor Twose

发明人： Frederick R. Blattner ,  Gyorgy Posfai ,  Christopher D. Herring ,  Guy Plunkett, III ,  Jeremy Glasner ,  Trevor Twose

IPC分类号： C12N1/20 ,  C12N1/22

CPC分类号： C12N1/08

摘要： The present invention discloses that a bacterium having a genome that is genetically engineered to be at least 10% smaller than the genome of its native parent strain has better transformation competence. Specific E. coli strains, having significantly reduced genome sizes, are disclosed which are highly transformation competent. A medium and methodology is taught which enables transformation efficiencies to be increased further.

摘要翻译： 本发明公开了一种具有遗传工程化至少比其天然亲本菌株基因组小10％的基因组的细菌具有较好的转化能力。 公开了具有显着降低的基因组大小的特异性大肠杆菌菌株，其具有高度转化能力。 介绍了一种可以进一步提高转换效率的介质和方法。

公开(公告)号：US20050032225A1

公开(公告)日：2005-02-10

申请号：US10655914

申请日：2003-09-05

申请人： Frederick Blattner ,  Gyorgy Posfai ,  Christopher Herring ,  Guy Plunkett ,  Jeremy Glasner ,  Trevor Twose

发明人： Frederick Blattner ,  Gyorgy Posfai ,  Christopher Herring ,  Guy Plunkett ,  Jeremy Glasner ,  Trevor Twose

IPC分类号： C12N1/08 ,  C12N1/21 ,  C12N15/74

CPC分类号： C12N1/08

摘要： The present invention discloses that a bacterium having a genome that is genetically engineered to be at least 10% smaller than the genome of its native parent strain has better transformation competence. Specific E. coli strains, having significantly reduced genome sizes, are disclosed which are highly transformation competent. A medium and methodology is taught which enables transformation efficiencies to be increased further.

摘要翻译： 本发明公开了一种具有遗传工程化至少比其天然母本基因组的基因组小10％的基因组的细菌具有更好的转化能力。 公开了具有显着降低的基因组大小的特异性大肠杆菌菌株，其具有高度转化能力。 介绍了一种可以进一步提高转换效率的介质和方法。