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公开(公告)号：US20050032225A1
公开(公告)日：2005-02-10
申请号：US10655914
申请日：2003-09-05
申请人： Frederick Blattner , Gyorgy Posfai , Christopher Herring , Guy Plunkett , Jeremy Glasner , Trevor Twose
发明人： Frederick Blattner , Gyorgy Posfai , Christopher Herring , Guy Plunkett , Jeremy Glasner , Trevor Twose
CPC分类号： C12N1/08
摘要： The present invention discloses that a bacterium having a genome that is genetically engineered to be at least 10% smaller than the genome of its native parent strain has better transformation competence. Specific E. coli strains, having significantly reduced genome sizes, are disclosed which are highly transformation competent. A medium and methodology is taught which enables transformation efficiencies to be increased further.
摘要翻译： 本发明公开了一种具有遗传工程化至少比其天然母本基因组的基因组小10％的基因组的细菌具有更好的转化能力。 公开了具有显着降低的基因组大小的特异性大肠杆菌菌株，其具有高度转化能力。 介绍了一种可以进一步提高转换效率的介质和方法。
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公开(公告)号：US20060270043A1
公开(公告)日：2006-11-30
申请号：US10896739
申请日：2004-07-22
CPC分类号： C12N15/102 , C12N15/70
摘要： The present invention provides a bacterium having a genome that is genetically engineered to be at least 2 to about 20% smaller than the genome of its native parent strain. A bacterium with a smaller genome can produce a commercial product more efficiently. The present invention also provides methods for deleting genes and other DNA sequences from a bacterial genome. The methods provide precise deletions and seldom introduces mutations to the genomic DNA sequences around the deletion sites. Thus, the methods can be used to generate a series of deletions in a bacterium without increasing the possibility of undesired homologous recombination within the genome. In addition, some of the methods provided by the present invention can also be used for replacing a region of a bacterial genome with a desired DNA sequence.
摘要翻译： 本发明提供了具有基因组的细菌，其基因组被改造为比其天然亲本菌株的基因组小至少2至约20％。 具有较小基因组的细菌可以更有效地产生商业产品。 本发明还提供从细菌基因组中删除基因和其他DNA序列的方法。 该方法提供精确的缺失，很少引入缺失位点周围基因组DNA序列的突变。 因此，该方法可用于在细菌中产生一系列缺失，而不增加基因组内不希望的同源重组的可能性。 此外，本发明提供的一些方法也可用于用期望的DNA序列替代细菌基因组的区域。
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公开(公告)号：US20070031969A1
公开(公告)日：2007-02-08
申请号：US11173900
申请日：2005-07-01
CPC分类号： C12N15/102 , C12N1/08 , C12R1/19
摘要： The present invention provides a bacterium having a genome that is genetically engineered to be at least 2 to 14% smaller than the genome of its native parent strain. A bacterium with a smaller genome can produce a commercial product more efficiently. The present invention also provides methods for deleting genes and other DNA sequences from a bacterial genome. The methods provide precise deletions and seldom introduces mutations to the genomic DNA sequences around the deletion sites. Thus, the methods can be used to generate a series of deletions in a bacterium without increasing the possibility of undesired homologous recombination within the genome. In addition, some of the methods provided by the present invention can also be used for replacing a region of a bacterial genome with a desired DNA sequence.
摘要翻译： 本发明提供了一种具有遗传工程化至少比其天然亲本菌株的基因组小至少2至14％的基因组的细菌。 具有较小基因组的细菌可以更有效地产生商业产品。 本发明还提供从细菌基因组中删除基因和其他DNA序列的方法。 该方法提供精确的缺失，很少引入缺失位点周围基因组DNA序列的突变。 因此，该方法可用于在细菌中产生一系列缺失，而不增加基因组内不希望的同源重组的可能性。 此外，本发明提供的一些方法也可用于用期望的DNA序列替代细菌基因组的区域。
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公开(公告)号：US20070054358A1
公开(公告)日：2007-03-08
申请号：US11400711
申请日：2006-04-07
申请人： Frederick Blattner , John Campbell , David Frisch , Guy Plunkett , Gyorgy Posfai
发明人： Frederick Blattner , John Campbell , David Frisch , Guy Plunkett , Gyorgy Posfai
摘要： A bacteria lacking genomic and non-genomic IS elements is provided. The bacteria may be more stable and useful for the production of amino acids, polypeptides, nucleic acids and other products.
摘要翻译： 提供了缺乏基因组和非基因组IS元件的细菌。 细菌可能更稳定并且可用于生产氨基酸，多肽，核酸和其它产物。
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公开(公告)号：US20100304445A1
公开(公告)日：2010-12-02
申请号：US12664899
申请日：2008-06-16
CPC分类号： C07H1/00 , C07H5/10 , C12Q1/686 , C12Q1/6869 , C12Q2565/537 , C12Q2565/515 , C12Q2535/125
摘要： The present invention relates to cloning target nucleic acids using phage packaging mechanisms. Packaging initiation sites may be introduced into the target DNA. Components of a phage packaging system may be combined with the target DNA to package the DNA into phage capsids. The packaged DNA may be used to create a library of target nucleic acids, or it may be sequenced.
摘要翻译： 本发明涉及使用噬菌体包装机制克隆靶核酸。 可以将包装起始位点引入目标DNA。 噬菌体包装系统的组分可以与目标DNA组合以将DNA包装到噬菌体衣壳中。 包装的DNA可用于产生靶核酸文库，或者可以测序。
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6.发明申请COMPOSITIONS COMPRISING REDUCED GENOME BACTERIA FOR USE IN TREATMENT OF SEPSIS 审中-公开包含减少基因细菌的组合物用于治疗SEPSIS公开(公告)号：US20120093865A2
公开(公告)日：2012-04-19
申请号：US13148037
申请日：2010-02-10
IPC分类号： A61K39/02 , A61P31/04 , A61P37/04 , A61K39/108 , C12N1/20
CPC分类号： A61K35/74 , Y02A50/473
摘要： Methods for prophylaxis or treatment of sepsis in a subject are provided comprising administering to the subject a therapeutically effective dose of multiple deletion strain bacteria.
摘要翻译： 提供了用于预防或治疗受试者败血症的方法，包括向受试者施用治疗有效剂量的多种缺失菌株细菌。
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公开(公告)号：US20110053274A1
公开(公告)日：2011-03-03
申请号：US12745443
申请日：2008-11-26
CPC分类号： C12N15/72
摘要： Provided herein is a nucleic acid comprising a mutant lac operator operably linked to a gene of interest, a host cell comprising the nucleic acid, and a method of using the host cell to express the gene of interest. Also provided is a recA-mediated cloning method.
摘要翻译： 本文提供的是包含可操作地连接到目标基因的突变型lac操纵子的核酸，包含该核酸的宿主细胞，以及使用该宿主细胞表达感兴趣的基因的方法。 还提供了recA介导的克隆方法。
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公开(公告)号：US20060199257A1
公开(公告)日：2006-09-07
申请号：US11275094
申请日：2005-12-09
申请人： Frederick Blattner
发明人： Frederick Blattner
IPC分类号： C12N1/21
CPC分类号： C12N15/70
摘要： The present invention provides a bacterium having a genome that is genetically engineered to be smaller than the genome of its native parent strain. A bacterium with a smaller genome can produce a commercial product more efficiently. The present invention also provides methods for deleting genes and other DNA sequences from a bacterial genome. The methods provide precise deletions and seldom introduces mutations to the genomic DNA sequences around the deletion sites. Thus, the methods can be used to generate a series of deletions in a bacterium without increasing the possibility of undesired homologous recombination within the genome. In addition, some of the methods provided by the present invention can also be used for replacing a region of a bacterial genome with a desired DNA sequence.
摘要翻译： 本发明提供了一种具有遗传工程化以小于其天然亲本菌株的基因组的基因组的细菌。 具有较小基因组的细菌可以更有效地产生商业产品。 本发明还提供从细菌基因组中删除基因和其他DNA序列的方法。 该方法提供精确的缺失，很少引入缺失位点周围基因组DNA序列的突变。 因此，该方法可用于在细菌中产生一系列缺失，而不增加基因组内不希望的同源重组的可能性。 此外，本发明提供的一些方法也可用于用期望的DNA序列替代细菌基因组的区域。
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公开(公告)号：US20070178490A1
公开(公告)日：2007-08-02
申请号：US11551970
申请日：2006-10-23
申请人： Frederick Blattner
发明人： Frederick Blattner
CPC分类号： C12N9/22
摘要： An endonuclease which recognizes a specific nucleotide sequence, a gene coding for the endonuclease, a recombinant vector comprising the gene, a host cell comprising the vector, a process for producing the endonuclease, and methods of using the endonuclease are described.
摘要翻译： 描述识别特定核苷酸序列的核酸内切酶，编码内切核酸酶的基因，包含该基因的重组载体，包含载体的宿主细胞，产生内切核酸酶的方法和使用内切核酸酶的方法。
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