公开(公告)号：US20150031128A1

公开(公告)日：2015-01-29

申请号：US14383529

申请日：2013-03-08

Applicant: Friesland Brands B.V.

Inventor： Abhishek Gupta ,  Mireille Maria Gadellaa ,  Dominick Yves Willy Maes

IPC: C12N5/00

CPC classification number: C12N5/0018 ,  C12N5/0043 ,  C12N2500/30 ,  C12N2500/32 ,  C12N2500/33 ,  C12N2500/34 ,  C12N2500/40 ,  C12N2500/76 ,  C12N2500/92 ,  C12N2501/40 ,  C12N2501/999

Abstract: The invention pertains to the use of (i) one or more C2-C6 alpha-hydroxy acids, salts of these acids, esters of these acids and combinations thereof; and (ii) one or more compounds selected from—the group of amino acid derivatives consisting of γ-glutamyl amino acids, pyroglutamyl amino acids, glutamate-containing or proline-containing dipeptides, oxo-aminoacids, homo-amino acids, N-acetyl amino-acids and glycyl-glycine; -phenolic acid derivatives; -linear C2-C6 or cyclic C6 sugar alcohols; -pyridinic acid derivatives; and -nucleobases and/or nucleotides chosen from uracil, adenine, adenosine 3′-monophosphate (3′-AMP) and adenosine 5′-monophosphate (AMP), as a growth-and production promoting ingredient, in culture media for culturing eukaryotic cells. The invention further pertains to culture media containing these components at levels of at least 0.001 mg/l.

Abstract translation: 本发明涉及（i）一种或多种C 2 -C 6α-羟基酸，这些酸的盐，这些酸的酯及其组合的用途; 和（ii）一种或多种选自由γ-谷氨酰氨基酸，焦谷氨酰氨基酸，含谷氨酸或含脯氨酸的二肽组成的氨基酸衍生物，氧代 - 氨基酸，高氨基酸，N-乙酰基 氨基酸和甘氨酰 - 甘氨酸; - 酚酸衍生物; 线性C2-C6或环状C6糖醇; - 吡啶酸衍生物; 和选自尿嘧啶，腺嘌呤，腺苷3'-单磷酸（3-AMP）和腺苷5'-单磷酸（AMP）的核碱基和/或核苷酸作为培养和生产促进成分，用于培养真核细胞 。 本发明还涉及含有至少0.001mg / l水平的这些组分的培养基。

公开(公告)号：US20150017717A1

公开(公告)日：2015-01-15

申请号：US14383534

申请日：2013-03-08

Applicant: Friesland Brands B.V.

Inventor： Abhishek Gupta ,  Mireille Maria Gadellaa ,  Dominick Yves Willy Maes

IPC: C12N5/00

CPC classification number: C12N5/0018 ,  C12N5/0043 ,  C12N2500/30 ,  C12N2500/32 ,  C12N2500/33 ,  C12N2500/34 ,  C12N2500/40 ,  C12N2500/76 ,  C12N2500/92 ,  C12N2501/40 ,  C12N2501/999

Abstract: The invention pertains to the use of C2-C6alpha-hydroxy acids selected from L-3-phenyl lactic acid, mucic (galactaric) acid, gluconic acid, glucaric acid, glyceric acid, 2-hydroxy butyric acid, alpha-hydroxyisovaleric acid, alpha-hydroxyisocaproic acid and erythronic acid and combinations thereof, as a growth- and production promoting ingredient, in culture media for culturing eukaryotic cells. The invention further pertains to culture media containing these alpha-hydroxy acid derivatives at levels of at least 0.001 mg/l.

Abstract translation: 本发明涉及使用选自L-3-苯基乳酸，粘酸（乳酸）酸，葡糖酸，葡糖二酸，甘油酸，2-羟基丁酸，α-羟基异戊酸，α-羟基丁酸的C2-C6-羟基酸 - 羟基异己酸和红霉素酸及其组合作为生长促进成分，用于培养真核细胞的培养基中。 本发明还涉及含有至少0.001mg / l水平的这些α-羟基酸衍生物的培养基。