公开(公告)号：US20230365933A1

公开(公告)日：2023-11-16

申请号：US18246710

申请日：2021-09-28

Applicant: SOCIETE DES PRODUITS NESTLE S.A. ,  LUDWIG INSTITUTE FOR CANCER RESEARCH LTD

Inventor： NICOLA VANNINI ,  MUKUL GIROTRA ,  GEORGE COUKOS

IPC: C12N5/0789 ,  A61K35/28

CPC classification number: C12N5/0647 ,  A61K35/28 ,  C12N2500/38 ,  C12N2500/40 ,  C12N2501/999

Abstract: Use of a composition comprising a combination of a NAD+ precursor, preferably Nicotinamide Riboside and Vitamin B12 for increasing stem cell function in a population of haematopoietic stem and/or progenitor cells (HSPCs).

公开(公告)号：US20230167414A1

公开(公告)日：2023-06-01

申请号：US17921606

申请日：2021-04-26

Applicant: Georgetown University

Inventor： Paula R. Pohlmann ,  Seema Agarwal ,  Richard Schlegel

IPC: C12N5/09 ,  G01N33/574

CPC classification number: C12N5/0693 ,  G01N33/574 ,  C12N2501/999 ,  C12N2513/00 ,  C12N2500/99 ,  C12N2500/40 ,  C12N2500/02

Abstract: Methods of generating a population of tumor cells, such as circulating tumor cells (CTCs) isolated from fluid from a subject. The methods involve collecting a fluid sample containing the tumor cells from the subject, and culturing the tumor cells in the fluid sample in a three-dimensional cell culture, wherein the three-dimensional cell culture comprises at least one inhibitor of Rho-kinase to generate the population of CTCs. If the fluid is whole blood or contains blood, the method may also involve subjecting the fluid sample to density gradient separation to separate the tumor cells from the fluid prior to culturing. In addition, methods of identifying a candidate treatment for a subject having a condition marked by the presence of tumor cells, methods of monitoring in a subject the persistence, regression, or progression of a disease or condition marked by the presence of tumor cells, and methods of generating a cell line of tumor cells.

公开(公告)号：US20180325957A1

公开(公告)日：2018-11-15

申请号：US15769873

申请日：2016-10-21

Applicant: Rigshospitalet

Inventor： Jens Kastrup ,  Annette Ekblond ,  Mandana Haack-Sørensen

IPC: A61K35/28 ,  A61P29/00 ,  A61P37/06 ,  A61K38/19 ,  A61K9/00 ,  C12N5/0775

CPC classification number: A61K35/28 ,  A61K9/0019 ,  A61K38/19 ,  A61P3/10 ,  A61P19/02 ,  A61P19/08 ,  A61P29/00 ,  A61P37/06 ,  C12N5/0667 ,  C12N2500/34 ,  C12N2500/38 ,  C12N2500/40 ,  C12N2500/44 ,  C12N2500/60 ,  C12N2500/62 ,  C12N2500/84

Abstract: The present invention relates to adipose-derived stem cells (ASCs) and compositions, as well as methods for preparing and using such ASCs and compositions for therapy.

公开(公告)号：US09499785B2

公开(公告)日：2016-11-22

申请号：US12446801

申请日：2007-10-24

Applicant: Ivan Vassiliev ,  Mark Brenton Nottle

Inventor： Ivan Vassiliev ,  Mark Brenton Nottle

IPC: C12N15/00 ,  C12N5/073 ,  C12N5/0735

CPC classification number: C12N5/0606 ,  C12N5/0604 ,  C12N2500/25 ,  C12N2500/32 ,  C12N2500/40 ,  C12N2500/44 ,  C12N2500/84 ,  C12N2500/90 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/235 ,  C12N2502/13

Abstract: The present invention provides a method of isolating a pluripotent cell from a pre-implantation embryo without isolation of the pluripotent cells from other cells, the method including propagating a whole pre-implantation embryo including one or more pluripotent cells, embedded in a feeder cell layer and cultivated in a medium substantially free of serum, and isolating a pluripotent cell from the one or more pluripotent cells. The present invention also provides pluripotent cells generated by the method and uses thereof.

Abstract translation: 本发明提供了从植入前的胚胎中分离多能细胞而不从其它细胞分离多能细胞的方法，所述方法包括传播包含一个或多个多能细胞的整个植入前胚胎，其嵌入饲养细胞层 并在基本上不含血清的培养基中培养，并从一个或多个多能细胞中分离出多能细胞。 本发明还提供通过其方法和用途产生的多能细胞。

公开(公告)号：US20160289644A1

公开(公告)日：2016-10-06

申请号：US15040783

申请日：2016-02-10

Applicant: Georgetown University

Inventor： Richard Schlegel ,  Xuefeng Liu

IPC: C12N5/071

CPC classification number: C12N5/0683 ,  C12N5/0625 ,  C12N5/0631 ,  C12N5/067 ,  C12N5/0688 ,  C12N2500/05 ,  C12N2500/40 ,  C12N2501/01 ,  C12N2501/11 ,  C12N2501/33 ,  C12N2501/39 ,  C12N2501/727 ,  C12N2502/13 ,  C12N2502/1323 ,  C12N2503/02 ,  C12N2510/04 ,  C12Q1/6883 ,  C12Q2600/106 ,  C12Q2600/158 ,  G01N33/5091

Abstract: The present invention is directed towards methods of culturing non-keratinocyte epithelial cells, with the methods comprising culturing non-keratinocyte epithelial cells in the presence of feeder cells and a calcium-containing medium while inhibiting the activity of Rho kinase (ROCK) in the feeder cell, the non-keratinocyte epithelial cells or both during culturing.

公开(公告)号：US20160237399A1

公开(公告)日：2016-08-18

申请号：US14625559

申请日：2015-02-18

Applicant: Biogen MA Inc.

Inventor： William YANG ,  Yao-Ming Huang ,  Kyle McElearney ,  Lia Tescione ,  James Lambropoulos ,  An Zhang ,  Valerie Tsang ,  Thomas Ryll

IPC: C12N5/00 ,  C12P21/00

CPC classification number: C12P21/005 ,  C12N5/0018 ,  C12N2500/20 ,  C12N2500/30 ,  C12N2500/34 ,  C12N2500/40 ,  C12N2500/90 ,  C12N2501/04 ,  C12N2511/00

Abstract: The present invention pertains to a cell culture medium comprising media supplements that are shown to control recombinant protein glycosylation and/or cell culture in a controlled or modulated (shifted) temperature to control recombinant protein glycosylation and/or cell culture with controlled or modulated seed density to control recombinant protein glycosylation, and methods of using thereof. The present invention further pertains to a method of controlling or manipulating glycosylation of a recombinant protein of interest in a large scale cell culture.

Abstract translation: 本发明涉及包含培养基补充物的细胞培养基，其显示为控制或调节（转移）温度中的重组蛋白糖基化和/或细胞培养以控制具有受控或调节的种子密度的重组蛋白糖基化和/或细胞培养 以控制重组蛋白糖基化，及其使用方法。 本发明还涉及在大规模细胞培养物中控制或操纵感兴趣的重组蛋白质的糖基化的方法。

公开(公告)号：US20150361390A1

公开(公告)日：2015-12-17

申请号：US14764226

申请日：2014-02-03

Applicant: Conradus Ghosal GHO

Inventor： Conradus Ghosal Gho

IPC: C12N5/071 ,  A61K35/12

CPC classification number: C12N5/0602 ,  A61K35/12 ,  C12N5/0628 ,  C12N5/0666 ,  C12N2500/20 ,  C12N2500/24 ,  C12N2500/38 ,  C12N2500/40 ,  C12N2500/44 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/13 ,  C12N2501/14 ,  C12N2501/999 ,  C12N2502/11 ,  C12N2502/115 ,  C12N2506/03 ,  C12N2509/00

Abstract: The present invention is concerned with a composition and in vitro method for generating a desired cell type and/or tissue type from hair follicular stem cells. The composition and in vitro method are particularly suitable for generating an autologous desired cell type and/or tissue type. Furthermore, the composition and method are especially efficient and suitable for use in the context of cosmetic cell and/or tissue transplantation in recipient areas of a subject experiencing cell and/or tissue loss caused by, for example, a wound, scar, burn injury, tissue degeneration, and aging. The composition and in vitro method are also suitable to circumvent complications related to infections and/or immune rejection of a cosmetic cell and/or tissue implant or graft.

Abstract translation: 本发明涉及从毛囊干细胞产生所需细胞类型和/或组织类型的组合物和体外方法。 组合物和体外方法特别适合于产生自体期望的细胞类型和/或组织类型。 此外，组合物和方法特别有效并且适合用于经历由例如伤口，瘢痕，烧伤引起的细胞和/或组织损失的受试者的受体区域中的化妆品细胞和/或组织移植的上下文中 ，组织变性和衰老。 组合物和体外方法也适用于避免与化妆品细胞和/或组织植入物或移植物的感染和/或免疫排斥相关的并发症。

公开(公告)号：US20150299660A1

公开(公告)日：2015-10-22

申请号：US14441083

申请日：2013-11-14

Applicant: International Stem Cell Corporation

Inventor： Trudy Christiansen-Weber

IPC: C12N5/0775

CPC classification number: C12N5/0662 ,  C12N2500/12 ,  C12N2500/20 ,  C12N2500/22 ,  C12N2500/25 ,  C12N2500/32 ,  C12N2500/38 ,  C12N2500/40 ,  C12N2500/46 ,  C12N2501/115 ,  C12N2501/385 ,  C12N2506/1307

Abstract: The present invention is based in part on methods of differentiating fibroblast cells into adipocytes, osteocytes and chondrocytes. Additionally, the present invention provides agents and kits useful for differentiating fibroblast cells in adipocytes, osteocytes and chondrocytes. Further, the present invention provides for enhanced extracellular matrix deposition using complex sugars.

Abstract translation: 本发明部分基于将成纤维细胞分化成脂肪细胞，骨细胞和软骨细胞的方法。 此外，本发明提供了可用于在脂肪细胞，骨细胞和软骨细胞中分化成纤维细胞的试剂和试剂盒。 此外，本发明提供使用复合糖的增强的细胞外基质沉积。

公开(公告)号：US20150044769A1

公开(公告)日：2015-02-12

申请号：US14186848

申请日：2014-02-21

Applicant: Kyokuto Pharmaceutical Industrial Co., Ltd.

Inventor： Tetsuji Sasaki

IPC: C12N5/00 ,  C12N5/071

CPC classification number: C12N5/005 ,  C12N5/0682 ,  C12N2500/34 ,  C12N2500/36 ,  C12N2500/40 ,  C12N2500/46 ,  C12N2500/95 ,  C12N2501/11 ,  C12N2501/33

Abstract: A cell of a cell line adapted to a protein-free and lipid-free medium, which is derived from CHO cells, can be stably used for production of recombinant proteins and can proliferate in a suspended state in a protein-free and lipid-free medium containing no exogenous growth factors. A method for adapting CHO cells by using a protein-free and lipid-free medium and a medium used for the method.

Abstract translation: 适用于无细胞和不含脂质的培养基（由CHO细胞衍生的细胞系）的细胞可以稳定地用于重组蛋白质的生产，并可以悬浮状态以无蛋白质和无脂质的形式增殖 不含外源生长因子的培养基。 通过使用无蛋白质和无脂质的培养基和用于该方法的培养基来适应CHO细胞的方法。

公开(公告)号：US20150017717A1

公开(公告)日：2015-01-15

申请号：US14383534

申请日：2013-03-08

Applicant: Friesland Brands B.V.

Inventor： Abhishek Gupta ,  Mireille Maria Gadellaa ,  Dominick Yves Willy Maes

IPC: C12N5/00

CPC classification number: C12N5/0018 ,  C12N5/0043 ,  C12N2500/30 ,  C12N2500/32 ,  C12N2500/33 ,  C12N2500/34 ,  C12N2500/40 ,  C12N2500/76 ,  C12N2500/92 ,  C12N2501/40 ,  C12N2501/999

Abstract: The invention pertains to the use of C2-C6alpha-hydroxy acids selected from L-3-phenyl lactic acid, mucic (galactaric) acid, gluconic acid, glucaric acid, glyceric acid, 2-hydroxy butyric acid, alpha-hydroxyisovaleric acid, alpha-hydroxyisocaproic acid and erythronic acid and combinations thereof, as a growth- and production promoting ingredient, in culture media for culturing eukaryotic cells. The invention further pertains to culture media containing these alpha-hydroxy acid derivatives at levels of at least 0.001 mg/l.

Abstract translation: 本发明涉及使用选自L-3-苯基乳酸，粘酸（乳酸）酸，葡糖酸，葡糖二酸，甘油酸，2-羟基丁酸，α-羟基异戊酸，α-羟基丁酸的C2-C6-羟基酸 - 羟基异己酸和红霉素酸及其组合作为生长促进成分，用于培养真核细胞的培养基中。 本发明还涉及含有至少0.001mg / l水平的这些α-羟基酸衍生物的培养基。