Probe correction for gene expression level detection
    3.
    发明授权
    Probe correction for gene expression level detection 有权
    基因表达水平检测的探针校正

    公开(公告)号:US07715990B2

    公开(公告)日:2010-05-11

    申请号:US10500587

    申请日:2003-01-17

    IPC分类号: G01N33/483

    摘要: Individual probes on micro-arrays are re-scaled and corrected with a set of probe dependent coefficients derived from genomic-DNA hybridization signals. A dynamic range for gDNA binding is determined by measuring a concentration signal curve. Signals for each probe are measured during multiple hybridizations within a linear range. Concentration insensitive probes are then found for two sets of experiments. Probes are discarded based on a threshold compared to their standard deviation divided by their average in each set. The correction coefficients are used to calculate a corrected intensity for each probe. Probes having high uncertainty (0.5 in one embodiment) are discarded. A weighting factor for each probe is determined along with an uncertainty factor. Finally, a call for each gene is made, such as absent, marginal or present.

    摘要翻译: 使用从基因组DNA杂交信号衍生的一组探针依赖系数对微阵列上的单个探针进行重新定标和校正。 通过测量浓度信号曲线确定gDNA结合的动态范围。 在线性范围内的多次杂交期间测量每个探针的信号。 然后发现两组实验的浓度不敏感探针。 探针根据其标准偏差除以每组中的平均值的阈值进行丢弃。 校正系数用于计算每个探针的校正强度。 具有高不确定度的探针(在一个实施例中为0.5)被丢弃。 确定每个探头的加权系数以及不确定因素。 最后,调用每个基因,如缺席,边缘或存在。