BPMV-based viral constructs useful for VIGS and expression of heterologous proteins in legumes
    3.
    发明授权
    BPMV-based viral constructs useful for VIGS and expression of heterologous proteins in legumes 有权
    基于BPMV的病毒构建体可用于VIGS并在豆类中表达异源蛋白质

    公开(公告)号:US08569579B2

    公开(公告)日:2013-10-29

    申请号:US12927579

    申请日:2010-11-18

    IPC分类号: C12N15/83 C12N15/33 C12N5/10

    CPC分类号: C12N15/8203 C12N15/8218

    摘要: The invention provides Bean pod mottle virus (BPMV) vectors useful for expression of heterologous proteins in plants such as soybean. The BPMV vectors are also useful for virus-induced gene silencing. The vectors of the invention include modifications of BPMV RNA1 sequences so that infection with the vectors produces only moderate symptoms. The vectors also comprise novel RNA2 vectors which specifically provide for non-translated VIGS constructs and further which do not require in frame insertion of heterologous sequences to be expressed.

    摘要翻译: 本发明提供了可用于在植物如大豆中表达异源蛋白质的豆荚斑驳病毒(BPMV)载体。 BPMV载体也可用于病毒诱导的基因沉默。 本发明的载体包括BPMV RNA1序列的修饰,使得与载体的感染仅产生中度症状。 载体还包含特异性提供未翻译的VIGS构建体的新型RNA2载体,并且进一步不需要在框架中插入待表达的异源序列。

    BPMV-based viral constructs useful for VIGS and expression of heterologous proteins in legumes
    4.
    发明申请
    BPMV-based viral constructs useful for VIGS and expression of heterologous proteins in legumes 有权
    基于BPMV的病毒构建体可用于VIGS并在豆类中表达异源蛋白质

    公开(公告)号:US20110173717A1

    公开(公告)日:2011-07-14

    申请号:US12927579

    申请日:2010-11-18

    IPC分类号: C12N15/83 C07H21/02

    CPC分类号: C12N15/8203 C12N15/8218

    摘要: The invention provides Bean pod mottle virus (BPMV) vectors useful for expression of heterologous proteins in plants such as soybean. The BPMV vectors are also useful for virus-induced gene silencing. The vectors of the invention include modifications of BPMV RNA1 sequences so that infection with the vectors produces only moderate symptoms. The vectors also comprise novel RNA2 vectors which specifically provide for non-translated VIGS constructs and further which do not require in frame insertion of heterologous sequences to be expressed.

    摘要翻译: 本发明提供了可用于在植物如大豆中表达异源蛋白质的豆荚斑驳病毒(BPMV)载体。 BPMV载体也可用于病毒诱导的基因沉默。 本发明的载体包括BPMV RNA1序列的修饰,使得与载体的感染仅产生中度症状。 载体还包含特异性提供未翻译的VIGS构建体的新型RNA2载体,并且进一步不需要在框架中插入待表达的异源序列。