公开(公告)号：US08410062B2

公开(公告)日：2013-04-02

申请号：US13523438

申请日：2012-06-14

申请人： Fumihito Sugihara ,  Naoki Inoue ,  Seiko Koizumi ,  Chinfang Liu ,  Hajime Takasaki ,  Hisayuki Kobayashi ,  Hiroshi Mano ,  Sachie Nakatani ,  Masahiro Wada

发明人： Fumihito Sugihara ,  Naoki Inoue ,  Seiko Koizumi ,  Chinfang Liu ,  Hajime Takasaki ,  Hisayuki Kobayashi ,  Hiroshi Mano ,  Sachie Nakatani ,  Masahiro Wada

IPC分类号： A61K38/05

CPC分类号： C07K5/06139 ,  A61K38/05 ,  C07K5/06165

摘要： A problem that the present invention is to solve is to provide: a main body of a peptide molecule which is effective for inhibition of various maladies such as osteoporosis, osteoarthritis and pressure ulcer, particularly, a dipeptide which is easy to absorb into a body in an intestine; a collagen peptide which comprises the dipeptide as an essential dipeptide; and a malady inhibitor which comprises the dipeptide as an essential effective component. As a means of solving such a problem, a collagen peptide according to the present invention is characterized by comprising a dipeptide having a structure of Hyp-Gly as an essential dipeptide. A dipeptide according to the present invention is characterized by having a structure of Hyp-Gly. A malady inhibitor according to the present invention is characterized by comprising a dipeptide having a structure of Hyp-Gly as an essential effective component.

摘要翻译： 本发明要解决的问题是提供：对于抑制诸如骨质疏松症，骨关节炎和压力性溃疡等各种疾病的肽分子的主体，特别是容易吸收到体内的二肽 肠 包含二肽作为必需二肽的胶原肽; 以及包含二肽作为基本有效成分的疾病抑制剂。 作为解决上述问题的方法，本发明的胶原肽的特征在于，含有具有Hyp-Gly结构的二肽作为必需二肽的二肽。 根据本发明的二肽的特征在于具有Hyp-Gly的结构。 根据本发明的疾病抑制剂的特征在于包含具有Hyp-Gly结构作为必需有效成分的二肽。

公开(公告)号：US20110166365A1

公开(公告)日：2011-07-07

申请号：US12443298

申请日：2008-11-14

申请人： Fumihito Sugihara ,  Naoki Inoue ,  Seiko Koizumi ,  Chinfang Liu ,  Hajime Takasaki ,  Hisayuki Kobayashi ,  Hiroshi Mano ,  Sachie Nakatani ,  Masahiro Wada

发明人： Fumihito Sugihara ,  Naoki Inoue ,  Seiko Koizumi ,  Chinfang Liu ,  Hajime Takasaki ,  Hisayuki Kobayashi ,  Hiroshi Mano ,  Sachie Nakatani ,  Masahiro Wada

IPC分类号： C07D207/12

CPC分类号： C07K5/06139 ,  A61K38/05 ,  C07K5/06165

摘要： A problem that the present invention is to solve is to provide: a main body of a peptide molecule which is effective for inhibition of various maladies such as osteoporosis, osteoarthritis and pressure ulcer, particularly, a dipeptide which is easy to absorb into a body in an intestine; a collagen peptide which comprises the dipeptide as an essential dipeptide; and a malady inhibitor which comprises the dipeptide as an essential effective component. As a means of solving such a problem, a collagen peptide according to the present invention is characterized by comprising a dipeptide having a structure of Hyp-Gly as an essential dipeptide. A dipeptide according to the present invention is characterized by having a structure of Hyp-Gly. A malady inhibitor according to the present invention is characterized by comprising a dipeptide having a structure of Hyp-Gly as an essential effective component.

摘要翻译： 本发明要解决的问题是提供：对于抑制诸如骨质疏松症，骨关节炎和压力性溃疡等各种疾病的肽分子的主体，特别是容易吸收到体内的二肽 肠 包含二肽作为必需二肽的胶原肽; 以及包含二肽作为基本有效成分的疾病抑制剂。 作为解决上述问题的方法，本发明的胶原肽的特征在于，含有具有Hyp-Gly结构的二肽作为必需二肽的二肽。 根据本发明的二肽的特征在于具有Hyp-Gly的结构。 根据本发明的疾病抑制剂的特征在于包含具有Hyp-Gly结构作为必需有效成分的二肽。

公开(公告)号：US08227424B2

公开(公告)日：2012-07-24

申请号：US12443298

申请日：2008-11-14

申请人： Fumihito Sugihara ,  Naoki Inoue ,  Seiko Koizumi ,  Chinfang Liu ,  Hajime Takasaki ,  Hisayuki Kobayashi ,  Hiroshi Mano ,  Sachie Nakatani ,  Masahiro Wada

发明人： Fumihito Sugihara ,  Naoki Inoue ,  Seiko Koizumi ,  Chinfang Liu ,  Hajime Takasaki ,  Hisayuki Kobayashi ,  Hiroshi Mano ,  Sachie Nakatani ,  Masahiro Wada

IPC分类号： A61K38/05 ,  A61P10/02

CPC分类号： C07K5/06139 ,  A61K38/05 ,  C07K5/06165

摘要： A problem that the present invention is to solve is to provide: a main body of a peptide molecule which is effective for inhibition of various maladies such as osteoporosis, osteoarthritis and pressure ulcer, particularly, a dipeptide which is easy to absorb into a body in an intestine; a collagen peptide which comprises the dipeptide as an essential dipeptide; and a malady inhibitor which comprises the dipeptide as an essential effective component. As a means of solving such a problem, a collagen peptide according to the present invention is characterized by comprising a dipeptide having a structure of Hyp-Gly as an essential dipeptide. A dipeptide according to the present invention is characterized by having a structure of Hyp-Gly. A malady inhibitor according to the present invention is characterized by comprising a dipeptide having a structure of Hyp-Gly as an essential effective component.

公开(公告)号：US20070188547A1

公开(公告)日：2007-08-16

申请号：US11505247

申请日：2006-08-16

申请人： Hisayuki Kobayashi ,  Shinichi Uehara ,  Masaru Ohnishi

发明人： Hisayuki Kobayashi ,  Shinichi Uehara ,  Masaru Ohnishi

IPC分类号： B41J23/00

CPC分类号： B41J3/407 ,  B41J2/2114 ,  B41J11/0015 ,  B41J11/002

摘要： An ink-jet printer includes an ink-jet head. The ink-jet head has an image printing head and a ground-color-layer printing head. The image printing head is configured to print an image on a medium and has at least one image printing ink discharge port. The ground-color-layer printing head is configured to print a ground-color-layer on the medium and has at least one ground-color-layer printing ink discharge port. The medium and the ink-jet head are configured to relatively move parallel to a first line and along a second direction substantially perpendicular to the first line. A position of the ground-color-layer printing ink discharge port is displaced from a position of the at least one image printing ink discharge port along the first line and the second direction.

摘要翻译： 喷墨打印机包括喷墨头。 喷墨头具有图像打印头和底色层打印头。 图像打印头被配置为在介质上打印图像，并且具有至少一个图像打印墨水排出口。 地色层打印头被配置为在介质上打印地色层，并且具有至少一个地色层印刷油墨排出口。 介质和喷墨头构造成相对于第一线平行移动并沿着基本上垂直于第一线的第二方向相对移动。 地色层印刷油墨排出口的位置沿着第一线和第二方向从至少一个图像印刷油墨排出口的位置移位。

公开(公告)号：US20110316924A1

公开(公告)日：2011-12-29

申请号：US13254858

申请日：2009-03-13

申请人： Hisayuki Kobayashi ,  Takaaki Kambara

发明人： Hisayuki Kobayashi ,  Takaaki Kambara

IPC分类号： B41J29/38

CPC分类号： B41J11/001 ,  B41J11/06

摘要： A printer apparatus (10) is provided with a front and rear drive motor, which rotationally drives a feed roller (21) in a state that the plate-like medium (B) is pinched by the feed roller (21) and a pinch roller (24) which are paired to feed the plate-like medium (B) to the front side and to set to be located on the platen (13), a right and left drive motor which moves the printer head (32) in the right and left direction, and a controller which performs control for feeding the plate-like medium (B) and control for moving the printer head by the right and left drive motor (32). The platen (13) is provided with a feeding auxiliary member (40) which is protruded from a support face for supporting the plate-like medium (B) and is located on the front side with respect to a portion facing the printer head (32).

摘要翻译： 一种打印机装置（10）具有前驱动马达和后驱动马达，该驱动马达在板状介质（B）被进给辊（21）夹持的状态下旋转地驱动进给辊（21），夹送辊 （24），其配对以将板状介质（B）输送到前侧并设置在台板（13）上;右和左驱动马达，其将打印头（32）向右移动 和左方向的控制器，以及执行用于馈送板状介质（B）的控制和由左右驱动电机（32）移动打印头的控制的控制器。 压板（13）设置有从用于支撑板状介质（B）的支撑面突出的进给辅助构件（40），并且位于相对于面向打印头（32）的部分的前侧 ）。

公开(公告)号：US07247481B2

公开(公告)日：2007-07-24

申请号：US10122220

申请日：2002-04-16

申请人： Kazuhiko Minamigawa ,  Hisayuki Kobayashi ,  Toshikazu Takano

发明人： Kazuhiko Minamigawa ,  Hisayuki Kobayashi ,  Toshikazu Takano

IPC分类号： C12N5/06 ,  C12N5/08 ,  C12N11/02

CPC分类号： C12N5/0018 ,  C12N2503/00

摘要： The present invention provide: a novel process for culturing animal cells and a kit for culturing animal cells, in which, even if the number of cells as sampled for biopsy is extremely small, the proliferation can sufficiently be maintained so as to enable to carry out various culture and/or tests, especially anticancer agent sensitivity tests, and the contamination with bacteria can be inhibited without damaging physiological activity of cells, especially sensitivity to anticancer agents. The process for culturing animal cells, according to the present invention, comprises the step of culturing a sample containing animal cells obtained from living body tissue in order to subject the sample to further culture and/or a test, with the process being characterized in that a culture medium is used wherein the culture medium has a proliferating action and physiological activity-retaining action on the animal cells, and further has a killing action and/or multiplication-inhibition action on bacteria.

摘要翻译： 本发明提供：用于培养动物细胞的新方法和用于培养动物细胞的试剂盒，其中即使用于活组织检查取样的细胞数量非常少，可以充分地维持增殖，从而能够进行 可以抑制各种培养和/或试验，特别是抗癌剂敏感性试验，并且可以抑制细菌的污染，而不损害细胞的生理活性，特别是对抗癌剂的敏感性。 根据本发明的培养动物细胞的方法包括培养含有从活体组织获得的动物细胞的样品以使样品进一步培养和/或测试的步骤，其过程的特征在于 使用其中培养基对动物细胞具有增殖作用和生理活性保持作用的培养基，并且还对细菌具有杀伤作用和/或增殖抑制作用。

公开(公告)号：US06201256B1

公开(公告)日：2001-03-13

申请号：US09008760

申请日：1998-01-19

申请人： Yuji Kouchi ,  Hisayuki Kobayashi

发明人： Yuji Kouchi ,  Hisayuki Kobayashi

IPC分类号： G06K900

CPC分类号： B26D5/34 ,  B23Q15/225 ,  B26D5/005 ,  B26F1/3806 ,  G05B2219/42222

摘要： A cutting plotter equipped with a device for reading register marks for seal cutting capable of accurately reading the register marks printed on a seal material. The cutting plotter includes a head provided thereon with a light emitting element for emitting a predetermined amount of light toward a surface of the seal material and a light receiving element for detecting the amount of light reflected from the surface of the seal material. A difference between the amounts of reflected light detected by the light receiving element is calculated by a first calculation unit and then stored in a storage unit. A relative difference between the difference in the amounts of reflected light stored in the storage unit and a difference between the amounts of reflected light calculated by the first calculation unit is calculated by a second calculation unit. Also, a determination unit is arranged so as to determine whether or not the relative difference calculated by the second calculation unit is within a predetermined tolerance, to thereby determine whether or not the register marks are printed on the seal material.

摘要翻译： 一台切割机配有一个用于读取对准标记的装置，用于密封切割，能够准确地读取印在密封材料上的对准标记。 切割机包括设置有用于向密封材料的表面发射预定量的光的发光元件的头部和用于检测从密封材料的表面反射的光量的光接收元件。 由光接收元件检测的反射光量之差由第一计算单元计算，然后存储在存储单元中。 通过第二计算单元计算存储在存储单元中的反射光的量的差与由第一计算单元计算的反射光的差之间的相对差。 此外，确定单元被布置以便确定由第二计算单元计算的相对差是否在预定公差内，从而确定对准标记是否被打印在密封材料上。

公开(公告)号：US08721067B2

公开(公告)日：2014-05-13

申请号：US13254858

申请日：2009-03-13

申请人： Hisayuki Kobayashi ,  Takaaki Kambara

发明人： Hisayuki Kobayashi ,  Takaaki Kambara

IPC分类号： B41J2/01

CPC分类号： B41J11/001 ,  B41J11/06

摘要： A printer apparatus (10) is provided with a front and rear drive motor, which rotationally drives a feed roller (21) in a state that the plate-like medium (B) is pinched by the feed roller (21) and a pinch roller (24) which are paired to feed the plate-like medium (B) to the front side and to set to be located on the platen (13), a right and left drive motor which moves the printer head (32) in the right and left direction, and a controller which performs control for feeding the plate-like medium (B) and control for moving the printer head by the right and left drive motor (32). The platen (13) is provided with a feeding auxiliary member (40) which is protruded from a support face for supporting the plate-like medium (B) and is located on the front side with respect to a portion facing the printer head (32).

摘要翻译： 一种打印机装置（10）具有前驱动马达和后驱动马达，该驱动马达在板状介质（B）被进给辊（21）夹持的状态下旋转地驱动进给辊（21），夹送辊 （24），其配对以将板状介质（B）输送到前侧并设置在台板（13）上;右和左驱动马达，其将打印头（32）向右移动 和左方向的控制器，以及执行用于馈送板状介质（B）的控制和由左右驱动电机（32）移动打印头的控制的控制器。 压板（13）设置有从用于支撑板状介质（B）的支撑面突出的进给辅助构件（40），并且位于相对于面向打印头（32）的部分的前侧 ）。

公开(公告)号：US07798602B2

公开(公告)日：2010-09-21

申请号：US11505247

申请日：2006-08-16

申请人： Hisayuki Kobayashi ,  Shinichi Uehara ,  Masaru Ohnishi

发明人： Hisayuki Kobayashi ,  Shinichi Uehara ,  Masaru Ohnishi

IPC分类号： B41J23/00

CPC分类号： B41J3/407 ,  B41J2/2114 ,  B41J11/0015 ,  B41J11/002

摘要： An ink-jet printer includes an ink-jet head. The ink-jet head has an image printing head and a ground-color-layer printing head. The image printing head is configured to print an image on a medium and has at least one image printing ink discharge port. The ground-color-layer printing head is configured to print a ground-color-layer on the medium and has at least one ground-color-layer printing ink discharge port. The medium and the ink-jet head are configured to relatively move parallel to a first line and along a second direction substantially perpendicular to the first line. A position of the ground-color-layer printing ink discharge port is displaced from a position of the at least one image printing ink discharge port along the first line and the second direction.

摘要翻译： 喷墨打印机包括喷墨头。 喷墨头具有图像打印头和底色层打印头。 图像打印头被配置为在介质上打印图像，并且具有至少一个图像打印墨水排出口。 地色层打印头被配置为在介质上打印地色层，并且具有至少一个地色层印刷油墨排出口。 介质和喷墨头构造成相对于第一线平行移动并沿着基本上垂直于第一线的第二方向相对移动。 地色层印刷油墨排出口的位置沿着第一线和第二方向从至少一个图像印刷油墨排出口的位置移位。

公开(公告)号：US5712161A

公开(公告)日：1998-01-27

申请号：US507418

申请日：1995-08-24

申请人： Masahiro Koezuka ,  Naohito Kondo ,  Hisayuki Kobayashi ,  Hiroshi Saeki ,  Keizo Tanisaka ,  Sachiko Oda

发明人： Masahiro Koezuka ,  Naohito Kondo ,  Hisayuki Kobayashi ,  Hiroshi Saeki ,  Keizo Tanisaka ,  Sachiko Oda

IPC分类号： C12N5/00 ,  C12N5/08 ,  C12N1/02 ,  C12N11/04 ,  C12Q1/02

CPC分类号： C12N5/0012 ,  C12N2533/54

摘要： Animal cells are cultured while embedded in a collagen gel. The gel containing cells is formed by dispersing animal cells in a collagen solution, placing a drop (or drops) of the cell-containing collagen solution on a support surface and allowing the drop to gel to fix on the surface as a globular collagen gel having a convex surface. The cells are cultured by contacting the gel with a culture medium that may be serum-free or contain dextran sulfate. The drop preferably contains about 3 to about 300 microliters of the collagen solution and is about 2 mm or less in height. The cells may be precultured on a support surface having a collagen layer, released from the collagen layer by treatment with collagenase and dispersed in the collagen solution. The cells can be evaluated after culturing by staining such as with neutral red, or with fluorescein diacetate and irradiating, or by photographing cells in the collagen gel. The support surface can be a continuous ridge forming a recessed area and the drop of collagen solution is placed in the recessed area. Cancer cells can be cultured to test for sensitivity to an anticancer agent. The interaction of cells can be observed in co-culture tests where different kinds of cells are cultured simultaneously. Culturing in a drop enables using a small amount of animal cells sampled from tissue.

摘要翻译： PCT No.PCT / JP94 / 02222 Sec。 371日期1995年8月24日 102（e）日期1995年8月24日PCT 1994年12月26日PCT PCT。 公开号WO95 / 18216 日期1995年7月6日在植入胶原凝胶的同时培养动物细胞。 含有凝胶的细胞通过将动物细胞分散在胶原溶液中，将含有细胞的胶原溶液的滴（或滴）放置在支持体表面上并使滴下凝胶固定在表面上，形成为具有球形胶原凝胶 一个凸面。 通过使凝胶与可以是无血清或含有葡聚糖硫酸盐的培养基接触来培养细胞。 该液滴优选含有约3至约300微升的胶原溶液，并且高度为约2mm或更小。 细胞可以在具有胶原层的支撑表面上进行预培养，胶原层通过用胶原酶处理从胶原层释放并分散在胶原溶液中。 可以通过染色如中性红培养或用荧光素二乙酸酯进行培养来照射细胞，并照射或通过拍摄胶原凝胶中的细胞。 支撑表面可以是形成凹陷区域的连续脊，并且胶原溶液的液滴被放置在凹陷区域中。 可以培养癌细胞以测试对抗癌剂的敏感性。 在同时培养不同种类的细胞的共培养试验中可观察到细胞的相互作用。 在一滴中培养使得能够使用从组织中取样的少量动物细胞。