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    Method for culturing animal cells in collagen drops on a support
    2.
    发明授权
    Method for culturing animal cells in collagen drops on a support 失效
    在支架上培养胶原蛋白中的动物细胞的方法

    公开(公告)号：US5712161A

    公开(公告)日：1998-01-27

    申请号：US507418

    申请日：1995-08-24

    申请人： Masahiro Koezuka ,  Naohito Kondo ,  Hisayuki Kobayashi ,  Hiroshi Saeki ,  Keizo Tanisaka ,  Sachiko Oda

    发明人： Masahiro Koezuka ,  Naohito Kondo ,  Hisayuki Kobayashi ,  Hiroshi Saeki ,  Keizo Tanisaka ,  Sachiko Oda

    IPC分类号： C12N5/00 ,  C12N5/08 ,  C12N1/02 ,  C12N11/04 ,  C12Q1/02

    CPC分类号： C12N5/0012 ,  C12N2533/54

    摘要： Animal cells are cultured while embedded in a collagen gel. The gel containing cells is formed by dispersing animal cells in a collagen solution, placing a drop (or drops) of the cell-containing collagen solution on a support surface and allowing the drop to gel to fix on the surface as a globular collagen gel having a convex surface. The cells are cultured by contacting the gel with a culture medium that may be serum-free or contain dextran sulfate. The drop preferably contains about 3 to about 300 microliters of the collagen solution and is about 2 mm or less in height. The cells may be precultured on a support surface having a collagen layer, released from the collagen layer by treatment with collagenase and dispersed in the collagen solution. The cells can be evaluated after culturing by staining such as with neutral red, or with fluorescein diacetate and irradiating, or by photographing cells in the collagen gel. The support surface can be a continuous ridge forming a recessed area and the drop of collagen solution is placed in the recessed area. Cancer cells can be cultured to test for sensitivity to an anticancer agent. The interaction of cells can be observed in co-culture tests where different kinds of cells are cultured simultaneously. Culturing in a drop enables using a small amount of animal cells sampled from tissue.

    摘要翻译： PCT No.PCT / JP94 / 02222 Sec。 371日期1995年8月24日 102（e）日期1995年8月24日PCT 1994年12月26日PCT PCT。 公开号WO95 / 18216 日期1995年7月6日在植入胶原凝胶的同时培养动物细胞。 含有凝胶的细胞通过将动物细胞分散在胶原溶液中，将含有细胞的胶原溶液的滴（或滴）放置在支持体表面上并使滴下凝胶固定在表面上，形成为具有球形胶原凝胶 一个凸面。 通过使凝胶与可以是无血清或含有葡聚糖硫酸盐的培养基接触来培养细胞。 该液滴优选含有约3至约300微升的胶原溶液，并且高度为约2mm或更小。 细胞可以在具有胶原层的支撑表面上进行预培养，胶原层通过用胶原酶处理从胶原层释放并分散在胶原溶液中。 可以通过染色如中性红培养或用荧光素二乙酸酯进行培养来照射细胞，并照射或通过拍摄胶原凝胶中的细胞。 支撑表面可以是形成凹陷区域的连续脊，并且胶原溶液的液滴被放置在凹陷区域中。 可以培养癌细胞以测试对抗癌剂的敏感性。 在同时培养不同种类的细胞的共培养试验中可观察到细胞的相互作用。 在一滴中培养使得能够使用从组织中取样的少量动物细胞。

    Method for testing the sensitivity of anticancer drug
    3.
    发明授权
    Method for testing the sensitivity of anticancer drug 失效
    抗癌药物敏感性检测方法

    公开(公告)号：US5356793A

    公开(公告)日：1994-10-18

    申请号：US649526

    申请日：1991-02-01

    申请人： Masahiro Koezuka ,  Naohito Kondo ,  Sachiko Oda ,  Hisayuki Kobayashi ,  Masayuki Yasutomi

    发明人： Masahiro Koezuka ,  Naohito Kondo ,  Sachiko Oda ,  Hisayuki Kobayashi ,  Masayuki Yasutomi

    IPC分类号： G01N15/14 ,  G01N33/50 ,  C12M1/00 ,  C12M3/00 ,  C12N5/00 ,  C12N5/06

    CPC分类号： G01N33/5011 ,  G01N15/1475 ,  G01N2800/52 ,  Y10S435/808

    摘要： The present invention relates to a method of testing the sensitivity of cancer drugs with cancer cells cultured in vitro. Cancer cells are cultured in a collagen gel substrate. A wide variety of human cancer cell types readily proliferate in the collagen gel substrate, however, fibroblast cells proliferate as well. The measurement of the growth of the cancer cells is hindered by the presence of the fibroblast cells. The present invention solves this problem by counting the number of colonies with an image processor which selectively extracts the image signals of cancer cells and their colonies. In a second embodiment, the growth of cancer cells is determined by measuring the volume of colonies with the image signals of cancer cells and their colonies selectively extracted. The results can be obtained effectively within a short period of time.

    摘要翻译： 本发明涉及一种检测癌症药物与体外培养的癌细胞的敏感性的方法。 癌细胞在胶原凝胶底物中培养。 各种人类癌细胞类型在胶原凝胶底物中容易增殖，然而，成纤维细胞也增殖。 癌细胞生长的测量被成纤维细胞的存在所阻碍。 本发明通过用选择性提取癌细胞及其菌落的图像信号的图像处理器计数菌落数来解决这个问题。 在第二个实施方案中，癌细胞的生长通过用选择性提取的癌细胞及其菌落的图像信号测量菌落的体积来确定。 结果可以在短时间内有效获得。

    Pad structure of wiring board and wiring board
    4.
    发明申请
    Pad structure of wiring board and wiring board 审中-公开
    接线板和接线板的焊盘结构

    公开(公告)号：US20060209497A1

    公开(公告)日：2006-09-21

    申请号：US10549079

    申请日：2004-09-21

    申请人： Kazuhiko Ooi ,  Kenjiro Enoki ,  Sachiko Oda

    发明人： Kazuhiko Ooi ,  Kenjiro Enoki ,  Sachiko Oda

    IPC分类号： H02B1/00

    CPC分类号： H05K3/244 ,  C23C18/1651 ,  H01L23/49816 ,  H01L2924/0002 ,  H05K3/3457 ,  H05K2203/072 ,  H01L2924/00

    摘要： A pad structure for a circuit board including a phosphorus-containing nickel layer is provided, capable of improving a tensile strength of a solder member such as a solder ball mounted thereon or a foreign member soldered thereto. The pad structure (40) is a multi-layer plated structure provided in a conductor pattern of the substrate, for mounting the solder bump (20) thereon, and formed as part of the conductor pattern, including a metal layer (10) formed as part of the conductor pattern to constitute a pad body, a phosphorus-containing nickel layer (12) formed by an electroless nickel plating to be directly brought into contact with the metal layer, a copper layer (14) thinner than the nickel layer, formed on the nickel layer by an electroless copper plating, and a precious metal layer (16) formed on the copper layer by an electroless precious metal plating.

    摘要翻译： 提供了一种用于包括含磷镍层的电路板的焊盘结构，其能够提高焊接部件（例如安装在其上的焊球）或焊接的外部部件的抗拉强度。 衬垫结构（40）是设置在衬底的导体图案中的多层电镀结构，用于将焊料凸块（20）安装在其上，并形成为导体图案的一部分，包括金属层（10），其形成为 构成衬垫体的导体图案的一部分，通过化学镀镍形成的含磷镍层（12）与金属层直接接触，形成比镍层薄的铜层（14） 通过化学镀铜在镍层上，通过无电镀贵金属镀在铜层上形成的贵金属层（16）。

    Data processing device
    6.
    发明授权
    Data processing device 有权
    数据处理装置

    公开(公告)号：US06647442B1

    公开(公告)日：2003-11-11

    申请号：US09457499

    申请日：1999-12-08

    申请人： Mitsuru Shimada ,  Shinichirou Ikoma ,  Atsushi Takegami ,  Sachiko Oda

    发明人： Mitsuru Shimada ,  Shinichirou Ikoma ,  Atsushi Takegami ,  Sachiko Oda

    IPC分类号： G06F300

    CPC分类号： G01B11/2545

    摘要： To avoid a reduction in the efficiency of data transmission of a data processing device that conducts data communications using a serial bus that conforms to the IEEE 1394 Standards. The data processing device 1 has a calculator 9 and a comparator 8. The calculator 9 calculates the data volume of a response packet intended for reception that corresponds to a request packet intended for transmission when an attempt is made to transmit these packets, the comparator 8 compares the data volume of the response packet intended for reception and the empty volume of the receiving buffer 7, and when the empty volume of the receiving buffer 7 is smaller than the data volume of the response packet intended for reception, the packet transmitting device 3 does not transmits the request packet intended for transmission to the IEEE 1394 bus 6, and if the empty volume of the receiving buffer is too small and the response packet cannot be received, the request packet is not transmitted.

    摘要翻译： 为了避免使用符合IEEE 1394标准的串行总线进行数据通信的数据处理装置的数据传输效率的降低。数据处理装置1具有计算器9和比较器8.计算器9计算 当尝试发送这些分组时，旨在用于接收的响应分组的数据量对应于用于发送的请求分组，比较器8将用于接收的响应分组的数据量与接收缓冲器的空容量进行比较 如图7所示，并且当接收缓冲器7的空容量小于用于接收的响应包的数据量时，分组发送装置3不将要发送的请求包发送到IEEE 1394总线6，并且如果 接收缓冲区的空容量太小，响应报文不能被接收，请求报文不被发送。