摘要:
A process for preparing a low-molecular weight chitosan which comprises adding chitosan to water containing a monobasic acid to prepare an intimate chitosan-water mixture and treating said mixture with cellulase.
摘要:
A process for producing deacetylase which comprises incubating a deacetylase-producing bacterium belonging to Vibrio cholerae in a IFO 15429 culture medium containing a carbon source, a nitrogen source, an inorganic salt and an inducer for producing the deacetylase and isolating the deacetylase from cells separated from said culture medium.
摘要:
There are provided N-acetyl-D-glucosamine deacetylase capable of hydrolyzing acetamide group of N-acetyl-D-glucosamine to produce D-glucosamine and acetic acid, which has the following physicochemical properties:(1) Substrate specificity: N-acetyl-D-glucosamine monomer, not the oligomer or polymer thereof;(2) Optimal pH: 7.8-8.2 at 37.degree. C.;(3) Stable pH: 6.0-9.0 at 45.degree. C.;(4) Optimal temperature: 28.degree.-39.degree. C.;(5) Molecular weight: 70,000-200,000 as determined using TSK-gel G-3000 SW column,and a process for preparing the same using a microorganism belonging to Vibrio sp.