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公开(公告)号：US5063153A
公开(公告)日：1991-11-05
申请号：US107674
申请日：1987-10-09
CPC分类号： C12Q1/32 , G01N33/525
摘要： An improvement of a dry integral multilayer analytical element having functional layers which contain dehydrogenase, oxidized nicotinamide coenzyme, an electron transport compound and an electron acceptable dye-forming compound is disclosed. The improvement the analytical element resides in that an alkali agent or an alkaline buffer is contained in a layer which is different from a layer or layers containing the oxidized nicotinamide coenzyme and electron acceptable dye-forming compound.
摘要翻译： 公开了具有功能层的含有脱氢酶，氧化烟酰胺辅酶，电子传递化合物和电子可接受的染料形成化合物的干整体多层分析元件的改进。 分析元件的改进在于与不同于含有氧化的烟酰胺辅酶和可电镀的染料形成化合物的层的层中含有碱剂或碱性缓冲剂。
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公开(公告)号：US4966784A
公开(公告)日：1990-10-30
申请号：US339015
申请日：1989-04-14
IPC分类号： G01N33/52
CPC分类号： G01N33/526
摘要： A method of preparing an integral multilayer analytical element such as for analysis of calcium comprising a water-impermeable light-transmissive support, a reagent layer containing a water-soluble indicator capable of reacting with an analyte to produce an optically detectable change, and a porous spreading layer containing a spreading action controller and/or an acid capable of decomposing the calcium compounds in a sample, superposed in this order, wherein the spreading action controller and/or the acid is dissolved in an organic solvent which does not dissolve the above water-soluble indicator, this solution is incorporated into the porous spreading layer, and this superposed material is dried. In the method of the present invention, the migration of the water-soluble indicator in the reagent layer at the time of incorporating the spreading action controller or the acid into the spreading layer is inhibited by using a particular organic solvent. Migrations of the indicator and colored material to the spreading layer are also lowered by the spreading action controller, and by these, analytical accuracy of the analytical element is improved. In the case of the analytical element for analysis of calcium, permeation of the acid into other layers than the spreading layer is inhibited, and permeation of a pH buffer in other layer(s) into the spreading layer is also inhibited by using a particular organic solvent. As a result, effective decomposition of calcium compounds in a sample and effective coloration can be achieved.
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3.发明授权
公开(公告)号：US4871679A
公开(公告)日：1989-10-03
申请号：US73759
申请日：1987-07-15
IPC分类号： G01N33/52
CPC分类号： G01N33/526
摘要： A method of preparing an integral multilayer analytical element such as for analysis of calcium comprising a water-impermeable light-transmissive support, a reagent layer containing a water-soluble indicator capable of reacting with an analyte to produce an optically detectable change, and a porous spreading layer containing a spreading action controller and/or an acid capable of decomposing the calcium compounds in a sample, superposed in this order, wherein the spreading action controller and/or the acid is dissolved in an organic solvent which does not dissolve the above water-soluble indicator, this solution is incorporated into the porous spreading layer, and this superposed material is dried. In the method of the present invention, the migration of the water-soluble indicator in the reagent layer at the time of incorporating the spreading action controller or the acid into the spreading layer is inhibited by using a particular organic solvent. Migrations of the indicator and colored material to the spreading layer are also lowered by the spreading action controller, and by these, analytical accuracy of the analytical element is improved. In the case of the analytical element for analysis of calcium, permeation of the acid into other layers than the spreading layer is inhibited, and permeation of a pH buffer in other layer(s) into the spreading layer is also inhibited by using a particular organic solvent. As a result, effective decomposition of calcium compounds in a sample and effective coloration can be achieved.
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公开(公告)号：US4604347A
公开(公告)日：1986-08-05
申请号：US577090
申请日：1984-02-06
CPC分类号： G01N33/525 , G01N31/221 , Y10S435/805
摘要： An analytical element containing at least one reagent for dry analysis of analyte in a liquid sample, which contains a nondiffusive acid and a nondiffusive base in different phases, being capable of adjusting the surrounding pH of the reaction area of at least one reagent under fluid contact therebetween, and said at least one reagent is contained in said nondiffusive acid-containing phase or nondiffusive base-containing phase.
摘要翻译： 包含至少一种用于干燥分析液体样品中的分析物的分析元件，其包含不同相中的非扩散性酸和非扩散性碱，能够调节流体接触下至少一种试剂的反应区域的周围pH值 并且所述至少一种试剂包含在所述非扩散性含酸相或非扩散性含碱相中。
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公开(公告)号：US4895704A
公开(公告)日：1990-01-23
申请号：US117355
申请日：1987-10-27
申请人： Fuminori Arai , Takeshi Igarashi
发明人： Fuminori Arai , Takeshi Igarashi
CPC分类号： G01N33/525 , Y10S435/805
摘要： An integral multilayer analytical element which comprises a water-impermeable light-transmissive support, a hydrophilic layer containing a water-absorptive hydrophilic polymer binder and a spreading layer superposed in this order, and contains at least one reagent capable of reacting with a component of a sample to produce a detectable species capable of detecting by light, which is characterized in that light-scattering particulates in an amount to make light transmittance to 10 to 2.5% are contained in the above hydrophilic layer and/or a layer located on the side of the support therefrom. This element may decrease the influence of a conventional reagent layer up to the extent being negligible without lowering the sensitivity. Precision of the measurement at a low concentration range is also high, and reproducibility of the meaurement is well. Manufacture of this element is easy.
摘要翻译： 一种整体多层分析元件，其包含不透水的透光性支撑体，含有吸水性亲水性聚合物粘合剂的亲水层和依次叠加的扩散层，并且含有至少一种能够与 样品以产生能够被光检测的可检测物质，其特征在于将上述亲水层和/或位于上述亲水层侧的层中含有使光透射率达到10至2.5％的量的光散射微粒 其支撑。 该元件可以降低常规试剂层的影响，直到可忽略的程度，而不降低灵敏度。 在低浓度范围内的测量精度也很高，测量的重现性好。 制造这个元素很容易。
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公开(公告)号：US20090202392A1
公开(公告)日：2009-08-13
申请号：US12366683
申请日：2009-02-06
IPC分类号： B01L3/02
CPC分类号： B01L3/0275 , B01L2300/165
摘要： A pipette tip formed by a polypropylene substrate coated with a water repellent agent. The water repellent agent contains a silicone resin containing at least one specific substance selected from the group consisting of diisononyl phthalate, diisodecyl phthalate, trioctyl trimellitate and poly (1,3-butanediol adipate). The total mass of the specific substance is 1-30 mass % of the silicone resin.
摘要翻译： 由涂覆有防水剂的聚丙烯基材形成的吸头。 防水剂含有含有至少一种选自邻苯二甲酸二异壬基酯，邻苯二甲酸二异癸酯，偏苯三酸三辛酯和己二酸1,3-丁二醇酯中的至少一种特定物质的有机硅树脂。 特定物质的总质量为有机硅树脂的1-30质量％。
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公开(公告)号：US5589347A
公开(公告)日：1996-12-31
申请号：US461766
申请日：1995-06-05
申请人： Fuminori Arai , Takeshi Igarashi
发明人： Fuminori Arai , Takeshi Igarashi
CPC分类号： C12Q1/60 , Y10S435/805
摘要： A multilayer analysis element for determination of total cholesterol is prepared having a light-transmissive water-impermeable support, at least one hydrophilic polymer layer on said support and a spreading layer on said hydrophilic polymer layer(s), and containing in one or more of the layers:(a) at least one enzyme having cholesterol esterase activity,(b) cholesterol oxidase,(c) peroxidase,(d) a color reagent composition, which in the presence of hydrogen peroxide and the peroxidase, produces a color change(e) at least one bile acid compound selected from the group consisting of bile acids, bile acid derivatives and salts of said acids and derivatives, and(f) an alkyl phenoxy polyethoxy ethanol containing in the alkyl group 1 to 20, preferably 7 to 10 carbon atoms, and a polyoxyethylene chain composed of at least 16, preferably 30 to 60 oxyethylene units.
摘要翻译： 制备用于测定总胆固醇的多层分析元件，其具有透光不透水支撑体，所述载体上的至少一个亲水性聚合物层和所述亲水性聚合物层上的铺展层，并且包含一个或多个 所述层：（a）至少一种具有胆固醇酯酶活性的酶，（b）胆固醇氧化酶，（c）过氧化物酶，（d）在过氧化氢和过氧化物酶存在下产生颜色变化的着色剂组合物 e）至少一种胆汁酸化合物，其选自胆汁酸，胆汁酸衍生物和所述酸及其衍生物的盐，和（f）烷基苯氧基聚乙氧基乙醇，其含有在烷基1至20，优选7至10 碳原子和由至少16个，优选30-60个氧乙烯单元组成的聚氧乙烯链。
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公开(公告)号：US4826761A
公开(公告)日：1989-05-02
申请号：US883065
申请日：1986-07-08
申请人： Fuminori Arai , Takeshi Igarashi
发明人： Fuminori Arai , Takeshi Igarashi
CPC分类号： C12Q1/60
摘要： An integral multilayer analytical element for analysis of cholesterol which comprises a water-impermeable light-transmissive support, a hydrophilic layer and a spreading layer superposed in this order, and contains cholesterol esterase, cholesterol oxidase, peroxidase and a coloring reagent composition, which is characterized, in that the above coloring reagent composition is a combination of 4-aminoantipyrine or its derivative and 2-hydroxy-3,5-dichlorobenzene sulfonic acid, and in that pH of the layer where coloring reaction proceeds is kept in the range of 7.5 to 9.This analytical element is stable, and capable of preserving more than 8 months without fogging.
摘要翻译： 用于胆固醇分析的整体多层分析元件，其包含不透水的透光性支持体，亲水层和铺展层，依次叠加，含有胆固醇酯酶，胆固醇氧化酶，过氧化物酶和着色试剂组合物，其特征在于 因为上述着色试剂组合物是4-氨基安替比林或其衍生物与2-羟基-3,5-二氯苯磺酸的组合，并且着色反应进行的层的pH保持在7.5〜 9.这种分析元素是稳定的，能够保存超过8个月而不起雾。
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公开(公告)号：US5215716A
公开(公告)日：1993-06-01
申请号：US667001
申请日：1991-03-11
申请人： Fuminori Arai
发明人： Fuminori Arai
IPC分类号： G01N33/52
CPC分类号： G01N33/525
摘要： An integral multilayer analytical element comprising a reagent layer containing an indicator reagent composition capable of producing a detectable change corresponding to an analyte and a porous spreading layer composed of a fibrous fabric superposed on the reagent layer, wherein the yarn of said fabric comprises fibers having roughed surface, and an integral multilayer analytical element comprising a water-absorptive layer containing a hydrophilic polymer and a porous spreading layer composed of a fibrous fabric containing an indicator reagent composition capable of producing a detectable change corresponding to an analyte superposed on the water-absorptive layer, wherein the yarn of said fabric comprises fibers having roughed surface.The rouging is carried out, for example, by alkali etching.
摘要翻译： 一种整体多层分析元件，其包含试剂层，所述试剂层包含能够产生对应于分析物的可检测变化的指示剂组合物和由叠置在所述试剂层上的纤维织物组成的多孔扩散层，其中所述织物的纱线包含粗糙的纤维 表面和包含含有亲水性聚合物的吸水层和由含有指示剂组合物的纤维织物组成的多孔扩散层的整体多层分析元件，所述指示剂组合物能够产生与叠加在吸水层上的分析物相对应的可检测变化 其中所述织物的纱线包括具有粗糙表面的纤维。 例如，通过碱蚀刻进行粗化。
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公开(公告)号：USH930H
公开(公告)日：1991-06-04
申请号：US321977
申请日：1989-03-10
申请人： Keiko Kato , Fuminori Arai , Harumi Katsuyama
发明人： Keiko Kato , Fuminori Arai , Harumi Katsuyama
CPC分类号： C12Q1/54
摘要： A dry-type quantitative glucose-analyzing element comprising a water-impermeable light-transmissive support having thereon a group of water-permeable layers comprising a reagent layer, an enzyme-containing layer, and a porous spreading layer which are laminated on said support in this order, and a hydrogen peroxide-detecting coloring composition comprising a hydrogen donor and a coupler; whereinsaid reagent layer contains at least said coupler;said enzyme-containing layer contains a glucose oxidase, a peroxidase and a mordant which immobilizes a dye formed by said coloring composition; andsaid hydrogen donor is contained in at least one water-permeable layer of said group. Using the element, quantitative determination of glucose of high concentration is possible with high accuracy.
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