公开(公告)号：US20150144492A1

公开(公告)日：2015-05-28

申请号：US14403598

申请日：2013-05-31

Applicant: GE HEALTHCARE BIO-SCIENCES AB

Inventor： Lennart Bjorkesten ,  Owe Salven ,  Camilla Larsson ,  Erik J Bjerneld ,  Sofia Edlund ,  Henrik Ostlin ,  Stefan Sjolander ,  Kajsa Stridsberg-Friden ,  Ola Ronn ,  Peter Oliviusson ,  HENRIK Bjorkman

IPC: G01N27/447

CPC classification number: G01N27/44743 ,  B01L9/527 ,  G01N27/44704 ,  G01N27/44756

Abstract: Electrophoresis gel cassette comprising a first and a second face wall member and one or more side wall members, defining a gel compartment for a gel member with a first and second face, wherein the first face wall member has high gel adhesion compared to the second face wall member, whereby a gel member molded in the cassette will stay attached to the high gel adhesion face wall member when the cassette is opened, and wherein the first face wall member is provided with at least one removable section to expose a section of the first face of the gel member, the removable section of the first face wall member having lower gel adhesion compared to the non-removable part of the first face wall member.

Abstract translation: 电泳凝胶盒，包括第一和第二面壁构件和一个或多个侧壁构件，其限定具有第一和第二面的凝胶构件的凝胶室，其中第一面壁构件与第二面相比具有高凝胶粘附力 其中当所述盒被打开时，模制在所述盒中的凝胶构件将保持附着到所述高凝胶粘附面壁构件，并且其中所述第一面壁构件设置有至少一个可移除部分，以暴露所述第一 凝胶构件的表面，与第一面壁构件的不可移除部分相比，第一面壁构件的可移除部分具有较低的凝胶粘附。

公开(公告)号：US20140106373A1

公开(公告)日：2014-04-17

申请号：US14122382

申请日：2012-05-29

Applicant: GE HEALTHCARE BIO-SCIENCES AB

Inventor： Asa Hagner-Mcwhirter ,  Ola Ronn

IPC: G01N33/566

CPC classification number: G01N33/566 ,  C07K1/26 ,  G01N27/44726

Abstract: The present invention relates to a method for target protein normalization, especially for Western blotting applications. More closely, the invention relates to a method for normalizing target protein signals, after electrophoresis and Western blotting, against variations of sample load or cell number between different lanes or within the same lane on an electrophoretic gel. The signals are normalized against the total protein signal (=ratio between target protein/total protein) or reference protein band signal(s) (=ratio between target protein/reference protein band).According to the invention multiplex and quantitative assessments are possible, such as quantitative comparison between target proteins in different samples.

Abstract translation: 本发明涉及一种用于靶蛋白标准化的方法，特别是用于Western印迹应用。 更具体地说，本发明涉及一种用于在电泳和Western印迹之后对靶蛋白信号进行标准化的方法，其针对电泳凝胶上不同泳道之间或同一泳道内的样品载量或细胞数量的变化。 信号相对于总蛋白信号（=靶蛋白/总蛋白之间的比例）或参考蛋白质条带信号（=靶蛋白质/参考蛋白质条带之间的比率）进行归一化。 根据本发明，可以进行多重和定量评估，例如不同样品中靶蛋白的定量比较。

公开(公告)号：US09383335B2

公开(公告)日：2016-07-05

申请号：US14403613

申请日：2013-05-31

Applicant: GE HEALTHCARE BIO-SCIENCES AB

Inventor： Lennart Bjorkesten ,  Owe Salven ,  Camilla Larsson ,  Erik J Bjerneld ,  Sofia Edlund ,  Henrik Ostlin ,  Stefan Sjolander ,  Kajsa Stridsberg-Friden ,  Ola Ronn ,  Peter Oliviusson ,  Henrik Bjorkman

IPC: G01N27/453 ,  G01N27/447

CPC classification number: G01N27/447 ,  G01N27/44704 ,  G01N27/44756

Abstract: Electrophoresis gel cassette comprising a first and a second face wall member and one or more side wall members defining a gel compartment for a gel member with a first and second face, wherein at least a section of each one of the first and second face wall member is removable to expose a section of both the first and second face of the gel member essentially corresponding to the separation zone while the gel member is preserved in the cassette.

Abstract translation: 电泳凝胶盒包括第一和第二面壁构件和一个或多个侧壁构件，所述侧壁构件限定具有第一和第二面的凝胶构件的凝胶室，其中，所述第一和第二面壁构件中的每一个的至少一部分 可拆卸以暴露凝胶构件的第一面和第二面的基本上对应于分离区的部分，同时将凝胶构件保存在盒中。

公开(公告)号：US20150185181A1

公开(公告)日：2015-07-02

申请号：US14403613

申请日：2013-05-31

Applicant: GE HEALTHCARE BIO-SCIENCES AB

Inventor： Lennart Bjorkesten ,  Owe Salven ,  Camilla Larsson ,  Erik J. Bjerneld ,  Sofia Edlund ,  Henrik Ostlin ,  Stefan Sjolander ,  Kajsa Stridsberg-Friden ,  Ola Ronn ,  Peter Oliviusson ,  Henrik Bjorkman

IPC: G01N27/447

CPC classification number: G01N27/447 ,  G01N27/44704 ,  G01N27/44756

Abstract: Electrophoresis gel cassette comprising a first and a second face wall member and one or more side wall members defining a gel compartment for a gel member with a first and second face, wherein at least a section of each one of the first and second face wall member is removable to expose a section of both the first and second face of the gel member essentially corresponding to the separation zone while the gel member is preserved in the cassette.

Abstract translation: 电泳凝胶盒包括第一和第二面壁构件和一个或多个侧壁构件，所述侧壁构件限定具有第一和第二面的凝胶构件的凝胶室，其中，所述第一和第二面壁构件中的每一个的至少一部分 可拆卸以暴露凝胶构件的第一面和第二面的基本上对应于分离区的部分，同时将凝胶构件保存在盒中。

公开(公告)号：US20150177186A1

公开(公告)日：2015-06-25

申请号：US14403593

申请日：2013-05-31

Applicant: GE HEALTHCARE BIO-SCIENCES AB

Inventor： Lennart Bjorkesten ,  Owe Salven ,  Camilla Larsson ,  Erik J. Bjerneld ,  Sofia Edlund ,  Henrik Ostlin ,  Stefan Sjolander ,  Kajsa Stridsberg-Friden ,  Ola Ronn ,  Peter Oliviusson ,  Henrik Bjorkman

IPC: G01N27/447

CPC classification number: G01N27/447 ,  G01N27/44704

Abstract: Electrophoresis gel unit comprising a flat gel member with an upper and a lower face and a sample separation zone, wherein the gel member is attached to a rigid support arranged to preserve the shape of and to facilitate handling of the gel member, wherein the rigid support is formed to allow access to a section of both the upper and lower face of the gel member essentially corresponding to the separation zone.

Abstract translation: 电泳凝胶单元包括具有上表面和下表面的平坦凝胶构件和样品分离区，其中所述凝胶构件附接到刚性支撑件，所述刚性支撑件设置成保持凝胶构件的形状和便于处理凝胶构件，其中刚性支撑件 被形成为允许进入基本上对应于分离区的凝胶构件的上表面和下表面的一部分。