公开(公告)号：US10308690B2

公开(公告)日：2019-06-04

申请号：US14961164

申请日：2015-12-07

Applicant: GE HEALTHCARE BIOPROCESS R&D AB

Inventor： Gustav Rodrigo ,  Mats Ander ,  Göran Baurén ,  Tomas Björkman

IPC: C07K14/31 ,  B01J20/32 ,  B01D15/38 ,  C07K1/22 ,  B01J20/286 ,  C07K14/745 ,  C07K16/00

Abstract: The invention discloses a polypeptide with improved alkaline stability, which polypeptide comprises a mutant of a B or C domain of Staphylococcus Protein A (SpA), as specified by SEQ ID NO 1 or SEQ ID NO 2, or of Protein Z, as specified by SEQ ID NO 3, comprising at least the mutation wherein the glutamine residue at position 9 has been mutated to a tryptophan, leucine, glutamic acid, valine or lysine. The invention also discloses multimers of the polypeptide, as well as separation matrices comprising the multimers or polypeptides.