公开(公告)号：US20200023338A1

公开(公告)日：2020-01-23

申请号：US16337236

申请日：2017-09-27

Applicant: GE Healthcare BioProcess R&D AB

Inventor： Ann Catharina Bergh ,  Tania Ahmad ,  Mattias Algotsson ,  Therese Graner

IPC: B01J20/289 ,  B01D15/38 ,  B01J20/28 ,  B01J20/32

Abstract: The present invention relates to a novel chromatography media, more closely a novel IMAC (Immobilized Metal Affinity Chromatography) media. The novel chromatography media comprises a pentaligand and provides high dynamic binding capacity as well as high purity of the sample proteins purified on the media of the invention.

公开(公告)号：US10662261B2

公开(公告)日：2020-05-26

申请号：US15937428

申请日：2018-03-27

Applicant: GE HEALTHCARE BIOPROCESS R&D AB

Inventor： Mattias Algotsson ,  Lars C. Andersson ,  Eggert Brekkan ,  Lee Hedemyr ,  Jean-Luc Maloisel ,  Ronnie Palmgren

IPC: C08B37/00 ,  B01J20/289 ,  B01J20/32 ,  C07C237/10 ,  C07C323/60 ,  B01J20/281

Abstract: The present invention relates to dimeric pentadentate chelators with exceptionally strong binding of metal ions, for detection, immobilization and purification of biomolecules. Dimeric chelators offer a cooperativity of binding of two adjacent immobilized metal ions simultaneously to a histidine-tagged biomolecule, which gives advantageous properties regarding strength of binding compared to a corresponding monomer chelator. In addition, a dimer increases the selectivity (ease of separation) against non-tagged biomolecules with low metal-ion affinity.

公开(公告)号：US20180223003A1

公开(公告)日：2018-08-09

申请号：US15937428

申请日：2018-03-27

Applicant: GE HEALTHCARE BIOPROCESS R&D AB

Inventor： Mattias Algotsson ,  Lars C. Andersson ,  Eggert Brekkan ,  Lee Hedemyr ,  Jean-Luc Maloisel ,  Ronnie Palmgren

IPC: C08B37/00 ,  C07C323/60 ,  C07C237/10 ,  B01J20/32 ,  B01J20/281 ,  B01J20/289

CPC classification number: C08B37/0039 ,  B01J20/281 ,  B01J20/289 ,  B01J20/3219 ,  B01J20/3251 ,  B01J20/3265 ,  C07C237/10 ,  C07C323/60

Abstract: The present invention relates to dimeric pentadentate chelators with exceptionally strong binding of metal ions, for detection, immobilization and purification of biomolecules. Dimeric chelators offer a cooperativity of binding of two adjacent immobilized metal ions simultaneously to a histidine-tagged biomolecule, which gives advantageous properties regarding strength of binding compared to a corresponding monomer chelator. In addition, a dimer increases the selectivity (ease of separation) against non-tagged biomolecules with low metal-ion affinity.

公开(公告)号：US20180304231A1

公开(公告)日：2018-10-25

申请号：US15769400

申请日：2016-10-28

Applicant: GE HEALTHCARE BIOPROCESS R&D AB

Inventor： Nils Norrman ,  Mattias Algotsson ,  Stefan D. Eriksson

IPC: B01J20/24 ,  B01J20/28 ,  B01J20/29 ,  B01J20/30 ,  C02F1/42

CPC classification number: B01J20/24 ,  B01J20/28016 ,  B01J20/29 ,  B01J20/305 ,  C02F1/42

Abstract: The present invention relates to a method to improve chromatography beads. More closely, the invention relates to a novel method for production of dextran-containing porous media and chromatography media produced with this method. In the method, the chromatography media is subjected to dextranase-treatment leading to improved pressure-flow properties of the media.