公开(公告)号：US11732291B2

公开(公告)日：2023-08-22

申请号：US16849857

申请日：2020-04-15

Applicant: GEN-PROBE INCORPORATED

Inventor： James Carlson ,  Reinhold Pollner ,  Steven T. Brentano

IPC: C07H21/04 ,  C12Q1/6834 ,  C12Q1/6816

CPC classification number: C12Q1/6834 ,  C12Q1/6816 ,  C12Q1/6816 ,  C12Q2525/301 ,  C12Q2527/107 ,  C12Q2565/501

Abstract: The invention provides an improved stem-loop target capture oligomer and methods of use. Such a target capture oligomer has a target-binding segment forming a loop flanked by stem segments forming a stem. The stem segments are of unequal length. Such probes show little or no binding to immobilized probes in the absence of a target nucleic acid but offer good target sensitivity. The probes are particularly useful in multiplex methods of detection in which multiple target capture oligomers are present for detecting of multiple target nucleic acids (for example, detecting multiple polymorphic forms of a target gene).

公开(公告)号：US20200248246A1

公开(公告)日：2020-08-06

申请号：US16849857

申请日：2020-04-15

Applicant: GEN-PROBE INCORPORATED

Inventor： James Carlson ,  Reinhold Pollner ,  Steven T. Brentano

IPC: C12Q1/6834 ,  C12Q1/6816

Abstract: The invention provides an improved stem-loop target capture oligomer and methods of use. Such a target capture oligomer has a target-binding segment forming a loop flanked by stem segments forming a stem. The stem segments are of unequal length. Such probes show little or no binding to immobilized probes in the absence of a target nucleic acid but offer good target sensitivity. The probes are particularly useful in multiplex methods of detection in which multiple target capture oligomers are present for detecting of multiple target nucleic acids (for example, detecting multiple polymorphic forms of a target gene).

公开(公告)号：US10655165B2

公开(公告)日：2020-05-19

申请号：US14376128

申请日：2013-02-01

Applicant: GEN-PROBE INCORPORATED

Inventor： James Carlson ,  Reinhold Pollner ,  Steven T. Brentano

IPC: C12Q1/68 ,  C12Q1/6834 ,  C12Q1/6816

Abstract: The invention provides an improved stem-loop target capture oligomer and methods of use. Such a target capture oligomer has a target-binding segment forming a loop flanked by stem segments forming a stem. The stem segments are of unequal length. Such probes show little or no binding to immobilized probes in the absence of a target nucleic acid but offer good target sensitivity. The probes are particularly useful in multiplex methods of detection in which multiple target capture oligomers are present for detecting of multiple target nucleic acids (for example, detecting multiple polymorphic forms of a target gene).

公开(公告)号：US20140370506A1

公开(公告)日：2014-12-18

申请号：US14376128

申请日：2013-02-01

Applicant: GEN-PROBE INCORPORATED

Inventor： James Carlson ,  Reinhold Pollner ,  Steven T. Brentano

IPC: C12Q1/68

CPC classification number: C12Q1/6834 ,  C12Q1/6816 ,  C12Q2525/301 ,  C12Q2527/107 ,  C12Q2565/501

Abstract: The invention provides an improved stem-loop target capture oligomer and methods of use. Such a target capture oligomer has a target-binding segment forming a loop flanked by stem segments forming a stem. The stem segments are of unequal length. Such probes show little or no binding to immobilized probes in the absence of a target nucleic acid but offer good target sensitivity. The probes are particularly useful in multiplex methods of detection in which multiple target capture oligomers are present for detecting of multiple target nucleic acids (for example, detecting multiple polymorphic forms of a target gene).

Abstract translation: 本发明提供了改进的茎 - 环目标捕获寡聚体和使用方法。 这种靶捕获低聚物具有靶结合段，其形成两侧为形成茎的茎段的环。 茎段长度不等。 这样的探针在没有靶核酸的情况下显示很少或没有结合固定化的探针，但提供良好的靶灵敏度。 探针在多重检测寡核苷酸多重检测方法中特别有用，用于检测多种靶核酸（例如，检测靶基因的多态多态性）。