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    ASYMMETRIC HAIRPIN TARGET CAPTURE OLIGOMERS
    1.
    发明申请
    ASYMMETRIC HAIRPIN TARGET CAPTURE OLIGOMERS 审中-公开

    公开(公告)号：US20200248246A1

    公开(公告)日：2020-08-06

    申请号：US16849857

    申请日：2020-04-15

    Applicant: GEN-PROBE INCORPORATED

    Inventor： James Carlson ,  Reinhold Pollner ,  Steven T. Brentano

    IPC: C12Q1/6834 ,  C12Q1/6816

    Abstract: The invention provides an improved stem-loop target capture oligomer and methods of use. Such a target capture oligomer has a target-binding segment forming a loop flanked by stem segments forming a stem. The stem segments are of unequal length. Such probes show little or no binding to immobilized probes in the absence of a target nucleic acid but offer good target sensitivity. The probes are particularly useful in multiplex methods of detection in which multiple target capture oligomers are present for detecting of multiple target nucleic acids (for example, detecting multiple polymorphic forms of a target gene).

    Integrated capture and amplification of target nucleic acid for sequencing
    3.
    发明授权
    Integrated capture and amplification of target nucleic acid for sequencing 有权

    公开(公告)号：US09677122B2

    公开(公告)日：2017-06-13

    申请号：US15018240

    申请日：2016-02-08

    Applicant: GEN-PROBE INCORPORATED

    Inventor： Reinhold Pollner ,  Shyun-Shyun Lee

    IPC: C12P19/34 ,  C12Q1/68 ,  C12Q1/70

    CPC classification number: C12Q1/6806 ,  C12P19/34 ,  C12Q1/6834 ,  C12Q1/686 ,  C12Q1/6874 ,  C12Q1/70 ,  C12Q2531/113 ,  C12Q2565/537

    Abstract: The invention provides efficient methods of preparing a target nucleic acid in a form suitable for sequencing. The methods are particularly amenable for preparing high quality nucleic acids for massively parallel sequencing. The methods involve capturing a target nucleic acid from a sample and PCR amplification of the target nucleic acid. The target nucleic acid is captured by binding to a capture probe, which in turn binds to an immobilized probe. The immobilized probe is typically immobilized via a magnetic bead. The captured target nucleic acid is PCR amplified by thermocycling without prior dissociation of the target nucleic acid from the beads. The efficiency of the method lies in part in that both the capture and amplification steps are performed in a single vessel. The amplified nucleic acid can then be sequenced.

    Asymmetric hairpin target capture oligomers
    4.
    发明授权
    Asymmetric hairpin target capture oligomers 有权

    公开(公告)号：US10655165B2

    公开(公告)日：2020-05-19

    申请号：US14376128

    申请日：2013-02-01

    Applicant: GEN-PROBE INCORPORATED

    Inventor： James Carlson ,  Reinhold Pollner ,  Steven T. Brentano

    IPC: C12Q1/68 ,  C12Q1/6834 ,  C12Q1/6816

    Abstract: The invention provides an improved stem-loop target capture oligomer and methods of use. Such a target capture oligomer has a target-binding segment forming a loop flanked by stem segments forming a stem. The stem segments are of unequal length. Such probes show little or no binding to immobilized probes in the absence of a target nucleic acid but offer good target sensitivity. The probes are particularly useful in multiplex methods of detection in which multiple target capture oligomers are present for detecting of multiple target nucleic acids (for example, detecting multiple polymorphic forms of a target gene).

    ASYMMETRIC HAIRPIN TARGET CAPTURE OLIGOMERS
    5.
    发明申请
    ASYMMETRIC HAIRPIN TARGET CAPTURE OLIGOMERS 审中-公开
    不对称的毛发目标捕获低聚物

    公开(公告)号：US20140370506A1

    公开(公告)日：2014-12-18

    申请号：US14376128

    申请日：2013-02-01

    Applicant: GEN-PROBE INCORPORATED

    Inventor： James Carlson ,  Reinhold Pollner ,  Steven T. Brentano

    IPC: C12Q1/68

    CPC classification number: C12Q1/6834 ,  C12Q1/6816 ,  C12Q2525/301 ,  C12Q2527/107 ,  C12Q2565/501

    Abstract: The invention provides an improved stem-loop target capture oligomer and methods of use. Such a target capture oligomer has a target-binding segment forming a loop flanked by stem segments forming a stem. The stem segments are of unequal length. Such probes show little or no binding to immobilized probes in the absence of a target nucleic acid but offer good target sensitivity. The probes are particularly useful in multiplex methods of detection in which multiple target capture oligomers are present for detecting of multiple target nucleic acids (for example, detecting multiple polymorphic forms of a target gene).

    Abstract translation: 本发明提供了改进的茎 - 环目标捕获寡聚体和使用方法。 这种靶捕获低聚物具有靶结合段，其形成两侧为形成茎的茎段的环。 茎段长度不等。 这样的探针在没有靶核酸的情况下显示很少或没有结合固定化的探针，但提供良好的靶灵敏度。 探针在多重检测寡核苷酸多重检测方法中特别有用，用于检测多种靶核酸（例如，检测靶基因的多态多态性）。

    Capture probes immobilizable via L-nucleotide tail
    6.
    发明授权
    Capture probes immobilizable via L-nucleotide tail 有权

    公开(公告)号：US11035012B2

    公开(公告)日：2021-06-15

    申请号：US15904232

    申请日：2018-02-23

    Applicant: GEN-PROBE INCORPORATED

    Inventor： Reinhold Pollner ,  Mehrdad Majlessi ,  Susan Yamagata ,  Michael M. Becker ,  Mark Reynolds ,  Lyle Arnold

    IPC: C12Q1/68 ,  C07H21/00 ,  C12Q1/70 ,  C12Q1/6837

    Abstract: The invention provides chimeric capture probes immobilizable via an L-nucleic acid tail that can bind to a complementary L-nucleic acid in an immobilized probe. The capture probes are useful for capturing a target nucleic acid from a sample. The L-nucleic acid in the tail of the capture probe bind to the complementary L-nucleic acid in the immobilized probe with similar affinity as would otherwise equivalent D-nucleic acids. However, the L-nucleic acid of the capture probe tail and immobilized probes do not form stable duplexes with D-nucleic acids present in the in the sample containing the target nucleic acid. Binding of nucleic acids in the sample directly to immobilized probe or to the tail of the capture probe is reduced or eliminated increasing the sensitivity and/or specificity of the assay.

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