公开(公告)号：US20180340157A1

公开(公告)日：2018-11-29

申请号：US15989109

申请日：2018-05-24

Applicant: Genia Technologies, Inc.

Inventor： Helen FRANKLIN ,  Cynthia CECH ,  Timothy Kellogg CRAIG ,  Aruna AYER ,  Kirti DHIMAN ,  Natalie B. CHECHELSKI JOHNSTON ,  Joshua N. MABRY ,  Arkadiusz BIBILLO ,  Peter CRISALLI ,  Randall W. DAVIS

IPC: C12N9/12

Abstract: Disclosed are methods for isolating polymerase complexes from a mixture of polymerase complex components. The polymerase complexes can comprise a nanopore to provide isolated nanopore sequencing complexes. The methods relate to the positive and negative isolation of the polymerase complexes and/or nanopore sequencing complexes. Also disclosed is a nucleic acid adaptor for isolating active polymerase complexes, polymerase complexes comprising the nucleic acid adaptor, and methods for isolating active polymerase complexes using the nucleic acid adaptor.

公开(公告)号：US20190119745A1

公开(公告)日：2019-04-25

申请号：US16238874

申请日：2019-01-03

Applicant: GENIA TECHNOLOGIES, INC.

Inventor： Timothy Kellogg CRAIG ,  Cynthia Ann CECH ,  Michael DORWART ,  Liv Elisabeth JENSEN ,  Marshall Winston PORTER ,  Christos TZITZILONIS ,  Alexander Hyun-min YANG

IPC: C12Q1/6869 ,  C07K14/31

CPC classification number: C12Q1/6869 ,  C07K14/31 ,  C12Q2565/607 ,  C12Q2565/631

Abstract: Described herein are engineered alpha-hemolysin subunits having mutated oligomerization domains for assembling into heptameric nanopores in lipid bilayers.

公开(公告)号：US20170088890A1

公开(公告)日：2017-03-30

申请号：US15274770

申请日：2016-09-23

Applicant: Genia Technologies, Inc.

Inventor： Timothy Kellogg CRAIG ,  Cynthia Ann CECH ,  Michael DORWART ,  Liv Elisabeth JENSEN ,  Marshall Winston PORTER ,  Christos TZITZILONIS ,  Alexander Hyun-min YANG

IPC: C12Q1/68 ,  C07K14/31

CPC classification number: C12Q1/6869 ,  C07K14/31 ,  C12Q2565/607 ,  C12Q2565/631

Abstract: Described herein are engineered alpha-hemolysin subunits having mutated oligomerization domains for assembling into heptameric nanopores in lipid bilayers.