公开(公告)号：US20200068815A1

公开(公告)日：2020-03-05

申请号：US16674209

申请日：2019-11-05

申请人： GRAPE KING BIO LTD.

发明人： Pei-Shan Liu ,  Chien-Chih Chen ,  Chin-Chu Chen ,  Li-Ya Lee ,  Wan-Ping Chen ,  Ting-Wei Lin ,  Jui-Hsia Hsu ,  Wei-Ching Chu

IPC分类号： A01G18/00 ,  A61K9/14 ,  A61K31/343 ,  A61K36/07 ,  C12P17/18 ,  C12R1/645 ,  C07D493/06

摘要： The present invention is related to an active substance of Hericium erinaceus having a pain-relieving effect, and a pharmaceutical composition including the active substance. The active substance is prepared using the following steps: (a) inoculating a mycelium of H. erinaceus on an agar plate and incubating at 15-32° C. for 8-16 days; (b) inoculating the incubated H. erinaceus mycelia from step (a) into a medium in a flask and incubating at 20-30° C. and pH 4.5-6.5 for 3-5 days; (c) inoculating the incubated H. erinaceus mycelia from step (b) into a medium in a fermentation tank and incubating at 24-32° C. and pH 4.5-5.5 for 8-16 days to obtain a fermented medium of the H. erinaceus mycelia; and (d) desiccating the fermented medium of the H. erinaceus mycelia from step (c) to obtain the powder of the H. erinaceus mycelia, which is further purified and isolated to obtain a novel compound of H. erinaceus.

公开(公告)号：US11648233B2

公开(公告)日：2023-05-16

申请号：US16674209

申请日：2019-11-05

申请人： GRAPE KING BIO LTD.

发明人： Pei-Shan Liu ,  Chien-Chih Chen ,  Chin-Chu Chen ,  Li-Ya Lee ,  Wan-Ping Chen ,  Ting-Wei Lin ,  Jui-Hsia Hsu ,  Wei-Ching Chu

IPC分类号： A61K31/343 ,  C07D493/06 ,  C12N1/14 ,  A61K9/14 ,  A61K36/07 ,  C12P17/18 ,  C12R1/645

CPC分类号： A61K31/343 ,  A61K9/14 ,  A61K36/07 ,  C07D493/06 ,  C12N1/145 ,  C12P17/181 ,  A61K2236/11 ,  A61K2236/33 ,  A61K2236/331 ,  A61K2236/53 ,  C12R2001/645

摘要： The present invention is related to an active substance of Hericium erinaceus having a pain-relieving effect, and a pharmaceutical composition including the active substance. The active substance is prepared using the following steps: (a) inoculating a mycelium of H. erinaceus on an agar plate and incubating at 15-32° C. for 8-16 days; (b) inoculating the incubated H. erinaceus mycelia from step (a) into a medium in a flask and incubating at 20-30° C. and pH 4.5-6.5 for 3-5 days; (c) inoculating the incubated H. erinaceus mycelia from step (b) into a medium in a fermentation tank and incubating at 24-32° C. and pH 4.5-5.5 for 8-16 days to obtain a fermented medium of the H. erinaceus mycelia; and (d) desiccating the fermented medium of the H. erinaceus mycelia from step (c) to obtain the powder of the H. erinaceus mycelia, which is further purified and isolated to obtain a novel compound of H. erinaceus.

公开(公告)号：US10405504B2

公开(公告)日：2019-09-10

申请号：US15545087

申请日：2016-01-04

申请人： GRAPE KING BIO LTD.

发明人： Yin-Ching Chan ,  Chin-Chu Chen ,  Li-Ya Lee ,  Wan-Ping Chen

IPC分类号： A01N63/00 ,  A01G18/00 ,  A61K36/07 ,  C12M1/00 ,  C12M1/12 ,  C12M1/42 ,  C12N1/14 ,  C12N5/00

摘要： The present invention discloses the active substances for preventing hearing deterioration, its preparation method, the pharmaceutical composition containing the active substances, and the preparation method of the pharmaceutical composition. The preparation method of the active substances is performed by plate cultivation, flask cultivation and fermentation tank cultivation, to obtain the active substances of Hericium erinaceus mycelia in powder form. The powder of H. erinaceus mycelia is proved to have the effect of preventing hearing deterioration.

公开(公告)号：US20180007836A1

公开(公告)日：2018-01-11

申请号：US15545087

申请日：2016-01-04

申请人： GRAPE KING BIO LTD.

发明人： Yin-Ching Chan ,  Chin-Chu Chen ,  Li-Ya Lee ,  Wan-Ping Chen

IPC分类号： A01G1/04 ,  C12M1/00 ,  C12M1/12 ,  C12N1/14 ,  A61K36/07 ,  C12M1/42 ,  C12N5/00

CPC分类号： A01G18/00 ,  A61K36/07 ,  A61K2236/11 ,  C12M1/005 ,  C12M25/06 ,  C12M35/04 ,  C12N1/14 ,  C12N5/0025 ,  C12N2533/76

摘要： The present invention discloses the active substances for preventing hearing deterioration, its preparation method, the pharmaceutical composition containing the active substances, and the preparation method of the pharmaceutical composition. The preparation method of the active substances is performed by plate cultivation, flask cultivation and fermentation tank cultivation, to obtain the active substances of Hericium erinaceus mycelia in powder form. The powder of H. erinaceus mycelia is proved to have the effect of preventing hearing deterioration.

公开(公告)号：US20170312247A1

公开(公告)日：2017-11-02

申请号：US15508369

申请日：2015-09-02

申请人： GRAPE KING BIO LTD.

发明人： Pei-Shan Liu ,  Chien-Chih Chen ,  Chin-Chu Chen ,  Li-Ya Lee ,  Wan-Ping Chen ,  Ting-Wei Lin ,  Jui-Hsia Hsu ,  Wei-Ching Chu

IPC分类号： A61K31/343 ,  C12P17/18 ,  A61K9/14 ,  A61K36/07

CPC分类号： A01G18/00 ,  A61K9/14 ,  A61K31/343 ,  A61K36/07 ,  A61K2236/11 ,  A61K2236/33 ,  A61K2236/331 ,  A61K2236/53 ,  C07D493/06 ,  C12P17/181 ,  C12R1/645

摘要： The present invention is related to an active substance of Hericium erinaceus having a pain-relieving effect, and a pharmaceutical composition including the active substance. The active substance is prepared using the following steps: (a) inoculating a mycelium of H. erinaceus on an agar plate and incubating at 15-32° C. for 8-16 days; (b) inoculating the incubated H. erinaceus mycelia from step (a) into a medium in a flask and incubating at 20-30° C. and pH 4.5-6.5 for 3-5 days; (c) inoculating the incubated H. erinaceus mycelia from step (b) into a medium in a fermentation tank and incubating at 24-32° C. and pH 4.5-5.5 for 8-16 days to obtain a fermented medium of the H. erinaceus mycelia; and (d) desiccating the fermented medium of the H. erinaceus mycelia from step (c) to obtain the powder of the H. erinaceus mycelia, which is further purified and isolated to obtain a novel compound of H. erinaceus.