公开(公告)号：US20130295670A1

公开(公告)日：2013-11-07

申请号：US13944979

申请日：2013-07-18

申请人： Gary E. Wise ,  Shaomian Yao

发明人： Gary E. Wise ,  Shaomian Yao

IPC分类号： C12N5/071

CPC分类号： C12N5/0664 ,  C12N5/0081 ,  C12N2500/02 ,  C12N2500/60 ,  C12N2501/06 ,  C12N2523/00

摘要： Subjecting a heterogeneous cell population (one with both stem cells and non-stem cells) to extreme stress selectively eliminated the non-stem cells and resulted in the enrichment of stem cells in the population. The stress can take many forms, including without limitation, cell toxins, high temperature, high salt, and low oxygen (hypoxic) conditions. The number of stem cells remaining after stress were increased, and showed increased expression of traditional stem cell markers. The stem cells were shown to be capable of proliferation and differentiation into multiple types of cells. This method allows purification of stem cells from adult heterogeneous cell populations on a large scale basis without requirement of expensive equipment, and without requiring the presence of cell surface markers. Stem cells produced by the above method can be used for clinical applications, including tissue engineering.

摘要翻译： 使非均质细胞群体（一个具有干细胞和非干细胞两者）对极端应激选择性地消除非干细胞，并导致群体中干细胞的富集。 压力可以有多种形式，包括但不限于细胞毒素，高温，高盐和低氧（缺氧）条件。 应激后残留的干细胞数量增加，传统干细胞标志物表达增加。 显示干细胞能够增殖和分化成多种类型的细胞。 该方法允许大规模从成体异质细胞群体纯化干细胞，而不需要昂贵的设备，并且不需要细胞表面标记物的存在。 通过上述方法制备的干细胞可用于临床应用，包括组织工程。

公开(公告)号：US09279105B2

公开(公告)日：2016-03-08

申请号：US13944979

申请日：2013-07-18

申请人： Gary E. Wise ,  Shaomian Yao

发明人： Gary E. Wise ,  Shaomian Yao

IPC分类号： C12N5/00 ,  A01N1/02 ,  C12N5/0775

CPC分类号： C12N5/0664 ,  C12N5/0081 ,  C12N2500/02 ,  C12N2500/60 ,  C12N2501/06 ,  C12N2523/00

摘要： Subjecting a heterogeneous cell population (one with both stem cells and non-stem cells) to extreme stress selectively eliminated the non-stem cells and resulted in the enrichment of stem cells in the population. The stress can take many forms, including without limitation, cell toxins, high temperature, high salt, and low oxygen (hypoxic) conditions. The number of stem cells remaining after stress were increased, and showed increased expression of traditional stem cell markers. The stem cells were shown to be capable of proliferation and differentiation into multiple types of cells. This method allows purification of stem cells from adult heterogeneous cell populations on a large scale basis without requirement of expensive equipment, and without requiring the presence of cell surface markers. Stem cells produced by the above method can be used for clinical applications, including tissue engineering.

公开(公告)号：US08501397B2

公开(公告)日：2013-08-06

申请号：US12842754

申请日：2010-07-23

申请人： Gary E. Wise ,  Shaomian Yao

发明人： Gary E. Wise ,  Shaomian Yao

IPC分类号： A01N1/02 ,  C12N15/85

CPC分类号： C12N5/0664 ,  C12N5/0081 ,  C12N2500/02 ,  C12N2500/60 ,  C12N2501/06 ,  C12N2523/00

摘要： Subjecting a heterogeneous cell population (one with both stem cells and non-stem cells) to extreme stress selectively eliminated the non-stem cells and resulted in the enrichment of stem cells in the population. The stress can take many forms, including without limitation, cell toxins, high temperature, high salt, and low oxygen (hypoxic) conditions. The number of stem cells remaining after stress were increased, and showed increased expression of traditional stem cell markers. The stem cells were shown to be capable of proliferation and differentiation into multiple types of cells. This method allows purification of stem cells from adult heterogeneous cell populations on a large scale basis without requirement of expensive equipment, and without requiring the presence of cell surface markers. Stem cells produced by the above method can be used for clinical applications, including tissue engineering.

摘要翻译： 使非均质细胞群体（一个具有干细胞和非干细胞两者）对极端应激选择性地消除非干细胞，并导致群体中干细胞的富集。 压力可以有多种形式，包括但不限于细胞毒素，高温，高盐和低氧（缺氧）条件。 应激后残留的干细胞数量增加，传统干细胞标志物表达增加。 显示干细胞能够增殖和分化成多种类型的细胞。 该方法允许大规模从成体异质细胞群体纯化干细胞，而不需要昂贵的设备，并且不需要细胞表面标记物的存在。 通过上述方法制备的干细胞可用于临床应用，包括组织工程。