公开(公告)号：US20060188892A1

公开(公告)日：2006-08-24

申请号：US11076455

申请日：2005-03-09

申请人： Gary Latham ,  Brittan Pasloske ,  Heidi Peltier

发明人： Gary Latham ,  Brittan Pasloske ,  Heidi Peltier

IPC分类号： C12Q1/68 ,  C12N1/08

CPC分类号： C12Q1/6806 ,  C12N15/1003

摘要： The present invention concerns a compositions and method for isolating a nucleic acid from a cell-containing sample. There is disclosed a method comprising obtaining at least one cell-containing sample, which comprises a cell containing nucleic acid, obtaining at least one catabolic enzyme, obtaining at least one nuclease inhibitor, preparing an admixture of the sample, the catabolic enzyme, and the nuclease inhibitor, maintaining the admixture under conditions where the catabolic enzyme is active, and agitating the admixture, where the sample is digested to produce a nucleic acid-containing lysate of the sample.

摘要翻译： 本发明涉及从含细胞的样品中分离核酸的组合物和方法。 公开了一种方法，包括获得至少一种含细胞的样品，其包含含有核酸的细胞，获得至少一种分解代谢酶，获得至少一种核酸酶抑制剂，制备样品，分解代谢酶和 核酸酶抑制剂，在分解代谢酶活性的条件下保持混合物，并搅拌混合物，其中样品被消化以产生样品的含核酸裂解物。

公开(公告)号：US20090286304A1

公开(公告)日：2009-11-19

申请号：US12463780

申请日：2009-05-11

申请人： Gary Latham ,  Brittan L. Pasloske ,  Heidi Peltier

发明人： Gary Latham ,  Brittan L. Pasloske ,  Heidi Peltier

IPC分类号： C12N1/08

CPC分类号： C12Q1/6806 ,  C12N15/1003

摘要： The present invention concerns a compositions and method for isolating a nucleic acid from a cell-containing sample. There is disclosed a method comprising obtaining at least one cell-containing sample, which comprises a cell containing nucleic acid, obtaining at least one catabolic enzyme, obtaining at least one nuclease inhibitor, preparing an admixture of the sample, the catabolic enzyme, and the nuclease inhibitor, maintaining the admixture under conditions where the catabolic enzyme is active, and agitating the admixture, where the sample is digested to produce a nucleic acid-containing lysate of the sample.

摘要翻译： 本发明涉及从含细胞的样品中分离核酸的组合物和方法。 公开了一种方法，包括获得至少一种含细胞的样品，其包含含有核酸的细胞，获得至少一种分解代谢酶，获得至少一种核酸酶抑制剂，制备样品，分解代谢酶和 核酸酶抑制剂，在分解代谢酶活性的条件下保持混合物，并搅拌混合物，其中样品被消化以产生样品的含核酸裂解物。