公开(公告)号：US20160258009A1

公开(公告)日：2016-09-08

申请号：US15162263

申请日：2016-05-23

Applicant: GEN-PROBE INCORPORATED

Inventor： Daniel L. KACIAN ,  Kenneth A. BROWNE

IPC: C12Q1/68

CPC classification number: C12Q1/6851 ,  C12Q1/6813 ,  C12Q1/6865 ,  C12Q2545/101 ,  C12Q2537/143

Abstract: Disclosed are compositions and methods for making differentiable amplicon species at unequal ratios using a single amplification system in a single vessel. The number of differentiable amplicons and their ratios to one another are chosen to span the required linear dynamic range for the amplification reaction and to accommodate limitations of the measuring system used to determine the amount of amplicon generated. Unequal amounts of distinguishable amplicon species are generated by providing unequal amounts of one or more amplification reaction components (e.g., distinguishable amplification oligomers, natural and unnatural NTP in an NTP mix, or the like). The amount of target nucleic acid present in a test sample is determined using the linear detection range generated from detection of one or more amplicon species having an amount within the dynamic range of detection.

公开(公告)号：US20140231710A1

公开(公告)日：2014-08-21

申请号：US14261263

申请日：2014-04-24

Applicant: Gen-Probe Incorporated

Inventor： Norman C. NELSON ,  Kenneth A. BROWNE ,  Lizhong DAI ,  James RUSSELL ,  Mark E. FILIPOWSKY ,  Margarita B. KAMINSKY ,  Daniel L. KACIAN

IPC: C12Q1/68

CPC classification number: C12Q1/68 ,  A61L2/186 ,  C11D3/3956 ,  C12Q1/6848

Abstract: The disclosure relates to formulations for use in deactivating nucleic acids and methods of making and using the same.

Abstract translation: 本公开涉及用于减活核酸的制剂及其制备和使用方法。