公开(公告)号：US20030054553A1

公开(公告)日：2003-03-20

申请号：US09910828

申请日：2001-07-23

Applicant: GenVec, Inc.

Inventor： Douglas E. Brough ,  Jason G.D. Gall ,  Imre Kovesdi

IPC: C12N015/861 ,  C12N007/00 ,  C12N005/08

CPC classification number: C12N15/86 ,  C12N7/00 ,  C12N2710/10343 ,  C12N2710/10352

Abstract: The invention provides cells, particularly NCI-H460 cells and Calu-1 cells, for the propagation of replication-deficient adenoviral vectors. The cells are lung carcinomas with either a wild-type p53 gene or a heterozygous K-ras mutation. The cells comprise at least one adenoviral nucleic acid sequence, which upon expression produces a gene product that complements for at least one essential gene function of one or more regions of an adenoviral genome so as to propagate a replication-deficient adenoviral vector comprising an adenoviral genome deficient in the at least one essential gene function of the one or more regions when present in the cell.

Abstract translation: 本发明提供用于增殖复制缺陷型腺病毒载体的细胞，特别是NCI-H460细胞和Calu-1细胞。 细胞是具有野生型p53基因或杂合K-ras突变的肺癌。 细胞包含至少一种腺病毒核酸序列，其在表达时产生补充腺病毒基因组的一个或多个区域的至少一个必需基因功能的基因产物，以便繁殖包含腺病毒基因组的复制缺陷型腺病毒载体 当存在于细胞中时，一个或多个区域的至少一个必需基因功能缺陷。