公开(公告)号：US20240183860A1

公开(公告)日：2024-06-06

申请号：US18493957

申请日：2023-10-25

Applicant: Genentech, Inc.

Inventor： Jian Mehr-Dean Payandeh ,  Patrick C. Reid ,  Christian Nathaniel Cunningham ,  Evan Green ,  Christopher Koth ,  Vishal Verma

IPC: G01N33/68 ,  A61K38/00 ,  C07K7/56

CPC classification number: G01N33/6845 ,  C07K7/56 ,  G01N33/6872 ,  A61K38/00

Abstract: The present disclosure relates to chimeric ABC transporter proteins and methods of screening for molecules that bind to the periplasmic, extracellular, and/or luminal face of an ABC transporter protein using the chimeric ABC transporters. For example, in some embodiments, screening methods involve providing a chimeric ABC transporter in which one or more regions of the periplasmic, extracellular, and/or luminal face of the ABC transporter are substituted with one or more equivalent regions of the periplasmic, extracellular, and/or luminal face of a different ABC transporter and selecting for molecules that bind to the ABC transporter but do not bind to the chimeric ABC transporter. The disclosure also relates to molecules that bind to the periplasmic, extracellular, and/or luminal face of an ABC transporter protein, for example, identified in such screens.

公开(公告)号：US20230368863A1

公开(公告)日：2023-11-16

申请号：US18338772

申请日：2023-06-21

Applicant: Genentech, Inc.

Inventor： Man-Ling Lee ,  Alberto Emilio Gobbi ,  Christian Nathaniel Cunningham

IPC: G16B15/30 ,  G16B15/20 ,  G16B40/00

CPC classification number: G16B15/30 ,  G16B15/20 ,  G16B40/00

Abstract: Methods, systems, and computer program products are provided for clustering of similar peptides to detect candidates for target binding. In some embodiments, a method provided herein includes receiving sequencing information and quantification information of a plurality of peptides after target-binding selection in a library. The sequencing information includes amino acid sequences of the plurality of peptides, and the quantification information includes a count of copies of each amino acid sequence in the plurality of peptides. The method further includes computing similarity scores for pairs of the plurality of peptides using the sequencing information. The method further includes grouping the plurality of peptides into clusters based on the similarity scores. The method further includes screening the clusters based on quantification information of peptides in each cluster to obtain candidates for target binding over a pre-set threshold.