公开(公告)号：US20240183860A1

公开(公告)日：2024-06-06

申请号：US18493957

申请日：2023-10-25

Applicant: Genentech, Inc.

Inventor： Jian Mehr-Dean Payandeh ,  Patrick C. Reid ,  Christian Nathaniel Cunningham ,  Evan Green ,  Christopher Koth ,  Vishal Verma

IPC: G01N33/68 ,  A61K38/00 ,  C07K7/56

CPC classification number: G01N33/6845 ,  C07K7/56 ,  G01N33/6872 ,  A61K38/00

Abstract: The present disclosure relates to chimeric ABC transporter proteins and methods of screening for molecules that bind to the periplasmic, extracellular, and/or luminal face of an ABC transporter protein using the chimeric ABC transporters. For example, in some embodiments, screening methods involve providing a chimeric ABC transporter in which one or more regions of the periplasmic, extracellular, and/or luminal face of the ABC transporter are substituted with one or more equivalent regions of the periplasmic, extracellular, and/or luminal face of a different ABC transporter and selecting for molecules that bind to the ABC transporter but do not bind to the chimeric ABC transporter. The disclosure also relates to molecules that bind to the periplasmic, extracellular, and/or luminal face of an ABC transporter protein, for example, identified in such screens.