公开(公告)号：US20180002655A1

公开(公告)日：2018-01-04

申请号：US15545395

申请日：2016-01-29

Applicant: GENERAL ELECTRIC COMPANY

Inventor： Haresh Digambar Patil ,  Manoj Ramakrishna ,  Anoop Bhargav ,  Praveen Paul ,  Sebastian John ,  Swapnil Puranik

IPC: C12M1/36 ,  C12M3/06 ,  C12M1/12 ,  C12M1/00 ,  C12N5/071

CPC classification number: C12M41/48 ,  B01F11/0017 ,  B01F15/0085 ,  C12M23/04 ,  C12M23/14 ,  C12M23/38 ,  C12M23/52 ,  C12M27/16 ,  C12M29/10 ,  C12M41/12 ,  C12M41/14 ,  C12N5/0602 ,  C12N2523/00 ,  C12N2527/00

Abstract: The present invention relates to a novel bioreactor system for cell cultivation. More specifically, the invention relates to a compact bioreactor system which has several integrated functions and enables small scale static culture as well as scale-up rocking culture in the same bioreactor. The bioreactor system comprises tray for positioning of a cell culture bag having adjustable volume, a lid covering the cell culture bag and provided with heating function, an integrated perfusion unit, an integrated cell loading unit, and an integrated unit for automatic cell culture sampling, wherein the bioreactor system is controlled by a single control unit. The invention also relates to a method of cell culture using the bioreactor system for culture of therapeutic cells.

公开(公告)号：US20200181560A1

公开(公告)日：2020-06-11

申请号：US16316297

申请日：2017-06-22

Applicant: General Electric Company

Inventor： Prashanth Hosabettu Mohan ,  Sandip Maity ,  Nagapriya Kavoori Sethumadhavan ,  Swapnil Puranik ,  Li Ou

IPC: C12M1/34 ,  C12M3/06 ,  C12M1/36

Abstract: A microfluidic device (100) is disclosed for in-process monitoring of cell culture conditions including for example one or more of: cell density; cell viability; secreted proteins; protein analysis; epitope markers; concentrations of metabolites or nutrients and antigenic determinations; the device comprising: a cell inlet path (120); plural fluid reservoirs (130) in fluid communication with the cell input path, a cell analysis area (160) in fluid communication with the path and reservoirs, and a waste storage volume (166) also in fluid communication with the cell analysis area, the device being operable to cause a primary fluid flow along the inlet path to the analysis area, and to selectively cause secondary fluid flow(s) into the path from none, one or more of the selected reservoirs to combine, if one or more of the reservoirs are selected, with the primary fluid flow from the cell inlet path, in each case for analysis at the cell analysis area, the device being further operable to cause a fluid flow of the primary and any combined secondary flows from the cell analysis area into the waste storage volume.