公开(公告)号：US10472620B2

公开(公告)日：2019-11-12

申请号：US14321160

申请日：2014-07-01

申请人： General Electric Company

发明人： John Richard Nelson ,  David Roger Moore ,  Robert Scott Duthie ,  Matthew Jeremiah Misner ,  Gregory Andrew Grossmann ,  Elizabeth Marie Dees ,  Patrick McCoy Spooner ,  Erik Leeming Kvam ,  Andrew Arthur Paul Burns ,  Vicki Herzl Watkins

IPC分类号： C12P19/34 ,  C12N15/10

摘要： A method is provided herein, the method includes: applying a sample comprising target nucleic acids to a sample application zone of a substrate; and flowing a nucleic acid amplification reaction mixture across a length of the substrate through the sample application zone to amplify the target nucleic acid forming a nucleic acid amplification product; wherein the target nucleic acid having a first molecular weight is substantially immobilized at the sample application zone and wherein the amplification product having a second molecular weight migrates away from the sample application zone. An associated device is also provided.

公开(公告)号：US20160002621A1

公开(公告)日：2016-01-07

申请号：US14321160

申请日：2014-07-01

申请人： General Electric Company

发明人： John Richard Nelson ,  David Roger Moore ,  Robert Scott Duthie ,  Matthew Jeremiah Misner ,  Gregory Andrew Grossmann ,  Elizabeth Marie Dees ,  Patrick McCoy Spooner ,  Erik Leeming Kvam ,  Andrew Arthur Paul Burns ,  Vicki Herzl Watkins

IPC分类号： C12N15/10

CPC分类号： C12N15/1006 ,  C12Q1/6834 ,  C12Q2565/625

摘要： A method is provided herein, the method includes: applying a sample comprising target nucleic acids to a sample application zone of a substrate; and flowing a nucleic acid amplification reaction mixture across a length of the substrate through the sample application zone to amplify the target nucleic acid forming a nucleic acid amplification product; wherein the target nucleic acid having a first molecular weight is substantially immobilized at the sample application zone and wherein the amplification product having a second molecular weight migrates away from the sample application zone. An associated device is also provided.

摘要翻译： 本文提供了一种方法，所述方法包括：将包含靶核酸的样品施加到底物的样品施用区; 并使核酸扩增反应混合物通过样品施用区跨越一定长度的底物扩增形成核酸扩增产物的靶核酸; 其中具有第一分子量的靶核酸基本上固定在样品施用区，并且其中具有第二分子量的扩增产物从样品施用区迁移。 还提供了相关联的设备。