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1.发明申请公开(公告)号：US20180305718A1
公开(公告)日：2018-10-25
申请号：US15491125
申请日：2017-04-19
发明人： John Richard Nelson , Robert Scott Duthie , Patrick McCoy Spooner , John Anthony Schiel , Lisa Anne Lowery , Anja Josifa Smith
CPC分类号： C12N15/907 , C12N15/102 , C12N15/11 , C12N2310/20
摘要： A method of site-specific modification of an endogenous target DNA of a eukaryotic cell is provided. The method includes contacting the endogenous target DNA having an intended modification site with (i) a gene editing system configured to introduce a double strand break in the endogenous target DNA at or near the intended modification site, and (ii) a donor DNA repair template comprising a plurality of tandem repeat sequences. In the method, each of the plurality of tandem repeat sequences comprises an exogenous donor DNA sequence flanked by a donor 5′ flanking sequence and a donor 3′ flanking sequence. The donor 5′ flanking sequence and the donor 3′ flanking sequence are homologous to a continuous DNA sequence on either side of the intended modification site in the endogenous target DNA.
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公开(公告)号：US20160289735A1
公开(公告)日：2016-10-06
申请号：US14672358
申请日：2015-03-30
CPC分类号： C12Q1/6804 , C12N15/1003 , C12Q2522/101 , G01N33/689
摘要： A method is provided herein, wherein the method of capturing a sperm deoxyribo nucleic acid (DNA) in a sample, comprises contacting a lysis solution to the sample comprising at least a sperm cell or a sperm cell lysate to lyse the sperm cell. The sperm cell or sperm cell lysate comprises a protamine-DNA complex. The method further comprises applying at least a protamine-specific antibody to the lysed sperm cell, wherein the protamine-specific antibody binds to the protamine-DNA complex of the lysed sperm cell to form an antibody-protamine-DNA complex. The antibody binding is followed by capturing the antibody-protamine-DNA complex; and isolating and detecting the sperm DNA from the captured antibody-protamine-DNA complex.
摘要翻译： 本文提供了一种方法，其中在样品中捕获精子脱氧核糖核酸（DNA）的方法包括将裂解液与包含至少精子细胞或精细胞裂解物的样品接触以裂解精子细胞。 精子细胞或精子细胞裂解物包含鱼精蛋白-DNA复合物。 该方法还包括向裂解的精子细胞施加至少一种鱼精蛋白特异性抗体，其中鱼精蛋白特异性抗体结合裂解的精细胞的鱼精蛋白-DNA复合物以形成抗体 - 鱼精蛋白-DNA复合物。 抗体结合后，捕获抗体 - 鱼精蛋白-DNA复合物; 并从捕获的抗体 - 鱼精蛋白-DNA复合物中分离和检测精子DNA。
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公开(公告)号：US09063041B2
公开(公告)日：2015-06-23
申请号：US13690801
申请日：2012-11-30
CPC分类号： G01N1/30 , A01N1/0242 , A01N1/0284 , B01L3/5023 , B01L3/5088 , B01L7/00 , B01L2200/0678 , B01L2300/0825 , B01L2300/126 , B01L2300/1877 , G01N2001/4027 , Y10T436/2525
摘要： A method of drying a biological sample disposed on a substrate is provided. The method comprises providing the substrate comprising a sample loading area and a heat source; activating the heat source for generating heat; heating the substrate at least above 65° C.; and drying the biological sample. A device for storing sample is also provided, wherein the device comprises a substrate for biological sample-storage; and a heating component that generates heat to maintain a temperature of at least above 65° C. The heating component may contain one or more reagents, wherein the reagents generate heat to maintain a temperature of at least above 65° C.
摘要翻译： 提供了一种干燥设置在基板上的生物样品的方法。 该方法包括提供包括样品加载区域和热源的基底; 激活热源产生热量; 将衬底加热至少高于65℃; 并干燥生物样品。 还提供了用于存储样品的装置，其中所述装置包括用于生物样品储存的基底; 以及产生热量以保持至少高于65℃的温度的加热组分。加热组分可以含有一种或多种试剂，其中试剂产生热量以保持至少高于65℃的温度。
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公开(公告)号：US10472620B2
公开(公告)日：2019-11-12
申请号：US14321160
申请日：2014-07-01
发明人： John Richard Nelson , David Roger Moore , Robert Scott Duthie , Matthew Jeremiah Misner , Gregory Andrew Grossmann , Elizabeth Marie Dees , Patrick McCoy Spooner , Erik Leeming Kvam , Andrew Arthur Paul Burns , Vicki Herzl Watkins
摘要： A method is provided herein, the method includes: applying a sample comprising target nucleic acids to a sample application zone of a substrate; and flowing a nucleic acid amplification reaction mixture across a length of the substrate through the sample application zone to amplify the target nucleic acid forming a nucleic acid amplification product; wherein the target nucleic acid having a first molecular weight is substantially immobilized at the sample application zone and wherein the amplification product having a second molecular weight migrates away from the sample application zone. An associated device is also provided.
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公开(公告)号：US20190003940A1
公开(公告)日：2019-01-03
申请号：US15639511
申请日：2017-06-30
发明人： Arunkumar Natarajan , John Richard Nelson , Patrick McCoy Spooner , Ralf Lenigk , Wei Gao , Kwok Pong Chan , Lakshmi Sireesha Kaanumalle , Abraham Joy , Nicholas Nun
摘要： A method includes providing a biological sample, providing a sample collection device, wherein the sample collection device includes a sample binding surface including a photodegradable polymer configured to bind the biological sample, contacting the biological sample with the sample binding surface of the sample collection device, and irradiating the sample binding surface and the bound biological sample using light emitted from a light source to initiate degradation of the photodegradable polymer of the sample binding surface to cause release of the biological sample.
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公开(公告)号：US20150167065A1
公开(公告)日：2015-06-18
申请号：US14106264
申请日：2013-12-13
发明人： John Richard Nelson , David Roger Moore , Bing Li , Robert Scott Duthie , Patrick McCoy Spooner
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6844 , C12Q1/6853 , C12Q2521/307 , C12Q2531/119
摘要： Provided herein are methods for amplification a target dsDNA that is impregnated within a porous matrix using endonuclease-assisted DNA amplification. The amplicons may be subsequent detected within the porous matrix or may be eluted out of the porous matrix. Methods for extracting a genetic material from a biological sample using endonuclease-assisted DNA amplification within a porous matrix are also provided.
摘要翻译： 本文提供了使用内切核酸酶辅助的DNA扩增来扩增浸渍在多孔基质内的靶dsDNA的方法。 可以在多孔基质内随后检测扩增子，或者可以从多孔基质中洗脱。 还提供了在多孔基质内使用内切核酸酶辅助的DNA扩增从生物样品中提取遗传物质的方法。
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公开(公告)号：US10598577B2
公开(公告)日：2020-03-24
申请号：US15639511
申请日：2017-06-30
发明人： Arunkumar Natarajan , John Richard Nelson , Patrick McCoy Spooner , Ralf Lenigk , Wei Gao , Kwok Pong Chan , Lakshmi Sireesha Kaanumalle , Abraham Joy , Nicholas Nun
摘要： A method includes providing a biological sample, providing a sample collection device, wherein the sample collection device includes a sample binding surface including a photodegradable polymer configured to bind the biological sample, contacting the biological sample with the sample binding surface of the sample collection device, and irradiating the sample binding surface and the bound biological sample using light emitted from a light source to initiate degradation of the photodegradable polymer of the sample binding surface to cause release of the biological sample.
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公开(公告)号：US20180361317A1
公开(公告)日：2018-12-20
申请号：US16012147
申请日：2018-06-19
发明人： Christopher Michael Puleo , John Richard Nelson , Patrick McCoy Spooner , Ralf Lenigk , Nicole Lea Wood , Li Zhu , Craig Patrick Galligan
摘要： A method of eluting biomolecules, such as nucleic acids from a biological sample by electroelution is provided. An example of a method includes various steps, such as loading the biological sample to a device comprising a housing, at least two conductive redox polymer electrodes operationally coupled to the housing and a biomolecule impermeable layer disposed on at least one of the electrodes. The loading of sample is followed by initiating an electrical connection to generate an electric field strength sufficient to elute biomolecules from the biological sample; and eluting the biomolecules from the biological sample.
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公开(公告)号：US20160002715A1
公开(公告)日：2016-01-07
申请号：US14321235
申请日：2014-07-01
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6848 , C12Q2531/119 , C12Q2531/125 , C12Q2565/518
摘要： A method is provided herein, the method includes: applying a sample comprising target nucleic acids to a sample application zone of a substrate; applying an aqueous buffer to the sample application zone of the substrate to washes away one or more inhibitors present on the sample application zone; and applying an isothermal nucleic acid amplification reaction mixture to the sample application zone to amplify the target nucleic acid to form a nucleic acid amplification product. The target nucleic acid having a first molecular weight is substantially immobilized at the sample application zone and wherein the amplification product having a second molecular weight.
摘要翻译： 本文提供了一种方法，所述方法包括：将包含靶核酸的样品施加到底物的样品施用区; 将水性缓冲液施加到基材的样品施加区域以洗涤存在于样品施加区上的一种或多种抑制剂; 以及将等温核酸扩增反应混合物应用于样品施用区域以扩增靶核酸以形成核酸扩增产物。 具有第一分子量的靶核酸基本上固定在样品施用区，并且其中扩增产物具有第二分子量。
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公开(公告)号：US20140154736A1
公开(公告)日：2014-06-05
申请号：US13690801
申请日：2012-11-30
CPC分类号： G01N1/30 , A01N1/0242 , A01N1/0284 , B01L3/5023 , B01L3/5088 , B01L7/00 , B01L2200/0678 , B01L2300/0825 , B01L2300/126 , B01L2300/1877 , G01N2001/4027 , Y10T436/2525
摘要： A method of drying a biological sample disposed on a substrate is provided. The method comprises providing the substrate comprising a sample loading area and a heat source; activating the heat source for generating heat; heating the substrate at least above 65° C.; and drying the biological sample. A device for storing sample is also provided, wherein the device comprises a substrate for biological sample-storage; and a heating component that generates heat to maintain a temperature of at least above 65° C. The heating component may contain one or more reagents, wherein the reagents generate heat to maintain a temperature of at least above 65° C.
摘要翻译： 提供了一种干燥设置在基板上的生物样品的方法。 该方法包括提供包括样品加载区域和热源的基底; 激活热源产生热量; 将衬底加热至少高于65℃; 并干燥生物样品。 还提供了用于存储样品的装置，其中所述装置包括用于生物样品储存的基底; 以及产生热量以保持至少高于65℃的温度的加热组分。加热组分可以含有一种或多种试剂，其中试剂产生热量以保持至少高于65℃的温度。
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