公开(公告)号：US20170159117A1

公开(公告)日：2017-06-08

申请号：US15435206

申请日：2017-02-16

Applicant: Genia Technologies, Inc.

Inventor： Randall W Davis ,  Roger J.A. Chen

IPC: C12Q1/68 ,  G01N33/487 ,  G01N27/447 ,  B82Y15/00

CPC classification number: C12Q1/6869 ,  B82Y10/00 ,  B82Y15/00 ,  C12Q1/6816 ,  C12Q1/6825 ,  G01N27/447 ,  G01N33/48721 ,  Y10S977/924 ,  C12Q2525/119 ,  C12Q2525/121 ,  C12Q2525/204 ,  C12Q2527/101 ,  C12Q2563/149 ,  C12Q2565/631

Abstract: The present invention relates to a method of using nanopores to obtain sequence information of sample DNAs in ss test DNAs. The method comprises using speed bumps to stall the ss test DNAs in the nanopores at random positions of the ss test DNAs to obtain sequence information of each and every nucleotides of the sample DNAs, and to construct the whole sequences of the sample DNAs. The present invention also relates to identification and/or isolation of test DNAs having desired sequence(s) using nanopore detectors facilitated by speed bump.

公开(公告)号：US10400278B2

公开(公告)日：2019-09-03

申请号：US15435206

申请日：2017-02-16

Applicant: Genia Technologies, Inc.

Inventor： Randall W Davis ,  Roger J. A. Chen

IPC: C12Q1/6869 ,  C12Q1/6816 ,  G01N33/487 ,  B82Y15/00 ,  G01N27/447 ,  C12Q1/6825 ,  B82Y10/00

Abstract: The present invention relates to a method of using nanopores to obtain sequence information of sample DNAs in ss test DNAs. The method comprises using speed bumps to stall the ss test DNAs in the nanopores at random positions of the ss test DNAs to obtain sequence information of each and every nucleotides of the sample DNAs, and to construct the whole sequences of the sample DNAs. The present invention also relates to identification and/or isolation of test DNAs having desired sequence(s) using nanopore detectors facilitated by speed bump.