公开(公告)号：US11814664B2

公开(公告)日：2023-11-14

申请号：US14893510

申请日：2014-05-23

Applicant: Genomatica, Inc.

Inventor： Benjamin Adelstein ,  Robin E. Osterhout ,  Mark J. Burk

IPC: C12P7/62 ,  C12N9/18 ,  A61K31/22 ,  A61K35/66 ,  A61K36/064 ,  A23L33/135 ,  C12N1/00 ,  C12N1/14 ,  C12N1/20 ,  C07C69/675 ,  C12N15/52 ,  C12R1/00 ,  C12R1/01 ,  C12R1/645

CPC classification number: C12P7/62 ,  A23L33/135 ,  A61K31/22 ,  A61K35/66 ,  A61K36/064 ,  C07C69/675 ,  C12N1/00 ,  C12N1/145 ,  C12N1/205 ,  C12N9/18 ,  C12N15/52 ,  C12Y101/011 ,  C12Y101/01035 ,  C12Y301/01067 ,  C12Y301/08001 ,  A23V2002/00 ,  C07B2200/05 ,  C12R2001/00 ,  C12R2001/01 ,  C12R2001/645 ,  Y02P20/10

Abstract: Provided herein are non-naturally occurring microbial organisms having a pathway for production of (3R)-hydroxybutyl (3R)-hydroxybutyrate, wherein the organism can further include a (R)-1,3-butanediol pathway, a (3R)-hydroxybutyrate pathway, a (3R)-hydroxybutyryl-CoA pathway, an acetoacetate pathway, an acetoacetyl-CoA pathway, a (3R)-hydroxybutyl-ACP pathway, or an acetoacetyl-ACP pathway. Additionally provided are methods and processes for producing and isolating (3R)-hydroxybutyl (3R)-hydroxybutyrate using the microbial organisms, and various compositions having the (3R)-hydroxybutyl (3R)-hydroxybutyrate. Still further provided are methods of treating or preventing a disease, disorder or condition using the (3R)-hydroxybutyl (3R)-hydroxybutyrate produced by the microbial organisms of the invention.