公开(公告)号：US11898172B2

公开(公告)日：2024-02-13

申请号：US16498336

申请日：2018-03-29

申请人： Genomatica, Inc.

发明人： Kevin Hoff ,  Cara Ann Tracewell ,  Kui Chan ,  Michael Kuchinskas ,  Harish Nagarajan

IPC分类号： C12N9/04 ,  C12N9/10 ,  C12P7/18 ,  C12P7/24 ,  C12P7/52 ,  C12P19/32

CPC分类号： C12N9/0006 ,  C12N9/1029 ,  C12P7/18 ,  C12P7/24 ,  C12P7/52 ,  C12P19/32 ,  C12Y101/01157 ,  C12Y203/01009

摘要： The present disclosure provides thiolases and polypeptide variants of 3-hydroxybutyryl-CoA dehydrogenase, nucleic acids encoding the same, vectors comprising the nucleic acids, and cells comprising the polypeptide variants and/or thiolase, the nucleic acids, and/or the vectors. The present disclosure also provides methods of making and using the same, including methods for culturing cells, and for the production of various products, including 3-hydroxybutyryl-CoA (3-HB-CoA), 3-hydroxybutyraldehyde (3-HBal), 3-hydroxybutyrate (3-HB), 1,3-butanediol (1,3-BDO), and esters and amides thereof, and products made from any of these.

公开(公告)号：US20220325254A1

公开(公告)日：2022-10-13

申请号：US17697504

申请日：2022-03-17

申请人： Genomatica, Inc.

发明人： Kevin Hoff ,  Cara Ann Tracewell ,  Kui Chan ,  Amit Shah ,  Joseph Warner ,  Michael Noble

IPC分类号： C12N9/02

摘要： The invention provides polypeptides and encoding nucleic acids of aldehyde dehydrogenase variants. The invention also provides cells expressing aldehyde dehydrogenase variants. The invention further provides methods for producing 3-hydroxybutyraldehyde (3-HBal) and/or 1,3-butanediol (1,3-BDO), or an ester or amide thereof, comprising culturing cells expressing an aldehyde dehydrogenase variant or using lysates of such cells. The invention additional provides methods for producing 4-hydroxybutyraldehyde (4-HBal) and/or 1,4-butanediol (1,4-BDO), or an ester or amide thereof, comprising culturing cells expressing an aldehyde dehydrogenase variant or using lysates of such cells.

公开(公告)号：US20200087634A1

公开(公告)日：2020-03-19

申请号：US16498336

申请日：2018-03-29

申请人： Genomatica, Inc.

发明人： Kevin Hoff ,  Cara Ann Tracewell ,  Kui Chan ,  Michael Kuchinkas ,  Harish Nagarajan

IPC分类号： C12N9/04 ,  C12N9/10 ,  C12P19/32 ,  C12P7/52 ,  C12P7/24 ,  C12P7/18

摘要： The present disclosure provides thiolases and polypeptide variants of 3-hydroxybutyryl-CoA dehydrogenase, nucleic acids encoding the same, vectors comprising the nucleic acids, and cells comprising the polypeptide variants and/or thiolase, the nucleic acids, and/or the vectors. The present disclosure also provides methods of making and using the same, including methods for culturing cells, and for the production of various products, including 3-hydroxybutyryl-CoA (3-HB-CoA), 3-hydroxybutyraldehyde (3-HBal), 3-hydroxybutyrate (3-HB), 1,3-butanediol (1,3-BDO), and esters and amides thereof, and products made from any of these.

公开(公告)号：US20230139515A1

公开(公告)日：2023-05-04

申请号：US17812636

申请日：2022-07-14

申请人： Genomatica, Inc.

发明人： Kevin Hoff ,  Cara Ann Tracewell ,  Kui Chan ,  Michael Kuchinskas ,  Harish Nagarajan

IPC分类号： C12N9/04 ,  C12N9/10 ,  C12P7/18 ,  C12P7/24 ,  C12P7/52 ,  C12P19/32

摘要： The present disclosure provides thiolases and polypeptide variants of 3-hydroxybutyryl-CoA dehydrogenase, nucleic acids encoding the same, vectors comprising the nucleic acids, and cells comprising the polypeptide variants and/or thiolase, the nucleic acids, and/or the vectors. The present disclosure also provides methods of making and using the same, including methods for culturing cells, and for the production of various products, including 3-hydroxybutyryl-CoA (3-HB-CoA), 3-hydroxybutyraldehyde (3-HBal), 3-hydroxybutyrate (3-HB), 1,3-butanediol (1,3-BDO), and esters and amides thereof, and products made from any of these.

公开(公告)号：US11299716B2

公开(公告)日：2022-04-12

申请号：US16499531

申请日：2018-03-29

申请人： Genomatica, Inc.

发明人： Kevin Hoff ,  Cara Ann Tracewell ,  Kui Chan ,  Amit Shah ,  Joseph Warner ,  Michael Noble

IPC分类号： C12N9/02

摘要： The invention provides polypeptides and encoding nucleic acids of aldehyde dehydrogenase variants. The invention also provides cells expressing aldehyde dehydrogenase variants. The invention further provides methods for producing 3-hydroxybutyraldehyde (3-HBal) and/or 1,3-butanediol (1,3-BDO), or an ester or amide thereof, comprising culturing cells expressing an aldehyde dehydrogenase variant or using lysates of such cells. The invention additional provides methods for producing 4-hydroxybutyraldehyde (4-HBal) and/or 1,4-butanediol (1,4-BDO), or an ester or amide thereof, comprising culturing cells expressing an aldehyde dehydrogenase variant or using lysates of such cells.

公开(公告)号：US20200040312A1

公开(公告)日：2020-02-06

申请号：US16499531

申请日：2018-03-29

申请人： Genomatica, Inc.

发明人： Kevin Hoff ,  Cara Ann Tracewell ,  Kui Chan ,  Amit Shah ,  Joseph Warner ,  Michael Noble

IPC分类号： C12N9/02

摘要： The invention provides polypeptides and encoding nucleic acids of aldehyde dehydrogenase variants. The invention also provides cells expressing aldehyde dehydrogenase variants. The invention further provides methods for producing 3-hydroxybutyraldehyde (3-HBal) and/or 1,3-butanediol (1,3-BDO), or an ester or amide thereof, comprising culturing cells expressing an aldehyde dehydrogenase variant or using lysates of such cells. The invention additional provides methods for producing 4-hydroxybutyraldehyde (4-HBal) and/or 1,4-butanediol (1,4-BDO), or an ester or amide thereof, comprising culturing cells expressing an aldehyde dehydrogenase variant or using lysates of such cells.